Objective To explore the effect of treadmill exercises on disuse osteoporosis and the media effect of OPG-RANKL-RANK system of osteoclast differentiation in the process.Methods Forty six-week-old male Sprague-Dawley rats were randomly divided into a normal control group,a disuse model group,a normal recovery group and an exercise recovery group,each of 10.The normal control group were sacrificed 4 weeks later without receiving any special treatment.The disuse model group were sacrificed after 4 weeks of tail suspension.The normal recovery group were sacrificed after 4 weeks of tail suspension and keeping quiet for 4 weeks.The exercise recovery group were sacrificed after 4 weeks of tail suspension and another 4 weeks of treadmill exercises.The indicators of bone mineral density (BMD),bone histomorphometry,bone tissue TRACP-5b staining,bone metabolism and cytokines related to the osteoclast differentiation OPG-RANKL-RANK system were tested immediately after the rats were sacrificed.Results The BMD,trabecular bone volume percentage (TBV),trabecular bone width (Tb.Wi),concentration of serum alkaline phosphatase (ALP) and the gene expression of bone marrow oculopneumoplethysmograph (OPG) of the disuse model goup were significantly lower than those of the normal control group (P＜0.01),while the trabecular bone spacing (Tb.Sp),average positive staining area percentage (APSAP) of tartrate-resistant acid phosphatase (TRACP)-5b,concentration of serum Ca2+ and TRACP-5b and the gene expression of bone marrow cytokines including RANKL,macrophage colony-stinulating factor (M-CSF),RANK,interleukin (IL)-6 and tumor necrosis factor (TNF)-α of the disuse model goup were significantly higher than those of the normal control group (P＜0.01).The BMD,TBV,Tb.Wi,concentration of serum ALP and the gene expression of bone marrow OPG of the exercise recovery group were significantly higher than those of the normal recovery group (P＜0.05 or P＜0.01).The Tb.Sp,APSAP,concentration of serum Ca2+ and TRACP-5b and the gene expression of RANKL,M-CSF,RANK,IL-6 and TNF-α of the exercise recovery group were significantly lower than those of the normal recovery group (P＜0.05 or P＜0.01).Conclusion The 4-week tail suspension can lead to disuse osteoporosis in rats.Treadmill exercise can promote the recovery of rats with the disuse osteoporosis.The occurrence of disuse osteoporosis and the effect of exercises on disuse osteoporosis were related with the expressing of OPG-RANKL-RANK system-related cytokines of osteoclast differentiation in bone marrow microenvironment.

Purpose　To improve the diagnostic level of liver and spleen traumatic rupture in CT. Methods　39 cases of liver and spleen traumatic rupture diagnosed with CT images,analyzed comparing with CT findings,surgical outcome and clinical representation. Rusults　There are two categories of CT findings in cases of liver and spleen traumatic rupture. Liver and spleen traumatic avulsion: 7cases represented high density patches in liver and spleen parenchyma; 4cases represented low density zonary focus;18 cases represented irregular low density patches. 11 cases represented multi-spots of high density in above mentioned focuses. Hematoma under envelop of liver and spleen: 15 cases represented same or low density lunular focuses lying the edge of liver and spleen, some fresh hematoma represented high density. Conclusion　For diagnosing the liver and spleen traumatic rupture, following CT findings are valuable: ①Irregular high and low density bleeding focuses in liver and spleen; ②Lunular low density hematoma under envelop of liver; ③ Celiac hematocele symptom.

Objective To investigate the effects of Additive Foshousan(AFSS) on the serum levels of estradiol and progesterone in rats with primary dysmenorrhea. Methods SD rats were divided into six groups, each 10. Group 1 was normal control , groups 2,3 and 4 were treated with low, middle and high dose of AFSS(0.75, 1.5 and 3.0 g/kg) respectively, group 5 as positive controls was treated with Yuanhuzhitong tablets (1 mg/kg)and group 6 served as model control. Except the normal control group, all rats were injected diethylstilbestrol and oxytocin to establish primary dysmenorrhea model. The levels of estradiol(E2)and progesterone(P) in rats serum was determined by ELISA method and the ratio of E2/P was calculated. Results In middle and high dose of AFSS groups, the level of E2(48.27±6.42)pg/L,(47.51±7.03)pg/L respectively were lower than that in model group(54.47±9.12)pg/L and the difference was statistically significant(P<0.05). In low dose of AFSS group, the level of E2 was(50.83±6.26)pg/L and the difference was no statistically significant compared with model group. In all doses of AFSS groups,The content of P(687.41±21.14)ng/L, (720.47±41.03)ng/L, (719.78±32.01)ng/L respectively were higher than that in the model control group (667.32±46.51)ng/L and the difference was statistically significant(P<0.05 or<0.01). In middle and high dose of AFSS groups, the content of P were higher than that of Yuanhu-Zhitong tablets group(699.31±36.31)and the difference was statistically significant(P<0.05). Conclusion To reduce the content of E2, increase of P the content and decrease ratio of E2/P is one of the mechanism for AFSS to treat primary dysmenorrhea.

