1.A standardized extract of Asparagus officinalis stem prevents reduction in heat shock protein 70 expression in ultraviolet-B-irradiated normal human dermal fibroblasts: an in vitro study.
Ken SHIRATO ; Jun TAKANARI ; Tomoko KODA ; Takuya SAKURAI ; Junetsu OGASAWARA ; Hideki OHNO ; Takako KIZAKI
Environmental Health and Preventive Medicine 2018;23(1):40-40
BACKGROUND:
Heat shock protein 70 (HSP70) exhibits protective effects against ultraviolet (UV)-induced premature skin aging. A standardized extract of Asparagus officinalis stem (EAS) is produced as a novel and unique functional food that induces HSP70 cellular expression. To elucidate the anti-photoaging potencies of EAS, we examined its effects on HSP70 expression levels in UV-B-irradiated normal human dermal fibroblasts (NHDFs).
METHODS:
NHDFs were treated with 1 mg/mL of EAS or dextrin (vehicle control) prior to UV-B irradiation (20 mJ/cm). After culturing NHDFs for different time periods, HSP70 mRNA and protein levels were analyzed using real-time polymerase chain reaction and western blotting, respectively.
RESULTS:
UV-B-irradiated NHDFs showed reduced HSP70 mRNA levels after 1-6 h of culture, which were recovered after 24 h of culture. Treatment with EAS alone for 24 h increased HSP70 mRNA levels in the NHDFs, but the increase was not reflected in its protein levels. On the other hand, pretreatment with EAS abolished the UV-B irradiation-induced reduction in HSP70 expression at both mRNA and protein levels. These results suggest that EAS is capable to preserve HSP70 quantity in UV-B-irradiated NHDFs.
CONCLUSIONS
EAS exhibits anti-photoaging potencies by preventing the reduction in HSP70 expression in UV-irradiated dermal fibroblasts.
Asparagus Plant
;
Cells, Cultured
;
Female
;
Fibroblasts
;
drug effects
;
radiation effects
;
HSP70 Heat-Shock Proteins
;
biosynthesis
;
Humans
;
Middle Aged
;
Plant Extracts
;
pharmacology
;
Polymerase Chain Reaction
;
Skin
;
drug effects
;
radiation effects
;
Skin Aging
;
drug effects
;
radiation effects
;
Telomere
;
metabolism
;
Ultraviolet Rays
;
adverse effects