BACKGROUND:Rabbit VX2 hepatocarcinoma models are characterized by expanded and infiltrative growth, abundant blood supply, similar pathological and imaging features to human hepatocelular carcinoma, so this model is ideal for imaging experiments. OBJECTIVE: To establish rabbit VX2 hepatocarcinoma models and compare two different laparotomy methods in establishing rabbit VX2 hepatocarcinoma models. METHODS:Thirty New Zealand rabbits were randomly divided into two groups: traditional laparotomy (tumor particles were directly embedded into the liver with tweezers) and improved laparotomy (tumor particles were injected into the liver through a 15G syringe needle). The growth features of the tumor established in two different ways were compared by ultrasound. RESULTS AND CONCLUSION:The tumor formation rate was 100% in the two groups; the single rate was respectively 50% and 90%; the ectopic implantation rate was 50% and 10%, there were significant differences between two groups (P < 0.05). The mortality rate within 2 weeks was 33% and 0 in the two groups, with significant differences (P < 0.05). The improved laparotomy method is feasible for modeling rabbit VX2 hepatocarcinoma, due to minimal injury, short operation time, high success rate and stable features.

Adult ; Female ; Gas Poisoning ; complications ; Humans ; Male ; Pulmonary Aspergillosis ; complications

Objective To economically investigate tertiary stroke rehabilitation treatment.Methods 3 tertiary rehabilitation networks were set up in Beijing.82 patients with acute cerebrovascular diseases were enrolled into the study according to standardized criteria in all centers and randomized into rehabilitation group and control group.Rehabilitation training was implemented according to standardized procedures.The patients in rehabilitation group were given rehabilitation training in acute stage for 28 days and then underwent long term rehabilitation'program for 6 months in rehabilitation centers or community rehabilitation settings.The patients in control group were only given rehabilitation training in acute stage for 28 days. Altogether 4 evaluations were conducted in one week after the onset and at the end of 1,3,6 months, respectively.Results Significant differences of motor function,ability of daily activities,quality of life evaluation and cost were found in the 1st,3rd and 6th month after the onset of diseases.The rehabilitation group surpassed the control group significantly.At the end of 6 months,the average of Barthel Index of rehabilitation group was 83.18?18.22 while that of control group was 63.69?12.73.At the end of 6 months,total cost of rehabilitation group was(56 420.0?17 831.8)Yuan and that of control group was (64 864.2?18 267.5)Yuan.The rehabilitation group had better cost/effectiveness and cost/utility.There was a significant difference of cost between two groups.The total rehabilitation cost of rehabilitation group was 4613 Yuan,accounting for 15.3%of all the cost.Conclusion The tertiary rehabilitation treatment for stroke patients has achieved better cost/effectiveness and cost/utility.In China pharmaceutical cost constitutes the major part of the stroke therapy while the rehabilitation cost only a small portion,which is significantly different from the situation in developed countries.

Adult ; Aged ; China ; epidemiology ; Female ; Gold ; Humans ; Male ; Middle Aged ; Mining ; Silicosis ; mortality ; Young Adult

Objective To explore the influence of blocking B7/CD28 co-stimulatory pathway by RNA interference on the rejection response in mice heterogeneous heart transplantation and its mecha- nism.Methods siRNA of which sequence specified to CD80 and CD86mRNA was synthesized in vitro respectively and transfected into donor derived myeloid dendritic cells (DCs).The expression levels of CD80 and CD86 mRNA and surface antigen CD80,CD86 were assayed by semi-quantitative reverse transcription polymerase chain reaction and flow cytometry before and after CD80siRNA and CD86siRNA transfection.Seven days prior to heterogeneous heart transplantation in mice,DCs modi- fied by siRNA were transfused into recipients intraveneously (DC interference group).At the same time,group of allograft transplantation,cyclosporine A (CsA)-treated (CsA injected subcutaneously postoperatively,5 mg.kg~(-1)?d~(-1)) group,group of isograft transplantation,and non-interference DC group (transfusion of non-interfered DCs pre-transplanting) were assigned.The graft survivals were individually recorded and the graft rejection grading was pathologically evaluated.Interleukin 2 (IL- 2),interferon?(IFN-?),and IL-10 mRNA expression levels in grafts tissue were determined.Results After siRNA transfected into DC,the expression levels of CD80 and CD86 mRNA were down- regulated significantly and the the antigen CD80~+ and CD86~+ reduced from 84%,67% to 35% and 30% respectively.As compared with groups of allograft and non-interference DC,survival of the grafts was significantly longer in DC interference group (P＜0.01),pathological grade of rejection significantly lower (P＜0.01),IL-2 and IFN-?mRNA expression levels lower,and IL-10 mRNA ex- pression levels higher in grafts tissue (P＜0.01).Conclusion Knocking down the molecule B7 expres- sion level in donor-derived myeloid DCs through RNAi,which could block B7/CD28 co-stimulatory pathway,could exhibit inhibitive effect on rejection response in mice heart transplantation.The mechanism might be due to induction of T lymphocyte anergy and Th cell differentiation deviating to T_H2.

