BACKGROUND:Rabbit VX2 hepatocarcinoma models are characterized by expanded and infiltrative growth, abundant blood supply, similar pathological and imaging features to human hepatocelular carcinoma, so this model is ideal for imaging experiments. OBJECTIVE: To establish rabbit VX2 hepatocarcinoma models and compare two different laparotomy methods in establishing rabbit VX2 hepatocarcinoma models. METHODS:Thirty New Zealand rabbits were randomly divided into two groups: traditional laparotomy (tumor particles were directly embedded into the liver with tweezers) and improved laparotomy (tumor particles were injected into the liver through a 15G syringe needle). The growth features of the tumor established in two different ways were compared by ultrasound. RESULTS AND CONCLUSION:The tumor formation rate was 100% in the two groups; the single rate was respectively 50% and 90%; the ectopic implantation rate was 50% and 10%, there were significant differences between two groups (P < 0.05). The mortality rate within 2 weeks was 33% and 0 in the two groups, with significant differences (P < 0.05). The improved laparotomy method is feasible for modeling rabbit VX2 hepatocarcinoma, due to minimal injury, short operation time, high success rate and stable features.

Objective To economically investigate tertiary stroke rehabilitation treatment.Methods 3 tertiary rehabilitation networks were set up in Beijing.82 patients with acute cerebrovascular diseases were enrolled into the study according to standardized criteria in all centers and randomized into rehabilitation group and control group.Rehabilitation training was implemented according to standardized procedures.The patients in rehabilitation group were given rehabilitation training in acute stage for 28 days and then underwent long term rehabilitation'program for 6 months in rehabilitation centers or community rehabilitation settings.The patients in control group were only given rehabilitation training in acute stage for 28 days. Altogether 4 evaluations were conducted in one week after the onset and at the end of 1,3,6 months, respectively.Results Significant differences of motor function,ability of daily activities,quality of life evaluation and cost were found in the 1st,3rd and 6th month after the onset of diseases.The rehabilitation group surpassed the control group significantly.At the end of 6 months,the average of Barthel Index of rehabilitation group was 83.18?18.22 while that of control group was 63.69?12.73.At the end of 6 months,total cost of rehabilitation group was(56 420.0?17 831.8)Yuan and that of control group was (64 864.2?18 267.5)Yuan.The rehabilitation group had better cost/effectiveness and cost/utility.There was a significant difference of cost between two groups.The total rehabilitation cost of rehabilitation group was 4613 Yuan,accounting for 15.3%of all the cost.Conclusion The tertiary rehabilitation treatment for stroke patients has achieved better cost/effectiveness and cost/utility.In China pharmaceutical cost constitutes the major part of the stroke therapy while the rehabilitation cost only a small portion,which is significantly different from the situation in developed countries.

Adult ; Female ; Gas Poisoning ; complications ; Humans ; Male ; Pulmonary Aspergillosis ; complications

Objective To explore the influence of blocking B7/CD28 co-stimulatory pathway by RNA interference on the rejection response in mice heterogeneous heart transplantation and its mecha- nism.Methods siRNA of which sequence specified to CD80 and CD86mRNA was synthesized in vitro respectively and transfected into donor derived myeloid dendritic cells (DCs).The expression levels of CD80 and CD86 mRNA and surface antigen CD80,CD86 were assayed by semi-quantitative reverse transcription polymerase chain reaction and flow cytometry before and after CD80siRNA and CD86siRNA transfection.Seven days prior to heterogeneous heart transplantation in mice,DCs modi- fied by siRNA were transfused into recipients intraveneously (DC interference group).At the same time,group of allograft transplantation,cyclosporine A (CsA)-treated (CsA injected subcutaneously postoperatively,5 mg.kg~(-1)?d~(-1)) group,group of isograft transplantation,and non-interference DC group (transfusion of non-interfered DCs pre-transplanting) were assigned.The graft survivals were individually recorded and the graft rejection grading was pathologically evaluated.Interleukin 2 (IL- 2),interferon?(IFN-?),and IL-10 mRNA expression levels in grafts tissue were determined.Results After siRNA transfected into DC,the expression levels of CD80 and CD86 mRNA were down- regulated significantly and the the antigen CD80~+ and CD86~+ reduced from 84%,67% to 35% and 30% respectively.As compared with groups of allograft and non-interference DC,survival of the grafts was significantly longer in DC interference group (P＜0.01),pathological grade of rejection significantly lower (P＜0.01),IL-2 and IFN-?mRNA expression levels lower,and IL-10 mRNA ex- pression levels higher in grafts tissue (P＜0.01).Conclusion Knocking down the molecule B7 expres- sion level in donor-derived myeloid DCs through RNAi,which could block B7/CD28 co-stimulatory pathway,could exhibit inhibitive effect on rejection response in mice heart transplantation.The mechanism might be due to induction of T lymphocyte anergy and Th cell differentiation deviating to T_H2.

