Animals ; Arterial Occlusive Diseases ; physiopathology ; therapy ; Dogs ; Genetic Therapy ; Hepatocyte Growth Factor ; genetics ; Hindlimb ; blood supply ; Ischemia ; physiopathology ; therapy ; Male ; Plasmids

Objective To investigate the vowel's nasalance and contact quotient (CQ) in children with hearing loss. Methods 57 subjects were divided to 3 groups: profound hearing loss children with hearing aids (HA, n=19), profound hearing loss children with cochlear implant (CI, n=19) and children without hearing loss (n=19). With the help of NV and electroglottography (EGG), the subjects' nasalance and CQ were recorded when they said /a/, /æ/, /i/, /o/, /u/. Results There was no significant differences among the 3 groups on nasalance (P>0.05). There was significant difference among 5 vowels on nasalance, ranked as /u/, /o/, /æ/, /i/, and /a/ (P<0.001). There was significant difference among 3 groups on CQ, ranked as HA, normal, and CI group (P<0.001). There was significant difference on CQ among 5 vowels,ranked as /i/, /u/, /æ/, /o/, and /a/ (P<0.001). Conclusion The voice and resonance should be assessed before speech therapy, which the speech therapy starts from the most correct vowel for children with profound hearing loss.

Acute-On-Chronic Liver Failure ; complications ; virology ; Adult ; DNA Mutational Analysis ; Female ; Genetic Variation ; Hepatitis B virus ; genetics ; Hepatitis B, Chronic ; complications ; virology ; Humans ; Male ; Middle Aged

Hip dislocation represents 2 to 5% of all joint dislocation whereas bilateral hip dislocation occurring as a result of trauma is rarely reported, accounting for 0.025 to 0.050% of hip dislocation cases. It is known that the simultaneous occurrence of traumatic dislocation with one anterior and the other posterior is even rarer. The case described showed a bilateral hip dislocation which occurred anteriorly at the right hip and posteriorly at the left with both femoral heads fractured as a result of a traffic accident. The right hip of the patient was dislocated in the anteroinferior direction of the right acetabulum, and the left hip in the posterosuperior direction of the left acetabulum. Both hip fractures were accompanied by fragments above the fovea centralis. The patient was treated by immediate reduction followed by skin traction. Partial weight bearing was performed after 6 weeks of bed rest and full weight bearing began on the 10th week. No complications such as avascular femoral head necrosis or traumatic arthritis occured.
Accidents, Traffic ; Acetabulum ; Arthritis ; Bed Rest ; Dislocations* ; Fovea Centralis ; Head* ; Hip ; Hip Dislocation ; Hip Fractures ; Humans ; Joints ; Necrosis ; Skin ; Traction ; Weight-Bearing

BACKGROUNDHepatocyte growth factor (HGF) treats ischemic disease by promoting arteriogenesis, however, its mechanism of action is not known. The notch signaling pathway plays an important role in neovascularization. The relationship between the proliferation and migration ability of artery endothelial cells and the Dll4-Notch-Hey2 signaling pathway in the process of arteriogenesis was investigated as a mechanism of action of HGF.

METHODSBased on the prophase study cells and supernatant were harvested at the indicated time after human femoral artery endothelial cells (HFAECs) were infected with adenovirus-HGF (Ad-HGF) at 200 pfu/cell. Cells were analyzed for HGF expression and Notch1, Dll4 and Hey2 expression by ELISA and reverse transcription-PCR (RT-PCR). The changes in the proliferation and migration ability of HFAECs were observed by MTT and Transwell migration experiments. Ad-GFP-infected HFAECs were used as control.

RESULTSCompared with the control group the Ad-HGF group's HGF expression was not increased with time, and the induction by HGF of Notch1, Dll4 and Hey2 gene transcription was not enhanced with an increase of HGF. The proliferation ability of Ad-HGF-transduced HFAECs was enhanced and their migration ability was also enhanced in the presence of HGF.

CONCLUSIONSThrough activating the Dll4-Notch-Hey2 signaling pathway, HGF indirectly promotes the proliferation and migration ability of cells, so that offspring artery branches are formed.

