The incidence of gastric carcinoma is increasing and lymphatic metastasis is one of the major causes of death.Vascular endothelial growth factor-C(VEGF-C),Vascular endothelial growth factor D(VEGF-D)linking their receptor(VEGFR-3)can increase lymphangiogenesis,advance lymphatic metastasis,and relate to lymphatic vessel density of tumor.More and more studies of lymphatic vessel of gastric carcinoma cause great attention.We summarizeed the mechanism of lymphangiogenesis,the new development of relationship between lymphangiogenesis and lymphatic vessel density and their clinical significance.

Objective To explore the situation and the impact of early postnatal sucking and health educa-tion on early maternal lactation physical rehabilitation of primipara.Methods 100 primiparas were randomly divided into two groups,50 cases in each group.The control group received routine care,the observation group was given suck and health care education on the basis of the control group.The maternal lactation and physical rehabilitation was compared between the two groups.Results The good rate of the maternal health knowledge in the observation group was 92.00%,the good rate of ability of nursing master was 86.00%,the lactation time was (16.46 ±2.43)h,which were significantly better than the control group(χ2 =14.03,14.89,t =10.22,all P <0.05).The postpartum rehabil-itations of the observation group were better than those of the control group(t =3.86,4.42,4.68,5.67,677,5.46, 571,4.27,3.98,508,all P <0.05).The incidence rate of complication of the observation group was 6.00%,which was significantly lower than 22.00% of the control group(χ2 =5.24,P <0.05).Conclusion First mothers receive early postnatal sucking and health education can increase their milk production capacity,promote physical rehabilitation.

Objective To observe the therapeutic effect of the integration of Longji-Huoluo pill and mild manipulations Tuina therapy for the teenagers with lumbar intervertebral disc protrusion. Methods Eligible teenager patients with lumber intervertebral disc protrusion were randomly classified into two groups according to the single blind method and the treating sequence, 69 patients in the treatment group and 58 patients in the control group. The Longji-Huoluo pill and mild Tuina manipulations were adopted on the basis of the convertional treatment in the treatment group. While ibuprofen sustained release capsules and mecobalamin were given to the control group beside the convertional treatment. Both groups were treated for 4 weeks. The CT examinations were applied before and after the treatments to observe the absorption of the prolapsed lumber intervertebral disc. The Visual Analogue Scale (VAS) was used to test the pain degree and to estimate the clinical effect. Results The VAS scores in the treatment group (4.03 ± 1.67 vs. 5.41 ± 1.55, t=10.121) and the control group (3.61 ± 1.34 vs. 5.96 ± 1.71, t=11.086) after treatment were significantly lower than those before treatment (P<0.05). The sagittal diameters of the prominence in the treatment group (2.12 ± 1.89 mm vs. 4.62 ± 0.34 mm, t=2.281) after treatmentwas significantly lower than that before treatment (P<0.05). The total effective rate of the treatment group was 87.0%(60/69), and the control group was 72.4%(42/58). Statistical significance was detected on the difference of the two groups (U=1.913, P=0.043). Conclusions The integration of manipulations can effectively relieve the pain and can return the prominent disc. The therapeutic effect is considered equivalent to western medicine.

