Using nested PCR with the specific primers for gag gene and the sequencing data from blood samples of HIV patients, we demonstrated that subtype E is also very common in the Northern area of Vietnam. This method can be applied for the cloning of HIV genes and molecular epidemiology of HIV-l in Vietnam.
HIV ; Genes ; Patients ; Classification

Rotavirus is the main cause of acute viral gastroenteritis in children under 5 years old. The study is to sequence nucleotides and amino acids of VP4, VP7, NSP1, and NSP4 genes of 5 passages of human rotavirus strain G1P8. The number of nucleotide mutations of VP4, VP7, NSP4 genes occuring among the passages were 3, 1, and 3, respectively. All these mutations resulted in changes in amino acid composition. No mutation was found in NSP1 gene.
Rotavirus ; Human ; Genes ; Child

Prospective clinical trial on two groups of patients: one treated with amitryptyline and the other with diantalvic. Results: At the beginning of study, no difference in the severity of pain in both groups. Group 1 had more effective to control pain than group 2. After one week of treatment, group 1 had one case (3.3%) while group 2 had 15 cases (35.7%) didn’t have any improvement. Amitriptyline had more advantages that Diantalvic to treat the postzosterian pain
Pain ; Amitriptyline ; Drug Combinations ; Herpes Zoster ; Therapeutics ; drugs

The authors carried out of the study on ARN message extrait from the peripheric blood of the 20 patients diagnosed to have colon cancer and 13 control healthy persons by RT - PCR quantitative with marker CK 20. The obtained results are as follows: The technique have been fulfilled; The CK 20 in the patients and the healthy person is not significant different
Colonic Neoplasms ; Blood ; RNA

A prospective study was conducted at Hung Vuong Hospital from 2002 to 2004 on total of 1423 ectopic pregnancy cases to find out determinants of operation methods, either by laparoscopy or laparotomy, in conservative treatment of unruptured ectopic pregnancy. 355 patients were enrolled in the study with 190 laparoscopic operations and 165 laparotomy operations. Factors favored to laparotomy included history of ectopic pregnancy, ectopic mass<30mm and serum o-hCG at the time of operation<1000 mUI/ml. Determinants for laparoscopic operation included maternal aged 24-35 years, blood loss<100 ml and the surgeons familiar to laparoscopy. Laparoscopy method had operation time shorter than laparotomy, and there is no difference between the two methods concerning persistent ectopic pregnancy
Pregnancy, Ectopic,Therapeutics

On 40 subjects of unspecified gender, PCR technique application for identifying DYZ1 gen to determine Y chromosome was performed in 38 subjects (95%). The results were conformed with PCR to identify TDF and the caryotype of the control group using PCR for detecting DYZ gen in determining Y chromosome. The accuracy reached 100% with no pseudonegative or pseudopositive results. The rest 5% (2 subjects) had not Y chromosome definitely. A case of TDF on X chromosome was detected affirmatively, as a first case in Vietnam.
Polymerase Chain Reaction ; genes ; Chromosomes ; analysis

The application of electron microscopy to the rapid diagnosis of viral infection is an important and integral part of diagnostic procedures. Negative staining with a solution of uranyl acetate, which is an electron-dense salt used to stain virus particles, fills the interstices of the virus surface giving the resulting electron micrograph. With ultrathin section was given that new viral encephalitis from cerebrospinal fluids of acute encephalitis syndrome patients a spherical, RNA virus, have enveloped virions about 50-60 min diameter. This virus was sensitivity with C6/36 clone of Aedes albopictus as well as many arboviruses.
Encephalitis, Viral ; Cerebrospinal Fluid ; Syndrome ; Microscopy, Electron

110 cases of unruptured ectopic pregnancy were treated, among them 61 cases (55,5%) with 1 dose of methotrexate, 40 with 2 doses (36,4) and 9 with 3 doses (8,1%). Successful rate reached 90,9% (84-95%). β- HCG took 13 (±10,3) days to return to negative point. It took 31,8 (±20,7) days for ectopic pregnant mass to disappear. Not any severe side effect was notified, except 5 cases of nausea. After treating and 12 months of follow up,47,8% of cases had got new pregnancy, among them,9,9% had reccured ectopic pregnancy. Methotrexat is an efficacious internal therapeutic for unrupted ectopic pregnancy
Methotrexate ; Therapeutics ; Pregnancy, Ectopic ; hospitals

Ovarian tumor remains a challenge for gynecologists globally. The introduction of ultrasound classification system has contributed to the evaluation of tumor nature, and then guided to an appropriate operation procedure. From 1993 to 2002, there were 226 cases of ovarian cancer operated at Hung Vuong hospital, there were included in 2 consecutive periods of without or with routineapplication of preoperative ultrasound classification system. During the second period and as a result preoperative ultrasound was done more systematically, the preoperative diagnosis was more accurate, and the disease was postoperatively staged earlier
Ovarian Neoplasms ; diagnosis ; Therapeutics

By the methods of GC and TLC, the residues of pesticides in the leaves of Crinum latifolium L. were determined after these pesticides were used for the protection of the plant. The methods of TLC and GC can be used for evaluating the residue of some pesticides.
Pesticides ; Insectiside