Basic Helix-Loop-Helix Transcription Factors ; genetics ; metabolism ; Cell Line ; Enzyme-Linked Immunosorbent Assay ; Hepatocyte Growth Factor ; genetics ; metabolism ; Humans ; Intercellular Signaling Peptides and Proteins ; genetics ; metabolism ; Receptors, Notch ; genetics ; metabolism ; Repressor Proteins ; genetics ; metabolism ; Reverse Transcriptase Polymerase Chain Reaction ; Signal Transduction ; genetics ; physiology

PURPOSE: The purpose of the current study is to introduce a surgical technique of total hip arthroplasty, by which a postsurgical neurologic deficit can be prevented and excellent clinical and radiological outcome can be obtained in patients with Crowe IV developmental dysplasia of the hip. MATERIALS AND METHODS: Twelve hip joints underwent hip joint arthroplasty at our hospital between October 1995 and November 1997 due to hip joint arthritis secondary to developmental dysplasia of the hip. Among them six hip joints of five patients which belonged to the Crowe Classification IV were operated using cementless total hip arthroplasty with subtrochanteric shortening osteotomy. The medical records and radiographs of these five patients were reviewed. The average follow up period was 61.2 months (range of 40 to 77 months). Preoperative Harris hip score ranged from 61 to 71 (average, 66.2) points. Leg length discrepancy ranged from 2.0 to 5.5 (average, 4.1) cm. RESULTS: Harris Hip Score was improved to 95.3 (87 to 100) points at the last follow-up examination. The postoperative leg length was same bilaterally in five cases except for one case. Bone union in the osteotomy site was obtained three months after surgery. The radiolucent area and findings of movement of the acetabular cup were not observed. Subcidence of the femoral stem or pedestal formation was not observed in five cases. In one case, revision was done for the subcidence of 5 mm or more with radiolucent line around the entire stem and pedestal formation at 48 months postoperatively. One case showed femoral nerve paralysis immediately after the surgery, which recovered completely in six months after the surgery. CONCLUSION: This surgical technique is a useful method in total hip arthroplasty for the Crowe IV developmental dysplasia of the hip without permanent neurologic deficit.
Acetabulum ; Arthritis ; Arthroplasty* ; Arthroplasty, Replacement, Hip* ; Classification ; Crows* ; Femoral Nerve ; Follow-Up Studies ; Hip Joint ; Hip* ; Humans ; Leg ; Medical Records ; Neurologic Manifestations ; Osteotomy* ; Paralysis

Raw or undercooked fish dishes are the major sources of human infection of fishborne trematodes (FBT) and the situation of metacercarial infection in fish greatly affect the prevalence in humans, especially those fish that are commonly used for raw consumption. To investigate the situation of infection with metacercaria of FBT in fish often used to prepare raw fish dishes by local people to assess the risk of infection to humans in Ninh Binh province, Vietnam. 345 fish belonging to five species of freshwater and one species of brackish water fish were collected from fishermen or small-scale fish dealers in Kim Son and Yen Khanh districts, Ninh Binh province between May 2017 and May 2018. Metacercaria of FBT was discovered by pepsin and hydrochloric acid digestion techniques and identified by the morphological and molecular analysis. Among examined fish, 44.06% infected with FBT metacercaria and the highest prevalence was in Cyprinus carpio (86.54%), Ctenopharyngodon idellus (78.43%) and Hypophthalmichthys molitrix (66.67%) while Konosirus punctatus – the brackish water fish – were free from infection. Three species of FBT were found; namely Haplorchis pumilio (accounting for 99.84% of collected metacercariae), Haplorchis taichui and Clonorchis sinensis. The average density was 1.06 metacercariae per gram of freshwater fish and the highest number was of C. idellus (6.38 cysts/gram) followed by Cirrhinus molitorella and C. carpio. Results of the study show the high prevalence of infection of FBT metacercariae among freshwater fish often used to prepare raw fish dishes in Ninh Binh province. These findings suggest the need for greater awareness of the risk from raw fish dishes among public health authorities and people.

