Adult ; Female ; Gas Poisoning ; complications ; Humans ; Male ; Pulmonary Aspergillosis ; complications

Alcoholism ; therapy ; Humans ; Methanol ; poisoning

Objective The experiment aims at probing the best condition of the isolation and purification of rat islets.Methods The islets were isolated from rat pancreas by hepatopancreatic duct perfusing with collagenase and purified with Ficoll 400 discontinuous density gradient centrifugation.Then the purified islets were subjected to histological staining,electron microscopy and radioimmunoassay for identification of specificity and viability.Results The histological staining revealed that the viability and the purity of the purified islets were above 95％and 85％respectively.Electron microscopy showed that the purified islets were morphologically intact with clear membrane and plenty of secreting granules.Radioimmunoassay demonstrated the secreted insulin concerntration between low-glucose groups and high-glucose groups varied significantly,which verified the good function of the islets.Conclusion Hepatopancreatic duct perfusing with collagenase is a good method for digestion.There aye many factors that influence the quantity and quality of the acquired islets,such as the completed expansion of pancreas,the concentration and viability of collagenase and the digested time,etc.

Air ; analysis ; Chromatography, Gas ; methods ; Methylene Chloride ; analysis ; Workplace

Adult ; Fatigue ; Humans ; Male ; Professional Competence ; Sleep Deprivation

Objective:To explore influence of low high density lipoprotein cholesterol (HDL‐C) level on kidney func‐tion in patients with coronary heart disease (CHD) after percutaneous coronary intervention (PCI) .Methods :A to‐tal of 300 CHD patients ,who were treated and received PCI in our hospital from Feb 2012 to Jun 2014 ,were select‐ed .According to HDL‐C level ,they were divided into normal HDL‐C level group (n=179 ,HDL‐C≥1. 04 mmol/L) and low HDL‐C level group (n= 121 ,HDL‐C< 1.04 mmol/L) .Baseline data were compared between two groups ,and multi‐factor Logistic regression analysis was used to analyze risk factors for kidney injury after PCI .Re‐sults:Compared with normal HDL‐C level group ,there were significant rise in percentages of overweight (30.73%vs .39.67% ) ,smoking history (29.05% vs .41.32% ) ,myocardial infarction history (35.20% vs .46.28% ) ,left ventricular ejection fraction (LVEF) <50% (18.99% vs .24.79% ) ,anemia (16.20% vs .27.27% ) and kidney in‐jury (12. 85% vs .22. 31% ) in low HDL‐C level group , P<0. 05 or <0. 01. Multi‐factor Logistic regression analysis indicated that age ,myocardial infarction history ,anemia ,LVEF<50% ,diabetes mellitus ,dosage of contrast agent and low HDL‐C level were risk factors for kidney injury in CHD patients after PCI (β=0.499~1.171 ,P<0.05 or<0.01) .Conclusion:Low HDL‐C level is a risk factor for renal function injury in CHD patients after PCI .

Objective:Through the introduction of BioImp Vet Body Composition principle and application of the second classroom experimental teaching practice in medical function, analyzer application characteristics of BioImp Vet Body Composition. Methods:We studied the distribution of body fluid and fat content determination of rats using BioImp Vet Body Composition. The device using the complex impedance measurement technique in vivo, within the second 4 k-1000 kHz scope, scanned the 256 frequency bands. We measured body water content (TBW), extracellular fluid (ECF) and intracellular fluid (ICF), in addition to the amount of fat (FFM) and fat mass (FM). Results:This measurement technique is very convenient, reliable and quick data acquisition in our second classroom teaching and laboratory. Conclusion:the measurement of BioImp Vet Body Composition can be used in the field of medical experimental teaching, has the application and characteristics of portable and feasible of the second class in the experimental teaching of Medical Function.

Objective The experiment aims at probing the best condition of the isolation and purification of rat islets. Methods The islets were isolated from rat pancreas by hepatopancreatic duct perfusing with collagenase and purified with Ficoll 400 discontinuous density gradient centrifugation.Then the purified islets were subjected to histological staining,electron microscopy and radioimmunoassay for identification of specificity and viability. Results The histological staining revealed that the viability and the purity of the purified islets were above 95% and 85% respectively.Electron microscopy showed that the purified islets were morphologically intact with clear membrane and plenty of secreting granules.Radioimmunoassay demonstrated the secreted insulin concerntration between low-glucose groups and high-glucose groups varied significantly,which verified the good function of the islets.Conclusion Hepatopancreatic duct perfusing with collagenase is a good method for digestion.There are many factors that influence the quantity and quality of the acquired islets,such as the completed expansion of pancreas,the concentration and viability of collagenase and the digested time,etc.

ObjectiveTo investigate the effect of age on long-term prognosis of patients with traumatic brain injury (TBI).Methods102 inpatients with TBI were evaluated by following-up, using Glasgow Outcome Scale (GOS), Functioning Item of Disability Rating Scale (DRS-F) , Employability Item of Disability Rating Scale (DRS-E) and Quality of Life Index (QLI).ResultsThe average age of 102 cases was 29.7±12.5. There were no significant differences among different groups in GOS and DRS-F (P>0.05), but the level of DRS-E or QLI showed much higher in <27 years old group than those in >45 years old group (P<0.01).ConclusionThe factor of age may affect the long-term prognosis of TBI, quality of life and employability of young patients are better than elder patients.

Objective To study the damage focused ultrasound inflicts on the membrane permeability of Ehrlich ascitic tumor (EAC) cells and the relationship between changes in membrane permeability and focused ultra-sound exposure time. Methods The relative survival rate of tumor cells was examined at various intensities and dif-ferent exposure times using focused 2.2 MHz ultrasound. The uhrastructure changes were evaluated with a scanning electron microscope after different exposures. Membrane permeability was investigated by incorporating fluorescein isothiocyanate dextran (FD5OO) , and membrane damage was evaluated by measuring lactate dehydrogenase (LDH) release. Results Morphological observation showed there were numerous microvilli on the surface of un-exposed cells. When the cells had been irradiated with focused ultrasound for 30 s there was only a slight effect on the shape of the cells and the number of microvilli was slightly reduced. When the cells were exposed to ultrasound for 60 s, the surface of many cells became relatively smooth with no obvious microvilli, and several small craters were seen on the surfaces of cells where the cytoplasm seemed to have extruded through the membrane. The cell membrane was seri-ously damaged by sonoporation. The loading of FD500 in the unexposed cells was only 0.21%. When the cells had been sonicated with focused ultrasound for 30 s or 60 s, the loading of FD500 increased to 11.46% and 18.50% re-spectively. The released LDH activities in the 30 s group and 60 s group were 2.94±0.02 and 3.28±0.04 U/L, respectively. The activities of LDH increaased as the focused ultrasound exposure time was prolonged. Conclusion Focused ultrasound may damage the cell membrane permeability of EAC cells, and the damage increases as the expo-sure time is prolonged from 30 s to 60 s.