Abstract: Objective To summary and analyze the epidemic situation of imported malaria and the prevention and control measures implemented during the new crown pneumonia epidemic in Shenzhen, so as to provide reference for the prevention and control of imported malaria under COVID-19 epidemic. Methods　The data on the prevention and control of malaria epidemic in Shenzhen from 2017 to 2021 were collected for analyzing the epidemic situation of malaria, measures taken and the effect of prevention and control with descriptive epidemiological methods. Results　From 2017 to 2021, a total of 104 confirmed malaria cases were reported in Shenzhen, all of which were imported from abroad. 97.1% were imported from Africa, mainly falciparum malaria, accounting for 80.8%. The age of onset was mainly young adults, the age group 20-49 years accounted for 81.7%, and most of the patients were overseas migrant workers, accounting for 59.6%. Imported malaria cases were reported in each month, the most was 13 cases in July, then 12 cases in September. From 2017 to 2021, 709 mosquito trap lamps were placed, and 3 523 mosquitoes were captured, with an average mosquito density of 2.60 per lamp and night. Anopheles were not found, and the dominant species were Culex quinquefasciatus. During the outbreak of COVID-19, Shenzhen has implemented a series of measures in terms of improving the working mechanism of multi-departmental cooperation and joint defense, strengthening screening and monitoring, personnel training, mosquito vector control, and material security, so as to achieve early detection, early transfer, early isolation and early treatment. Conclusions　A series of measures have been comprehensively implemented to prevent and control imported malaria in Shenzhen while preventing and controlling imported new coronary pneumonia, and achieved positive results. It has realized the timely identification of cases and effective treatment, and prevented the occurrence of severe and fatal malaria cases, as well as halting the spread and spread of malaria outbreaks.

Child ; Chromobacterium ; Female ; Humans ; Sepsis ; drug therapy ; etiology ; microbiology ; mortality

To investigate the kinetic expression of cytokine in allografts in rats and the effect of hydrocamptothecin on such expression, syngenic and allogenic heart transplantation models were developed. Recipient rats were divided into 3 groups: group A, SD-SD; group B, Wistar-SD group C,Wistar-SD plus treatment with HPCT 2. Omg/(kg ?d). The grafts and spleens were harvested on days 1, 3, 5, 7, and 14 post-transplantation for morphology and RT-PCR detecting the expressions of IL-2, IFN-?, IL-4, IL-10 mRNA. The results showed that the cytokine generally increased commensurately with the infiltration of this leukocyte population. The cytokine mRNA transcript could be detected on days 1 and 3 after transplantation in group A. The IL-2 and IFN-? mRNA transcript could be detected on days 1, 3, 5, 7 and 14 with a peak on day 7 in group B, but on day 3 in group C. The IL-4 and IL-10 mRNA transcript could be detected on days 1,3,5,7 and 14 with a peak on day 5 in group B, while on day 7 in group C. The levels of IL-2 IFN-? mRNA expression were significantly lower in grafts of group C than those in grafts of group B, whereas the levels of IL-4 and IL-10 mRNA were significantly higer in grafts of group C than those in grafts of group B. The level of cytokine mRNA expression in the grafts was similar to that of spleen of recipients. Our conculssion is the expression of cytokine mRNA in allografts can be detected on day 1 after transplantation ,with peaking of IL-2 and IFN-? on day 7, IL-4 and IL-10 on day 5. The survival time of allograft can be prolonged by HCPT. The levels of IL-2 and IFN-? mRNA expression can be reduced, while the levels of IL-4 and IL-10 mRNA expression be enhanced by HCPT.

Objective To construct the lentivirus plasmids of human Toll like receptor-5(TLR5)and nuclear factor(NF)-κB signaling pathway responsive luciferase and green fluorescent protein(GFP)reporters, and establish a HEK-293 cell line stably expressing human TLR5 and NF-κB responsive reporters. Methods The TLR5 and 5×NF-κB-binding sites genes were cloned to lentiviral expressing plasmid pWSLV-puro and pWSLV-nanluc-GFP, respectively. The recombinant plasmids, pWSLV-hTLR5-puro and pWSLV-5×NF-κB-nanluc-GFP were respectively transfected with packaging plasmid into HEK293T cells in order to package lentivirus. The lentivirus was used to coinfect HEK-293 cells, and the positive clones stably expressing TLR5 and NF-κB responsive reporter (HEK293-N-T)were generated using colony-purification in present of puromycin and fluorescence activated cell sorting. The cells expressing TLR5 were confirmed by Western blot and immunofluorescence. Additionally, the cells expressing NF-κB responsive reporter were confirmed by fluorescence microscopy and luciferase quantitative assay. Results The results of recombinant plasmids digestion identification and the sequencing of cDNA showed that the sequences inserted into the plasmid and the expected sequences of hTLR5 and 5×NF-κB-binding sites genes were completely consistent. It was confirmed that the hTLR5 and 5×NF-κB-binding sites genes were correctly inserted into the vectors, and that the recombinant plasmids, pWSLV-hTLR5-puro and pWSLV-5×NF-κB-nanluc-GFP, were successfully constructed. Additionally, Western blot and immunofluorescence analysis showed that TLR5 were stably expressed in a high level in the HEK293-N-T cells and located in the cell membranes. Moreover, the results of fluorescence microscopy and luciferase quantitative assay indicated that the NF-κB responsive reporter could reflect the activation of NF-κB signaling in a dose-dependent manner. Conclusion The HEK-293 cell line stably expressing human TLR5 and NF-κB responsive reporter has been successfully established and the HEK293-N-T cells can be used for the screening and evaluation of drugs target to TLR5.

