Metabolism of n-alkane mainly includes ?-oxidation pathway and ?-oxidation pathway in Candida tropicalis,which are both catalyzed by a series of enzymes. The transformation from n-alkane to fatty acid is accomplished via ?-oxidation pathway,and the degradation of fatty acid is involved in ?-oxidation pathway. The mechanism on metabolism of n-alkane and studies on related enzymes are summarized in the review. Furthermore,some molecular genetics and metabolic engineering techniques related to Candida tropicalis developed in recent years are introduced.

Transcatheter electrical ablation was performed in 5 patients, none of them showed clinical effectiveness for treatment of antiarrhythmic drugs, with recurrent sustained ventricular tachycardia (VT). The localization of ventricular ectopic focus mainly depended upon the results of pace mapping. Energy used for each transcatheter electrical ablation (TEA) was usually 150-250J and the total energy was 550J to 800J respectively for each patient. No recurrence of VT was ob-served in 4 cases during 19 to 41 (mean 26. 8) months of follow-up period, two of them without adminstration of drug. Sustained VT relapsed during the 4th months after ablation in one case, however, heart rate during VT was much slower than before. Our experience suggests that long-term effectiveness of TEA for treatment of drug-resist VT is inspiring.

Objective: To discuss the values of serum S100 protein in evaluating the cerebral injuries during and after cardiopulmonary bypass (CPB). Methods: 40 patients (25 valve replacement, 13 simple congenital heart disease, 2 valve replacement plus CABG) were studied. Serum S100 protein was serially assessed at different time intervals and the patients' neuropyscological complications were recorded. Results: S100 was not detected before CPB, and it reached its peak level at the termination of CPB, then gradually decrease to the preoperative levels. Three patients with the highest level appeared neuropyscological mobidity. Conclusion: S100 protein has significant change during CPB, and may reflect both the severity of the cerebral injury and increased permeability of the blood brain barrier. Its level has very great significance in evaluating cerebral injuries.

Objective To evaluate the value of ultrasonography in the diagnosis of special ectopic pregnancy(SEP).Methods Ultrasonography was performed in 31 patients with SEP.The sonographic features of two-dimensional ultrasound and color Doppler of SEP were analyzed and the sonographic results were correlated with surgical and pathologic findings. Results Ultrasonography accurately diagnosed interstital tubal pregnancy in 21 cases,cervical pregnancy in 4,isthmus pregnancy in 2,rudimentary horn pregnancy in 1,and 3 cases were misdiagnosed,thus,the diagnostic accuracy of ultrasound for SPE was 90.3% .Conclusions Ultrasonography has an important role in diagnosing SEP.

[Objective] To observe the inhibiting capacity of Bingcha Shuan Containing serum to HEPG2 cells' reproductive activity and to conjecture.Bingcha Shuan has the potential to cure the liver carcinoma.[Method]According to serum pharmacology,HEPG2 cells were cultured in vitro and interfered by various concentrations of Bingcha Shuan contained serum for 24,48 hours respectively.The inhibiting capacity of the Bingcha Shuan contained Serum to HEPG2 cells were detected by MTT.[Result] The Bingcha Shuan contained Serum has the inhibiting capacity to HEPG2 cells in some degree.And there was a marked positive correlation between drug concentration,time and inhibition ratio.[Conclusion] It is suggested that Bingcha Shuan has the inhibiting capacity of cell proliferation and it can provide the support on how to cure the liver cancer of clinic.

Based on the literatures in MEDLINE database and EMBASE databases this article has carried out a bibliometrics study on foreign clinical researching institutions of acupuncture and moxibustion to screen those institutions who had more published articles as our major cooperation and exchange objects in the future,hoping for providing information basis of TCM international scientific research.

Objective To explore the clinical characters and the relationship between prognosis and treatment in childhood acute non-lymphocytic leukemia(ANLL).Method We summarized and analyzed the cases with acute non-lymphocytic leukemia in children patients from our hospital admission between Jan 2003 and Sep 2007.Results ①The M1,M2 subtype of childhood ANLL were about 37.1%;the clinical situation was various;extramedullary infiltration was not uncommon;②A totle complete remission(CR)rate was 68.6% and the CR rate of one course chemotherapy was 40.1%;③The CR rate of acute promyelocytic leukemia(APL)was 87.5% and a average of continues CR(CCR)was 33 months after the treatment of ATRA combined with As2O3;④Among five patients finished all the courses of high dose Ara-c(HDAra-c)intensification therapy,three were CCR over 36 months.Conclusion ①The CR rate of one course chemotherapy was correlated to the intensity of treatment;②The treatment of As2O3 can help to lower the relapse of APL and enhance the event-free survival(EFS);③The treatment of HDAra-c can help to increase the EFS rate of childhood ANLL.

Diagnostic Errors ; Female ; Femoral Neck Fractures ; diagnosis ; diagnostic imaging ; Femur Neck ; diagnostic imaging ; injuries ; Humans ; Radiography ; Young Adult

BACKGROUND: Schwann calls are seed cells for constructing tissue engineered peripheral nerves. But their in vitro isolation, culture and purification are difficult. Acellular nerve allografts (ANA) have a great effect on repairing peripheral nerve defects, and it can induce marrow stromal cells (MSCs) into Schwann-like calls. Accordingly, MSCs can be used as seed calls theoretically in constructing tissue engineered peripheral nerves, instead of Schwann cells OBJECTIVE: To observe the repairing effect of tissue engineered peripheral nerves constructed with MSCs on sciatic nerve defects and to assess the feasibility of repairing peripheral nerve defects with MSCs as seed calls. DESIGN, TIME AND SE'I-rlNG: A randomized control animal experiment was conducted in the laboratory of Basic Medicine Collage of Dali Collage from July to December in 2008.MATERIALS: A total of 30 SD rats were divided randomly into 3 groups, with 10 in each group. In MSCs+ ANA group, end-to-end anastomosis were performed with 10/0 non traumatic suture to broken ends of rat sciatic nerves and tissue engineered nerves cultured using MSCs combined with ANA; In ANA group, ANA were bridged to broken ends of sciatic nerves; In METHODS: The 10ram sciatic nerve defects in rats were repaired using MSCs-constructed tissue engineered peripheral nerves, whose repairing effects were evaluated at week 12 post transplant through sciatic function index (SFI), gastrocnemius wet weight recovery rate, S-100 immunohistochemical staining and electron microscope observation, etc. MAIN OUTCOME MEASURES: Morphological changes of calls were observed during the culture of compounds; SFI and gastrocnemius wet weight recovery rates were detected after transplantation; New myelinization, axon growth and nerve fiber distdbuUon were observed with toluidine blue staining; Schwann calls growth and nerve fiber regeneration were observed with transmission electron microscope combined with S-100 protein immunohistochemical staining method.RESULTS: The detection results showed that SFI and gastrocnemius wet weight recovery rate were higher in the MSCs+ ANA group than in the ANA group (P < 0.05). Compared the ANA group, the MSCs+ ANA group had more S-100 proteins expressed in its compounds obviously, and the number, the diameter of its myelinated nerve fibers as well as its myelin sheath thickness were all greater than those of the ANA group (P < 0.05). The repairing effect of the MSCs+ ANA group was close to that of the autotransplantation group.CONCLUSION: MSCs-constructed tissue engineered nerves have a better effect on repairing peripheral nerve defects than ANA, and MSCs as seed cells have a high value in peripheral nerve tissue engineering.