Disinfectants ; Fixatives ; toxicity ; Formaldehyde ; toxicity ; Paraffin Embedding ; Tissue Fixation

Aluminum ; Fixatives ; Formaldehyde ; Humans ; Polymers ; Tissue Fixation ; instrumentation ; methods

The use of resorbable implants has always been attractive to surgeons because there is no need to remove implant ai'ter fracture fixation. Other advantages include decreased load sharing, multi-taskirv ancl no metal toxicity. But the strength and stiffness of resorhable implants are less than those of metallic implants. Therefore, these implants are suitahle for fixation of particular fractwre sites such as cancellous bone and epiphyscs in which shear loads comprise the major strains. The purpose of this experimental study was to determine whether there are changes in mechanical properties and tissue reactions in the polylactic acid (PLA) rod hy surface moditication using plasma coating or hexafluoropropylene (CF3CF=CF2). PLA rods were inserted into the subcutaneous tissue of back and distal femur in rabbits. Rods in subcutaneous tissue were retrieved for material characterization and those in distal femur were ohtained for histologic observation at postoperative 2, 5, 12 and 16 weeks. The results were as follows; 1. The hydrophobicity of PLA surface was successfully ohtained by plasma coating of hexatluoropropylene gas. 2. Thcre is no significant change in tissue reaction. between controi and plasma coating PLA group. 3. The diametral strength and 3-points bending strength of plasma coating groups were higher than those of control group until postoperative 12 wks, but, diminished at postoperative 16 weeks. In conclusion, the plasma coating of PLA rod using fluorocarbon is a reasonable technique to incrcase the surface hydrophohicily and a promising method to delay the reduction of the strength of PLA rod. Further study on thicker plasma coating and Jong term effect including degradation, nsetaholism and excretion of cotated fluorocarhon will be needed.
Femur ; Fracture Fixation ; Hydrophobic and Hydrophilic Interactions* ; Plasma* ; Rabbits ; Subcutaneous Tissue

BACKGROUND: The preservation of optimized DNA and its extraction from formalin-fixed, paraffin-embedded (FFPE) tissues are important issues. There has been some doubt over whether 10% neutral-buffered formalin is an ideal fixation solution for DNA preservation over non-buffered formalin, as conventionally recommended. In this study, the correlation between the efficiency of DNA extraction from FFPE tissues and buffered formalin was evaluated. METHODS: Several tissues with same conditions except fixatives were fixed in four different formalin solution groups and were routinely processed as paraffin-embedding protocols. DNAs were extracted from four different FFPE tissues that were stored for over 3 months and over 9 months. The quantity and quality of the DNAs were assessed with a NanoDrop ND-1000 spectrophotometer, and the polymerase chain reaction (PCR) amplification and degradation were analyzed via microchip electrophoresis. KRAS mutation analysis and microsatellite instability (BAT25) PCR were performed with each sample. RESULTS: The results showed no remarkable difference in the four groups. CONCLUSIONS: The study findings demonstrate that DNA preservation is fairly unaffected by a neutral buffer where there is short formalin manufacture period and an adequate formalin fixation time before embedding in paraffin.
DNA ; Electrophoresis, Microchip ; Fixatives ; Formaldehyde ; Microsatellite Instability ; Paraffin ; Pathology, Molecular ; Polymerase Chain Reaction ; Tissue Fixation ; Tissue Preservation

Endosteum and bone marrow thermal necrosis caused by reaming during tibial intramedullary nail insertion, and unskilled operation of soft tissue penestration by reamer resulted in chronic osteomyelitis and soft tissue defect. So, several times of free flaps were done but the result was unsuccessful. At last, the authors performed radical necrotic bone resection and internal bone transport using Ilizarov external fixator. The authors report case with literature review.
Bone Marrow ; External Fixators ; Fracture Fixation, Intramedullary ; Free Tissue Flaps ; Nails ; Necrosis ; Osteomyelitis ; Tibia

PURPOSE: To evaluate the efficacy of the augmentative locking compression plate fixation in the treatment of femoral shaft nonunion occurring after intramedullary nailing. MATERIALS AND METHODS: Between July 2004 and September 2012, a total of 17 patients (twelve men, five women, average age 52.5 years) who had femoral nonunions after primary intramedullary nailing for femoral shaft fractures were reviewed. The mean period of nonunion after primary nailing was 18.5 months. Leaving the nail in situ, an augmentative locking plate was applied to the nonunion site with simultaneous autogenous bone grafting, except for five hypertrophic nonunions. We followed up all patients with plain radiograph and evaluated clinical status to determine bone union. RESULTS: All patients demonstrated evidence of fracture union with an average follow-up time of 5.0 (range 2 to 9) months. The time of operation was an average of 115 (range 45 to 160) minutes, and mean blood loss was 345.9 (range 150 to 700) ml. Two patients noted discomfort at the distal portion of plate, and one noted discomfort of donor site, but functional limitation was not observed in all patients. CONCLUSION: Augmentative locking plate fixation for diaphyseal femoral nonunion after intramedullary nailing is a reasonable treatment option with increased stability.
Bone Transplantation ; Female ; Femur ; Follow-Up Studies ; Fracture Fixation, Intramedullary* ; Humans ; Male ; Tissue Donors