Objective:To establish an improved method for the determination of diethylene glycol and ethylene glycol in glycerol on the basis of the method in Chinese pharmacopoeia (2010 edition). Methods: Glycerol samples were extracted by methanol, and detected by gas chromatography with a flame ionization detector. The column was a DM-624 capillary column(30 m × 0. 53 mm,3μm), and the initial temperature was 80℃ in the first 6min and then risen to 100℃ at the speed of 50℃·min-1, kept for 8min,fi-nally risen to 220℃ at the speed of 10℃·min-1 , kept for 10min. The split injection was used with the split ratio of 100∶1. The in-jection temperature was 250℃ and the detector temperature was 280℃. The flow rate was 6. 0ml·min-1 . Results:The RSD(%) of the method was lower than 5%, and the resolution of all chromatographic peaks met the requirements of the pharmacopoeia. The meth-od was used to analyze the commercial product of glycerol, and the contents of diethylene glycol and ethylene glycol were both less than the specified limits. Conclusion:The method is simple and rapid, and suitable for glycerol impurity inspection.

Objective To analyze the clinical features of vasovagal syncope, enhance its clinial awareness and to promote the importance of history taking in the differential diagnosis of syncope. Methods A total of 83 patients with the diagnosis of vasovagal syncope were included in the study and their data were, undergone retrospective statistical analysis. Results A 63.9% of patients had recurrent syncope. There was 73.5% of them had motivation and 80.7% of them had presyncope symptoms. Complete loss of consciousness was found in 77.1% of patients. There was 39.1% of them had concomitant symptoms and 39.1% had postsyncopal symptoms. In 54.2% of the patients the duration of syncope was less than 2 minutes. Conclusion It is important to pay attention to history taking and to anylaze the clinical features in the diagnosis of vasovagal syncope.

Objective To study the relationship of ampicillin resistance and?lactamase production, and gene mutation on pbp5 gene fragment in Enterococcus faecium. Methods?lactamase production was tested with nitrocefin, the pbp5 gene fragment of 57 isolates of ampicillin-resistant Enterococcus faecium (ARE. faecium) was amplified by PCR, the mutation of amplified pbp5 gene was detected by single-strand conformation polymorphism(SSCP), the specific mutation types were confirmed by sequencing. Results?lactamase was negative in 57 isolates of ARE. faeciums; there were 3-6 gene mutation sites on pbp5 gene fragment, and the frequent mutation sites were the position 485 ,499 and 466'. Conclusion The gene mutation of pbp5 gene fragment may play an important role in the ARE. faecium.

Objective To observe the efficacy of arterial thrombolysis treatment in patients with acute cerebral infarction caused by internal carotid artery(ICA),middle cerebral artery(MCA)and vertebrobasilar artery(VBA)occlusion and during different time windows.Methods In the 40 patients with different artery(12 ICA,18 MCA and 10 VBA)occlusions,19 patients with the time windows of 0.05).The rate of better prognosis at 90 d followed up with MRS demonstrated 77.8% in MCA,41.7% in ICA and 30.0% in VBA and there were significant difference among these three groups(all P

Cancer stem cells (CSC) are related to the progression,recurrence and drug-resistance of tumor.MicroRNAs have been reported to correlate to the self-renewal of cancer cells.They can regulate cell cycle and thus interfere with the cell fate.Recent publications have clafified that microRNA can effect on cancer stem cells' self-renewal either by regulate targeted genes directly,or through wnt,notch and Hedgehog pathways.

Objective To investigate effect of butylphthalide on serum sex hormone,5-HT and sleep quality in patients with stroke sleep disorder. Methods 240 patients with stroke sleep disorder in our hospital were selected,according to clinical medication were divided into 2 groups.Control group (n=120) were treated by routine treatment, experimental group (n=120) was treated on the base of the control group with butylphthalide.Serum sex hormones, 5-HT and PSQI levels were detected after the treatment.Results Compared with the control group,LH, FSH levels of the experiment were higher(P<0.05),E2 level was lower(P<0.05),serum 5-TH level was higher(P<0.05),PSQI score was lower(P<0.05),the difference was statistically significant.Conclusion Butylphthalide can improve stroke sleep disorder serum sex hormone and 5-HT levels, improve sleep quality, has guiding significance for the treatment.

The success of yielding induced pluripotent stem (iPS) cells from human somatic cells demonstrates the important role of reprogramming in the formation of stem/progenitor cells and initiates the exploration of the origin of leukemia stem cells. In our previous work, we have found two types of leukemia, bona fide leukemia and non-bona fide leukemia. Different leukemias originate from different leukemia stem/progenitor cells which are critical to the genesis and evolution of leukemia. Bona fide leukemia and non-bona fide leukemia originate from leukemia stem cells and progenitor cells, respectively. Recent research suggests that different types of leukemia are influenced by the reprogramming state of their origin cells.
Cell Differentiation ; Cellular Reprogramming ; Humans ; Induced Pluripotent Stem Cells ; Leukemia ; genetics ; Neoplastic Stem Cells ; Stem Cells