Objective To investigate the functional states of stroke patients using the International Classification of Functioning, Disability and Health (ICF) core sets for stroke. Methods Fifty in-patients with stroke participated in this study. Twenty-five patients less than one month after a stroke were assigned to the acute group, and another 25 patients with more than one month since their stroke were assigned to the sub-acute and chronic group. All the patients were assessed using the Glasgow coma scale, Rankin grading and the Barthel index. The ICF core set for stroke assessment was also used with both groups. The data was analyzed by using a Chi squared frequency test. Results Rankin grades and Barthel indices were significantly different between the two groups, and the functional states in the acute group were better than those in the sub-acute and chronic group. Some of the items, such as consciousness state, functions of structures adjoining the eyes, hearing, substitutive sound, accessory respiration, temperature regulation and protection function of the skin had no significant difference between the groups. The number of patients suffering from disorders of joint motion, joint stability, muscle endurance and control of voluntary movement was significantly greater in the sub-acute and chronic group than in the acute group. Both groups were influenced to different degrees by most of the environmental factors. Conclusion The ICF core sets correctly indicated joint motion, joint stability, muscle endurance and control of voluntary movement difficulties of stroke patients.

Objective: To evaluate the clinical value of positron emission tomography/ computed tomography(PET/CT) with fluorine-18-labeled fluorodeoxyglucose(18F-FDG) in dectecting and diagnosing the recurrence and metastasis of colorectal cancer.Methods: The results of whole-body PET/CT of 68 patients with colorectal cancer were analyzed retrospectively.The average follow-up time was 15.8 months.The diagnosis of recurrence tumor and/or metastasis was based on pathologic examination,colonoscopy,multi-modality imaging and clinical follow-up.Results: Fifty-five of the 68 patients had recurrence and /or metastasis but no recurrence was found in the other 13 cases.The sensitivity,specificity and accuracy of PET/CT in detecting recurrent and metastatic tumor were 96.4%,76.9% and 92.6% respectively.PET/CT successfully detected one or several malignant insidious lesions in 8 cases with negative findings by CT and /or ultrasonography,and it revaeled more malignant lesions than CT and ultrasonography in 30.9%(17/55)of the patients.The clinical therapeutic strategies were changed in 11 case duo to the results of PET/CT,and the influence rate of PET/CT was 16.2%.The metastasis was mainly confined in the liver,lung,peritoneal cavity and retroperitoneal region.In the examination of the 24 patients with increased serum CEA,the positive rate of PET/CT was 91.7%.Conlusion: PET/CT can detect the recurrence and metastasis of colorectal cancer with high sensitivity and accuracy.

Remarkable achievements have been made for lymphocyte infusion of lymphoma and leukemia, especially of lymphoproliferative disease within twenty-first century. The donor lymphocyte infusion or mobilized donor lymphocyte infusion (DLI/DSI) offer an opportunity of second remission for relapse patients post-transplantation. Cytokine-induced killer/DC cytokine-induced killer play an anti-tumor activity beyond non-MHC restricted. Cytotoxic lymphocyte infusion activated by synthetic tumor antigen produces targeted effects of anti-tumor. Transgenic CTL of anti-tumor TCR has brought the dawn in lymphoma and leukemia patients with defective lymphocytes. Maternal lymphocyte infusion play an anti-tumor/viral activity by avoiding the immune barrier of HLA mismatch.

Objective To investigate the relationship of carotid artery atherosclerosis and the plasma level of RAS in hypertension patients.Methods We retrospectively analyzed clinic data of essential hypertension patients treated in the department of cardiology department between Jan 2015 and Mar 2016.Results Carotid artery plaque was associated with age,blood sugar,total cholesterol,low density lipoprotein-cholesterol,and uric acid.The prevalence of carotid artery atherosclerosis increased with increasing level of plasma erect and decubitus position angiotensin Ⅱ and erect position renin (P ＜ 0.05).Logistic regression analysis indicted that age,decubitus position angiotensin Ⅱ,TC,UA was positively associated with carotid atherosclerosis,HDL-C was negatively correlated with carotid atherosclerosis(P ＜ 0.05).Conclusion Carotid artery atherosclerosis is independently associated with Ang Ⅱ.

Objective: To study the constituents in the roots of Picrorhiza scrophulariiflora. Methods: The constituents of P. scrophulariiflora were separated and purified with chromatographic methods. The structures were elucidated by spectroscopic methods and chemical analyses. Results: Ten compounds were isolated from the roots of P. scrophulariiflora and identified as β-sitosterol (1), palmitic acid (2), octacosyl trans-ferulate (3), 3β-hydroxystigmast-5-en-7-one (4), 6β-hydroxystigmast-4-en-3-one (5), caffeic acid methyl ester (6), protocatechuic acid methyl ester (7), vanillic acid (8), caffeic acid (9), and piceoside (10). Conclusion: Compounds 2-7 and 9 are obtained from the plants of Picrorhiza Royle for the first time.