Calcium carbonate(CC) is biocompatible and gradually absorb to be replaced by bone when implanted into bone tissue. Fibrin-fibronectin sealant system (FFSS) is a product of human-derived plasma. The effect is hemostasis, tissue fixation and adhesion, We expect synergic effects of this two materials in periodontal regeneration. When FFSS was grafted with bone graft in intrabony defects, could be eliminated exofolication of bone graft materials. This study evaluated above materials for periodontal regeneration of 6mm intrabony defects in 36 patients. Flap surgery was carried in 14 defects of control group. experimental group 1 was 11 defects grafted with calcium carbonate, experimental group 2 was 11 defects which were grafted with calcium carbonate with FFSS. The clinical parameters evaluated included changes in attachment level, probing depth, gingival recession at 6 months. Postsurgery probing depth reduction was 3.1 +/- 0.9mm in control, 3.8 +/- 1.6mm in experimental group 1, 4.1 +/- 1.1mm in experimental group 2. The result clinically and statistically improved compared to baseline(P<0.01), but the difference found among the groups were not statistically significant. Postsurgery clinical attachment level was 1.6 +/- 1.2mm in control, 3.5 +/- 2.0mm in experimental group 1, 3.3 +/- 1.2mm in experimental group 2. All of the control and experimental groups resulted in a statistically significant reduction from baseline(P<0.01). The reduction of the experimental groups were statistically significant from control(P<0.05). But the change between experimental group 1 and experimental group 2 was not statistically significant. We conclude that mixture of CC and FFSS is effective to periodontal regeneration in intrabony defect.
Bone and Bones ; Calcium ; Calcium Carbonate ; Carbon* ; Gingival Recession ; Hemostasis ; Humans ; Plasma ; Regeneration ; Tissue Fixation ; Transplants

Most studies related with plaque histopathology and/or morphology are based on the gray scale intravascular ultrasound (IVUS) and autopsy findings, although IVUS is limited for differentiating echolucent areas, and tissue shrinkage almost always occur during tissue fixation. In addition, autopsy studies can not establish the causal relationship between the autopsy findings and the clinical findings. Spectral analysis of the IVUS radiofrequency data may be a new and useful tool because it allows detailed assessment of plaque composition in vivo, with a high predictive accuracy of 87.1% to 96.5% in fibrous, fibrofatty, calcified and necrotic core regions with performing tissue mapping and geometric assessment like that for classic gray scale IVUS. This new imaging technique offers clear benefits compared with the results of classic IVUS and autopsy studies. This review will briefly discuss the methodology of spectral analysis of the IVUS radiofrequency data, the recent clinical studies that have used this technique and the future perspectives.
Autopsy ; Coronary Artery Disease ; Diagnostic Imaging ; Tissue Fixation ; Ultrasonography ; Ultrasonography, Interventional

Most studies related with plaque histopathology and/or morphology are based on the gray scale intravascular ultrasound (IVUS) and autopsy findings, although IVUS is limited for differentiating echolucent areas, and tissue shrinkage almost always occur during tissue fixation. In addition, autopsy studies can not establish the causal relationship between the autopsy findings and the clinical findings. Spectral analysis of the IVUS radiofrequency data may be a new and useful tool because it allows detailed assessment of plaque composition in vivo, with a high predictive accuracy of 87.1% to 96.5% in fibrous, fibrofatty, calcified and necrotic core regions with performing tissue mapping and geometric assessment like that for classic gray scale IVUS. This new imaging technique offers clear benefits compared with the results of classic IVUS and autopsy studies. This review will briefly discuss the methodology of spectral analysis of the IVUS radiofrequency data, the recent clinical studies that have used this technique and the future perspectives.
Autopsy ; Coronary Artery Disease ; Diagnostic Imaging ; Tissue Fixation ; Ultrasonography ; Ultrasonography, Interventional

PURPOSE: To evaluate the clinical and radiographic results of interlocking intramedullary nailing for the distal metaphyseal fractures of the tibia and to identify the usefulness of the interlocking intramedullary nailing. MATERIALS AND METHODS: Thirty four patients who underwent interlocking intramedullary nailing for distal metaphyseal fractures of the tibia were reviewed with a follow-up period of more than 2 years. Clinical result was assessed using the Olerud score and this score was marked as percentage of prefracture state. Radiographic results were assessed with varus-valgus angle, anterior-posterior angle, and bone union time. We checked the cases of complication and need for additional surgery after interlocking intramedullary nailing. RESULTS: Clinically, Olerud score averaged 92.1% (76~100%). Radiographically, average varus-valgus angle was 1.6+/-2.9 degrees and average antero-posterior angle was 0.8+/-3.3 degrees. Bone union time averaged 18.7 weeks. As complications, there were one deep infection and two breakages of distal interlocking screw. In additional surgery, there were 1 debridement and soft tissue flap, and one dynamization of nail at postoperative 12 weeks. CONCLUSION: Interlocking intramedullary nailing is one of safe and reliable method for distal metaphyseal fractures of the tibia, considering less soft tissue injury, possibility of early range of motion exercise, high bone union rate, and low complications rate.
Debridement ; Follow-Up Studies ; Fracture Fixation, Intramedullary* ; Humans ; Range of Motion, Articular ; Soft Tissue Injuries ; Tibia*