We analyzed the practical problems and factors which affect making the correct differential diagnosis in the interpretation or test results from the nocturnal penile tumescence (NPT) test and erotic stimulation test (EST). This was done to provide better information for higher diagnostic accuracy in the clinical application of these tests. The followings are the results of NPT tests and EST identifying the factors affecting correct differential diagnosis by comparison the other differential diagnostic methods. The overall sensitivity of NPT test (Number: 114 total patients) was 82%. 21 cases (18%) could not be diagnosed correctly due to traction of the sensor (12 cases, 10%) and sleep disturbance (9 cases, 8%). The overall sensitivity of EST (Number: 174 total patients) without considering the degree of patient`s sexual drive to erotic stimulation was 77%. 40 cases (23%) could not be diagnosed correctly due to tolerance to pornographic film (17 cases, 10 %), discomfort by the body attachments (14 cases, 8%) and traction of the sensor (9 cases, 5%). However, higher sensitivity (90%) and lower rate of incorrect diagnosis (10% ) were observed in 119 patients who showed Grade II or III (moderate to good) sexual drive to erotic stimulation. The results suggest that undesirable factors in the primary screening methods, traction of sensor, sleep disturbance in NPT test, and tolerance to pornographic film, discomfort by the body attachments, traction of sensor in EST must be taken into consideration when interpretation of test results is being performed.
Diagnosis ; Diagnosis, Differential ; Diagnostic Tests, Routine* ; Erectile Dysfunction* ; Humans ; Male ; Mass Screening ; Penile Erection ; Traction

Vivax malaria reemerged in Korea in 1993 and the outbreak has been continued with fluctuating numbers of annual indigenous cases. Understanding the nature of the genetic population of Plasmodium vivax circulating in Korea is beneficial for the knowledge of the nationwide parasite heterogeneity and in the implementation of malaria control programs in the country. Previously, we analyzed polymorphic nature of merozoite surface protein-1 (MSP-1) and MSP-3α in Korean P. vivax population and identified the Korean P. vivax population has been diversifying rapidly, with the appearance of parasites with new genetic subtypes, despite the recent reduction of the disease incidence. In the present study, we developed simple PCR-RFLP methods for rapid subtyping of MSP-1 and MSP-3α of Korean P. vivax isolates. These PCR-RFLP methods were able to easily distinguish each subtype of Korean P. vivax MSP-1 and MSP-3α with high accuracy. The PCR-RFLP subtyping methods developed here would be easily applied to massive epidemiological studies for molecular surveillance to understand genetic population of P. vivax and to supervise the genetic variation of the parasite circulating in Korea.
Epidemiologic Studies ; Genetic Variation ; Incidence ; Korea ; Malaria ; Malaria, Vivax ; Merozoite Surface Protein 1 ; Parasites ; Plasmodium vivax* ; Plasmodium* ; Population Characteristics

It is very rare for the primary lung cancer to metastasize to gastrointestinal tract, which has poor prognosis. A 72-year-old man admitted with dyspnea and a mass lesion in the left lower lobe on chest roentgenogram and chest computed tomogram(CT). Large cell carcinoma of the lung was diagnosed by percutaneous needle biopsy. Also gastroduodenoscopy was conducted for evaluation of gastrointestinal symptoms, such as indigestion and epigastric discomfort. Large cell carcinoma of duodenum was diagnosed by biopsy of duodenal polyp, which was considered to be metastasized from the primary lung cancer. Palliative radiation therapy was performed, but he died 2 months after diagnosis. We report a rare case of large cell carcinoma of the lung with metastasis to uodenum.
Aged ; Biopsy ; Biopsy, Needle ; Carcinoma, Large Cell* ; Diabetes Mellitus ; Diagnosis ; Duodenum* ; Dyspepsia ; Dyspnea ; Gastrointestinal Tract ; Homocysteine ; Humans ; Lung Neoplasms ; Lung* ; Microcirculation ; Neoplasm Metastasis ; Polyps ; Prognosis ; Thorax