Objective To introduce the Chinese version of short Falls Efficacy Scale International (short FES-I) and test its reliability and validity in patients with cerebral infarction. Methods The English version and the traditional Chinese version of short FES-I were ob-tained from the Prevention of Falls Network Europe, and the simplified Chinese version was developed after a further revision. A sample of 105 inpatients with cerebral infarction from December 2014 to May 2015 were recruited from 2 tertiary hospitals in Tianjin. They were in-vestigated with the simplified Chinese version of short FES-I, modified Falls Efficacy Scale (MFES), Generalized Anxiety Disorder-7 (GAD-7) and Patient Health Questionnaire-9 (PHQ-9). At the same time, the laboratory indexes of the patients were collected. Results The Cronbach'sαcoefficient of short FES-I was 0.98 and the score of short FES-I was negatively correlated with MFES (r=-0.41, P<0.001). Short FES-I was correlated wtih GAD-7 score (r=0.52, P<0.001), PHQ-9 score (r=0.46, P<0.001) and the level of C reaction protein (r=0.21, P=0.032), but uncorrelated with other laboratory indexes (P>0.05). There was significant difference in the score of short FES-I be-tween the patients with or without falling. Conclusion Short FES-I is valid and internal consistant, and can be used as an assessment tool to screen fear of falling among patients with cerebral infarction.

Objective To investigate the molecular mechanism of Helicobacter pylori (H.pylori) resistance to clarithromycin. Methods The E test was used to determine clarithromycin resistant strains of H.pylori , and PCR Restriction Fragment Length Polymorphism (RFLP) analysis for 23S rRNA domain V gene mutations. Results Of nine clarithromycin resistant stains of H.pylori , including six primary and three acquired resistant strains, eight were found to have an A to G mutation in 23S rRNA domain V at position 2144. Conclusions The results indicated that the majority (88.8%) of clarithromycin resistant isolates of H.pylori in Shanghai have an A2144G mutation in 23S rRNA domain V.

Objective To compare the effects of oral Sildenafil(SIL)and intravenous prostaglandin E_1(Alprostadil,PGE_1)in treatment of postoperative pulmonary hypertension(PH)associated with congenital heart defects(CHD).Methods Data were collected from 24 children with postoperative mPAP≥35 mmHg in our hospital between August 2004 and March 2005.These children were randomly divided into three groups:8 children(group A)initially received SIL(0.35 mg/kg,orally by nasal gastric tube),followed by the addition of intravenous PGE_1(20 ng/kg per min)at 40 min.The second group of 8 children(group C)initially received intravenous PGE_1 followed by the addition of SIL at 20 min(dose as above).And remains(group B)were placebo-controlled.The changes of hemodynamic variables,arterial blood gas,lung static compliance(C)and work of breathing(W)were measured after drugs were given.Results Compared with group B,the mPAP and mPAP/mSAP was significantly reduced(P