At present,there are some problems about teaching acupuncture and massage elective course in colleges of western medicine,which are caused by student's specialty,thinking mode and the teaching material of elective course.To improve the quality of the teaching in acupuncture and massage,it is necessary to reorientate the teaching intention,adjust the course plan.

Apoptosis ; drug effects ; Benzoquinones ; pharmacology ; Carcinoma, Non-Small-Cell Lung ; drug therapy ; metabolism ; pathology ; Drug Resistance, Neoplasm ; HSP90 Heat-Shock Proteins ; antagonists & inhibitors ; Humans ; Lactams, Macrocyclic ; pharmacology ; Lung Neoplasms ; drug therapy ; metabolism ; pathology ; Mutation ; Oncogene Proteins, Fusion ; antagonists & inhibitors ; genetics ; metabolism ; Protein Kinase Inhibitors ; therapeutic use ; Pyrazoles ; therapeutic use ; Pyridines ; therapeutic use ; Triazoles ; pharmacology

Animals ; Carps ; physiology ; Galvanic Skin Response ; drug effects ; physiology ; Skin ; cytology ; Strychnine ; pharmacology ; Synaptic Transmission ; drug effects ; physiology

ObjectiveTo investigate suicidal ideation and its risk factors in patients with borderline personality disorder (BPD),and raise awareness of suicidal risk in BPD.MethodsPatients with BPD were selected from in-patient and out-patient departments as case group(n=84),while 84 subjects were selected as control group,1∶1 matched by gender and age.Barratt impulsiveness scale,Buss-Perry aggression questionnaire,childhood trauma questionnaire,Beck depression inventory,Beck hopelessness scale and Beck scale for suicide ideation were used to measure the two groups.Results(1)In case group,the number of incidence of suicidal occurred was 84.The scores of suicidal ideation in the recent week(4.94±4.57 vs 0.50±0.51),suicidal ideation in the worst situation(20.43±6.58 vs 1.26±2.60) and the total score(25.37±9.78 vs 1.76±3.65) were statistically higher than those in control group (t=8.499,24.972,20.847,all P<0.01).(2) Multiple regression analysis showed that motor impulsivity,physical neglect in childhood,impulsivity,co-morbidity of axis I disorders,physical abuse in childhood,unemployment and unmarried status were risk factors of suicidal ideation in patients with BPD (β=0.876,0.640,0.428,0.350,0.236,0.165,-0.205,all P<0.05).ConclusionThe suicide ideation of patients with BPD are prevalent and intense.Awareness of risk factors for patients with BPD,thereby reducing the risk of suicide.

0.05).【Conclusion】Acupoint application shows a therapeutic effect for bronchial asthma in non-acute attack stage by reducing the attack frequency,and its mechanism may be related to the regulation of cellular immune function thus inhibit the airway inflammatory response mediated by EOS.

Objective To learn research cooperation and evolution process in the field of health management in China so as to provide evidence for decision-making.Methods A total of 2180 literatures were selected to investigate author affiliation,high output author,research cooperation,high frequency keywords,and research hotspot through information visualization tool CiteSpace Ⅱ.Results Domestic health management research literature had showed an increasing trend since 2007 and leveled off during recent 3 years (average annual output 446.7).A total of 4 216 authors contributed to 2 180 publications,resulting in an average number of authors per paper of 1.93.Researchers from all autonomous regions and municipalities in China had their health management research published (top five:Beijing,Jiangsu,Guangdong,Shanghai and Sichuan),expect for Qinghai,Tibet,Guizhou and Hainan.Forty-six authors had an occurrence frequency of ≥5.Forty-four keywords showed an occurrence frequency of ≥15 and centrality degree＞0,including health check-up,health education,hypertension,diabetes,life behavior intervention and student health management.Conclusions Health management research have made a rapid progress in China during recent years.Increasing number of research institutions and teams have involved in health management research.However,scientific research cooperation in the field of health management is relatively loose,and interdisciplinary,cross-reginal and interagency cooperation is insufficient.

