Objective:To introduce the use of fluoxetine in neurologic disease,especially in epilepsy.Method:We used fluoxetine as a supplement antiepileptic drug in 25 patients who couldnt be controlled yet by routine antiepileptics. A long term follow-up with these cases was carried out.Results:Most patients got better outcome.Conclusion:Fluoxetine would be an important supplement antiepileptic drug and have value for further clinical and experimental research.

Objective To establish HPLC method for fast, effective, simultaneous determination of Vancomycin and Norvancomycin in serum. Methods The separation was achieved on the Hypersil ODS2(250 mm ×4.6 mm,5μm)with methanol-acetonitrile-a mixture of potassium phosphate (9.5:2.5:88) as the mobile phase.The flow rate was 1.0 mL/min.The UV detecting wavelength was 280 nm.The temperature of the column was 35 ℃. Vancomycin and Norvancomycin were used as the internal standard for each other .Results Vancomycin and Norvancomycin blood concentration were in well linearity in the range of 1.25-100μg/mL,R2 =0.9996.The average method recovery rate of three concentrations were 96.88%,99.50%,105.01%respectively, the average method recovery rate of three concentrations were 94.40%,103.90,103.81%respectively, the intra-day and inter-day RSD were less than 5%.Conclusion The HPLC method is proved to be simple,sensitive, accurate and precise in which little blood sample is taken, thus it is suitable for the therapeutic drug monitoring(TDM) and pharmacokinetic study of Vancomycin and Norvancomycin.

There is a lack of knowledge concerning the frequency and significance in patients treated in the NICU because of the confused and difficult techniques in viral testing. Some researches revealed that the incidence of viral infection in the NICU was 5%. We discussed the common viral infections including re-spiratory syncytial virus,enterovirus,rotavirus,cytomegalovirus,herpes simplex virus,rubella virus,parvovir-us and human immunodeficiency virus. Based on the database of the outbreak of viral infections in NICU,we need for more effective outbreak prevention strategies.

With performance evaluation management as the stepping stone,the hospital joined the DRGs with the attending in-charge method by means of enforcing the attending in charge practice,DRGs knowledge training,and identifying problems with DRGs grouped data of the attending physician group, in an effort to explore new methods of medical quality control.Two years of practice provide tools of quality control to strengthen the hospital’s fine management.At the same time,it should also be noticed that DRGs merely diversify management means,as the content and form of which still need to constantly be improved in the course.

To observe the therapeutic effect of acupuncture plus TDP irradiation on proliferative arthritis, 304 patients with proliferative arthritis were randomly divided into treatment group and control group, and treated by acupuncture plus TDP irradiation and routine Tuina respectively. The clinical cure rate and total effective rate in the treatment group were both superior to those in the control group. This therapy has good therapeutic effect and short course of treatment in treating proliferative arthritis, and is one of better therapeutic means in clinic presently.

There was subdiscipline tendency in clinical treatment of diabetes during the Tang and Song Dynasty,and the prescription had begun to take shape,and the syndrome differentiation and treatment of the chronic diseases such as diabetes kept increasing accuracy day by day.The way of statistical analysis,which probed the regular of compatibility of prescription and drugs of diabetes in and before the Tang and Song Dynasty,could provide very valuable referenced prescription and drugs in coming Chinese clinic and scientific research.

Objective: To investigate effects of glucose and free fatty acid at different concentrations on phosphorylation of adenosine-5′-monophosphate activated protein kinase(AMPK) and acetyl CoA carboxylase(ACC) in INS-1 cells,and effects of globular adiponectin on phosphorylation of AMPK and ACC.Methods: INS-1 cells were cultured and treated with 5 mmol/L glucose or 0.25 mmol/L free fatty acids,and time courses and dose responses of different dosages of glucose and fatty acid on phosphorylation of AMPK and ACC were measured.We measured the effects of the pharmacological AMPK activator AICAR(5-aminoimidazole-4-carboxamide-riboside) and globular adiponectin on phosphorylation of AMPK and ACC.Results: Glucose and fatty acid at different concentrations inhibited the phosphorylation of AMPK and ACC at the end of 60 min,but AICAR increased the phosphorylation of AMPK and ACC significantly,while 2.5 mg/L globular adiponectin increased the phosphorylation of AMPK and ACC by 23%(P

Objective To establish a HPLC method of determining chrysin and baicalein in Senmen Oroxyli. Method The contents of chrysin and baicalein were determined by HPLC. The chromatographic conditions were: Kromasi1-C_(18) column (5 ?m, 250 mm?4.6 mm), with methanol-acetonitrile-water-glacial acetic acid (25 : 25 : 50 : 0.2) as the mobile phase, at the flow rate of 1 mL/min, the detecting wavelength at 275 nm and at the room temperature. Results The contents of baicalein and chrysin could be detected by this HPLC method, and got good separation and good linear relationship. RSD were 1.53% and 1.35%, indicating good repeatability, recovery RSD were 1.85% and 1.59%, indicating a good recovery. Conclusion The method is simple, feasible and repeatable, and can be provide reference for quality control of Senmen Oroxyli.

Objective:To explore the diagnostic value of ABI detection in lower limb vasculopathy of diabetic patients.Methods:ABI detection was performed in 44 cases suspected of diabetic lower limb vasculopathy,and the results were compared with that of DSA. Results:There was no statistically significant difference between ABI detection and DSA in the diagnosis of diabetic lower limb vasculopathy.Compared with DSA,the accordance rate,sensitivity and specificity of ABI were 87.31%,86.46%and 82.00%respectively. Conclusion:By ABI detection,lower limb vasculopathy of diabetic patients can be detected.

Objective: To explore the value of combined treatment of advanced hepatic carcinoma. Methods:117 cases with advanced hepatic carcinoma underwent a combined treatment by radio frequency ablation(RF),percutaneous ethanol injecton(PEI),transcatheter hepatic arterial chemoembolization(TACE)and transultrasonic portal vein chemoembolization (SPVE).The comparison was made between this treatment and that of TACE alone in terms of size of tumor ,changes of blood supply and clinical effects before and after each of these two treatments. Results: After three months,Ultrasonographic examination showed that the tumor size decreased by 25.0% was 87.2%.The disappearing rate and decreasing rate of blood supply of tumor was 88.0%; AFP turning-negative rate was 78.3%.The follow-up survey showed that one-year and two-year survival rates for combined therapy were 73.5%and 62.4% respectively ,significantly higher than that for the control TACE group 59.4%and 32.6%. Conclusion: The combined treatments are effective ways for carcinoma treatment. Color Doppler imaging is important to guide and assess the treatment.