Metabolism of n-alkane mainly includes ?-oxidation pathway and ?-oxidation pathway in Candida tropicalis,which are both catalyzed by a series of enzymes. The transformation from n-alkane to fatty acid is accomplished via ?-oxidation pathway,and the degradation of fatty acid is involved in ?-oxidation pathway. The mechanism on metabolism of n-alkane and studies on related enzymes are summarized in the review. Furthermore,some molecular genetics and metabolic engineering techniques related to Candida tropicalis developed in recent years are introduced.

Based on the literatures in MEDLINE database and EMBASE databases this article has carried out a bibliometrics study on foreign clinical researching institutions of acupuncture and moxibustion to screen those institutions who had more published articles as our major cooperation and exchange objects in the future,hoping for providing information basis of TCM international scientific research.

Transcatheter electrical ablation was performed in 5 patients, none of them showed clinical effectiveness for treatment of antiarrhythmic drugs, with recurrent sustained ventricular tachycardia (VT). The localization of ventricular ectopic focus mainly depended upon the results of pace mapping. Energy used for each transcatheter electrical ablation (TEA) was usually 150-250J and the total energy was 550J to 800J respectively for each patient. No recurrence of VT was ob-served in 4 cases during 19 to 41 (mean 26. 8) months of follow-up period, two of them without adminstration of drug. Sustained VT relapsed during the 4th months after ablation in one case, however, heart rate during VT was much slower than before. Our experience suggests that long-term effectiveness of TEA for treatment of drug-resist VT is inspiring.

[Objective] To observe the inhibiting capacity of Bingcha Shuan Containing serum to HEPG2 cells' reproductive activity and to conjecture.Bingcha Shuan has the potential to cure the liver carcinoma.[Method]According to serum pharmacology,HEPG2 cells were cultured in vitro and interfered by various concentrations of Bingcha Shuan contained serum for 24,48 hours respectively.The inhibiting capacity of the Bingcha Shuan contained Serum to HEPG2 cells were detected by MTT.[Result] The Bingcha Shuan contained Serum has the inhibiting capacity to HEPG2 cells in some degree.And there was a marked positive correlation between drug concentration,time and inhibition ratio.[Conclusion] It is suggested that Bingcha Shuan has the inhibiting capacity of cell proliferation and it can provide the support on how to cure the liver cancer of clinic.

Objective: To discuss the values of serum S100 protein in evaluating the cerebral injuries during and after cardiopulmonary bypass (CPB). Methods: 40 patients (25 valve replacement, 13 simple congenital heart disease, 2 valve replacement plus CABG) were studied. Serum S100 protein was serially assessed at different time intervals and the patients' neuropyscological complications were recorded. Results: S100 was not detected before CPB, and it reached its peak level at the termination of CPB, then gradually decrease to the preoperative levels. Three patients with the highest level appeared neuropyscological mobidity. Conclusion: S100 protein has significant change during CPB, and may reflect both the severity of the cerebral injury and increased permeability of the blood brain barrier. Its level has very great significance in evaluating cerebral injuries.

Objective To explore the clinical characters and the relationship between prognosis and treatment in childhood acute non-lymphocytic leukemia(ANLL).Method We summarized and analyzed the cases with acute non-lymphocytic leukemia in children patients from our hospital admission between Jan 2003 and Sep 2007.Results ①The M1,M2 subtype of childhood ANLL were about 37.1%;the clinical situation was various;extramedullary infiltration was not uncommon;②A totle complete remission(CR)rate was 68.6% and the CR rate of one course chemotherapy was 40.1%;③The CR rate of acute promyelocytic leukemia(APL)was 87.5% and a average of continues CR(CCR)was 33 months after the treatment of ATRA combined with As2O3;④Among five patients finished all the courses of high dose Ara-c(HDAra-c)intensification therapy,three were CCR over 36 months.Conclusion ①The CR rate of one course chemotherapy was correlated to the intensity of treatment;②The treatment of As2O3 can help to lower the relapse of APL and enhance the event-free survival(EFS);③The treatment of HDAra-c can help to increase the EFS rate of childhood ANLL.

Objective To evaluate the value of ultrasonography in the diagnosis of special ectopic pregnancy(SEP).Methods Ultrasonography was performed in 31 patients with SEP.The sonographic features of two-dimensional ultrasound and color Doppler of SEP were analyzed and the sonographic results were correlated with surgical and pathologic findings. Results Ultrasonography accurately diagnosed interstital tubal pregnancy in 21 cases,cervical pregnancy in 4,isthmus pregnancy in 2,rudimentary horn pregnancy in 1,and 3 cases were misdiagnosed,thus,the diagnostic accuracy of ultrasound for SPE was 90.3% .Conclusions Ultrasonography has an important role in diagnosing SEP.

Objective To investigate the retainage rate,distribution,and emigration of the bone marrow mesenchymal stem cell(BMMSC) after transcoronary infusion and to further evaluate the feasibility of injecting BMMSCs into coronary artery.Methods BMMSCs were isolated,purified,expanded,and labelled with CM-DiI.The infarcted SD rat hearts were removed and perfused with Langendorff apparatus.The cells were injected into the aortic root and the fluid returning from the coronary system was collected and the labelled cells in the coronary effluent were quantitated with flowcytometry.At the same time,left ventricle function was recorded to evaluate the safety of this approach.In vivo study,the cells were then injected into clamped ascending aorta through a catheter inserted through the left ventricle into the aortic root.The hearts were harvested at different time points after cell transplantation to obtain the direct evidence of distribution and emigration of the implanted cells.Results Only 3%～5% of transplanted cells returned into the right ventricle and more than 90% cells retained in the heart after beinginjected into aortic root of Langendorff model of infarcted hearts of SD rats.Left ventricle function did not deteriorate after cell transplantation.The labelled cells were entrapped within the coronary capillary immediately after cell infusion,mainly in the normal area.After 24 hours some cells migrated through the capillary wall into interstitium of the heart.One week later we found that most survival cells located at the infarcted area and the border zone.Conclusion The majority of BMMSCs delivered by transcoronary infusion retained in the heart.BMMSCs can penetrate the vessel wall and home back to the interstitial compartment and the injured area in a few hours.

OBJECTIVE To investigate the incidence of nosocomial infection in ICU patients,risk factors.and measures to prevent infection.METHODS The nosocomial infection of ICU patients in the hospitals from Nov 2005 to Jun 2009 was investigated using the method of target monitoring.The nosocomial infection rate was regulated by the method of ASA.The invasive procedure and the associated infection rate were analyzed.RESULTS Among 833 inpatients in ICU,92 suffered from nosocomial infection,The nosocomial infection rate was 11.04%.and the nosocomial infection rate per day was 4.43% after modified by the method of ASA.The incidence of patients was 5.37%.Totally 145 strains of bacteria were isolated,and the dominant pathogens were Gram-negative bacilli(56.55%).CONCLUSIONS The patients in ICU are susceptible population of nosocomial infection,A.baumannii is the dominating pathogen to cause nosocomial infection in intensive care units.Target monitoring in ICU is an effective surveillance method to reduce the prevalence of nosocomial infection.