Objective To study the incidence of vitamin K deficiency in low-birth weight premature infants and its relationship with intraventricular hemorrhage.Methods We use emzymoimmunoelectrophoresis to detect prophrombin protein precursors(PIVKA-Ⅱ) in vein blood in premature infants

[Objective] To retrospectively analyze the outcome of unrelated umbilical cord blood transplantation in the treatment of aggressive-phase chronic myeloid leukemia.[Methods] Fourteen consecutive patients with aggressive-phase chronic myeloid leukemia were treated with unrelated umbilical cord blood transplantation,thirteen patients were treated with myeloablative unrelated CBT and one patients were treated with nonmyeloablative unrelated CBT.All patients received standard cyclosporine A (CsA) and mycophenolate mofetil(MMF) as a graft-versus-host disease (GVHD) prophylaxis.[Results] 14 patients were all successfully engrafted.The median times for their neutrophil returning to ≥0.5×109/L and for platelet returning to ≥20×109/L were 22.8 days and 37.8 days,respectively.Acute GVHD occurred in 10 of 13 evaluable patients.The grading of acute GVHD was gradeⅡ-Ⅳin 6 patients(46.2 ％).Chronic GVHD occurred in 7 of 11 evaluable patients(63.6％).Relapse occurred in 2 of 15 patients,lextramedullary relapse was included.9 of 14 patients were alive and event-free after CBT.The probability of OS rate at 5 years was 64.3 ％,the probability of DFS rate at 5 years was 5,7.1％.[Conclusion]Unrelated umbilical cord blood transplantation is effective in the treatment of aggressive-phase chronic myeloid leukemia.

Objective To investigate the prevalence of depression and its risk factors in type 2 diabetes mellitus.Methods Beck Depression Inventory (BDI) was used to evaluate depression in 2 966 type 2 diabetes mellitus patients [ male 1 463,female 1 503,age ( 56.4 ± 11.2 ) years,diabetes duration ( 6.3 ± 5.7 ) years ].Depression criteria:≤4 points,no depression group; 5-13 points,mild depression group; 14-20 points,moderate depression group; 21 points or higher,severe depression group.Meanwhile,the demographic and metabolic data and diabetes-related health behaviors were also investigated.The risk factors associated with depression were screened by logistic regression.Results 51％ patients had depression,including 38％ with mild,8％ with moderate,and 5％ with severe depression.Compared with no depression group,depression was correlated with female sex,low-annual income,diabetes education,diabetes treatment,with insulin and peripheral neuropathy( P＜0.01 ).More smoking and younger age were found in severe depression( P＜0.05 ).Partial correlation analysis showed that depression in type 2 diabetes mellitus was positively correlated with female sex,diabetes education,and peripheral neuropathy ; and negatively correlated with age (P＜0.05).Conditional logistic regression equation showed that gender( OR =1.37 ),age ( 20-40 years,OR =1.52 ),diabetes education ( OR =1.51 ),and peripheral neuropathy ( OR =1.87 ) were risk factors for depression.Conclusion Depression is common in type 2 diabetes mellitus.More attention should be paid to screening depression in clinical practice.

Bispecific antibodies have been exploited as both cancer immunodiagnostics and cancer therapeutics, which have shown promises in clinical trials in cancer imaging and therapy. To improve the anti-tumor effect, an scDb (bispecific single-chain diabody) was constructed from the variable domain genes of two scFvs (single-chain variable fragment antibodies) directed against human EGFR (epidermal growth factor receptor) and VEGFR2 (vascular endothelial growth factor receptor 2) extracellular domains. The anti-EGFR/ anti-KDR scDb was constructed into pHEN2 plasmid and expressed in Escherichia coli HB2151 host. After purification by one-step affinity chromatography of IMAC, scDb protein was characterized by Western blotting. The yield of scDb protein was 570 microg per liter medium. scDb bound to EGFR as efficiently as the parental antibody scFv-E10, while a little bit weaker than the parental antibody scFv-AK404R when bound to KDR. In conclusion, the scDb protein could bind both EGFR and KDR specifically and could be applied for further anti-tumor research.