Adult ; Aged ; China ; epidemiology ; Female ; Gold ; Humans ; Male ; Middle Aged ; Mining ; Silicosis ; mortality ; Young Adult

Objective To study the assiociation of-866G/A polymorphism in the promoter of uncoupling protein 2(UCP2)gene in patients with type 2 diabetes and B-cell function in Han population in Nanjing.Methods For the case-control study,PCR-RFLP method was used to determine the distributions of allele and genotype frequencies of-866G/A polymorphism in the human UCP2 gene in 229 type 2 diabetic patients and 196 normal control subjects.All of the testees accepted oral glucose tolerance test and insulin releasing test to detect B-cell secretion function and insulin sensitivity.Results The distribution of genotype and allele frequencies of-866G/A polymorphism in the promoter of the human UCP2 gene were significantly different between type 2 diabetic patients and normal control subjects(?2=6.555,P=0.038;?2=6.363,P=0.012 respectively).The frequency of A/A genotype and A allele in type 2 diabetic were significantly higher than that in contrast(OR=1.99,OR=1.42 respectively,both P

Remarkable achievements have been made for lymphocyte infusion of lymphoma and leukemia, especially of lymphoproliferative disease within twenty-first century. The donor lymphocyte infusion or mobilized donor lymphocyte infusion (DLI/DSI) offer an opportunity of second remission for relapse patients post-transplantation. Cytokine-induced killer/DC cytokine-induced killer play an anti-tumor activity beyond non-MHC restricted. Cytotoxic lymphocyte infusion activated by synthetic tumor antigen produces targeted effects of anti-tumor. Transgenic CTL of anti-tumor TCR has brought the dawn in lymphoma and leukemia patients with defective lymphocytes. Maternal lymphocyte infusion play an anti-tumor/viral activity by avoiding the immune barrier of HLA mismatch.

Objective To study the effects of nicotine on GDNF and dopamine content in striatum of Parkinson's disease (PD) rats.Methods 6-OHDA was stereoscopically injected to right side of the mesencephic ventral tegmental area (VTA) and substantia nigra pars compacta (SNpc). Nicotine of different dosages was intraperitoneally injected into PD rats.With biochemical,immunohistochemical techniques, we observed the changes of GDNF expression and dopamine content in striatum.Results Nicotine was intraperitoneally injected into PD rat models before or after the operation,striatum GDNF expression and DA content had significant improvement as compared with PD group (P

AIM: To investigate the effects of estrogen interference on ab dominal adipose accumulation in C7BL/6J mice. METHODS: Estrogen was administrated to the ovariectomized mice (estrogen-deprived model) and the changes of abdominal adipose weight of the mice in the normal estrous cycle and pro-menopausal were observed. Mice of 3 months old were randomly divided into c ontrol, ovariectomized (OVX), OVX+estrogen 15-days (E d 15 ) and OVX+ estrog en 60-days (E d 60 ) groups. Each group had 10 mice. Mice were raised and sa crificed until 7 months old after ovariectomized. 5 months and 10 months old mic e were divided into control, estrous cycle normal + estrogen 60 days, pro-menop ausal and pro-menopausal+60 days groups. 17?-estradiol time-release pellets ( 0.18 mg/pellet) were given ic in 15 days and 60 days before mice w ere sacrificed. RESULTS: The abdominal adipose weight in OVX gro up increased significantly (P 0.05 ). There was no difference in the abdominal adipo se weight between control and estrous cycle normal + estrogen 60-days groups, a nd between pro-menopausal and pro-menopausal+ estrogen 60-days groups. CONCLUSION: Ovariectomy significantly influences the metabolism of abd ominal adipose that causes adipose accumulation. Estrogen interference, especial ly long-term administration, can attenuate the abdominal adipose accumulation. Exogenous estrogen interference has no effect on abdominal adipose accumulation on cycle normal and pro-menopausal mice.

Objective: To evaluate the clinical value of positron emission tomography/ computed tomography(PET/CT) with fluorine-18-labeled fluorodeoxyglucose(18F-FDG) in dectecting and diagnosing the recurrence and metastasis of colorectal cancer.Methods: The results of whole-body PET/CT of 68 patients with colorectal cancer were analyzed retrospectively.The average follow-up time was 15.8 months.The diagnosis of recurrence tumor and/or metastasis was based on pathologic examination,colonoscopy,multi-modality imaging and clinical follow-up.Results: Fifty-five of the 68 patients had recurrence and /or metastasis but no recurrence was found in the other 13 cases.The sensitivity,specificity and accuracy of PET/CT in detecting recurrent and metastatic tumor were 96.4%,76.9% and 92.6% respectively.PET/CT successfully detected one or several malignant insidious lesions in 8 cases with negative findings by CT and /or ultrasonography,and it revaeled more malignant lesions than CT and ultrasonography in 30.9%(17/55)of the patients.The clinical therapeutic strategies were changed in 11 case duo to the results of PET/CT,and the influence rate of PET/CT was 16.2%.The metastasis was mainly confined in the liver,lung,peritoneal cavity and retroperitoneal region.In the examination of the 24 patients with increased serum CEA,the positive rate of PET/CT was 91.7%.Conlusion: PET/CT can detect the recurrence and metastasis of colorectal cancer with high sensitivity and accuracy.