Adhesives ; therapeutic use ; Animals ; Humans ; Neurosurgical Procedures ; methods ; Rabbits ; Tissue Adhesives ; therapeutic use

This animal experiment was aimed to apply fibrin in facial nerve repair and to quest for technical improvements in facial surgery. In each of 15 healthy large ear white rabbits, a unilateral 5 mm intratemporal facial nerve gap was created, the proximal and distal stumps were inserted into chitin tube, 1 ml autologous fibrin glue was applied around the anastomotic zone, and no suture was employed. At 3 months and 5 months after opertion, electrophysioligical study was performed. Compared with normal nerves, the regenerating nerves in both the chitin tube bridged group and the perineurium suture group had longer incubation period, lower amplitude, slower nerve-muscle conduction velocity at 3 months postoperatively. The differences were distinctly significant (P < 0.01). Although being decreased at 5 months after operation, the differences were still statistically significant (P < 0.05). There were no significant differences between the chitin tube bridged group and perineurium suture group at 3 months and 5 months, respectively. The study suggests that facial nerve repair using fibrin glue and chitin tube has the advantages of being easier,faster and more stable.
Anastomosis, Surgical ; methods ; Animals ; Chitin ; therapeutic use ; Facial Nerve Injuries ; physiopathology ; surgery ; Female ; Fibrin Tissue Adhesive ; therapeutic use ; Male ; Nerve Regeneration ; drug effects ; Rabbits ; Tissue Adhesives ; therapeutic use

The aim of this study was to evaluate the cicatricial repair of perforating cornea in rabbits, by using the Nbutyl cyanoacrylate adhesive compared to the 910-polyglactine thread suture through macroscopic and histological assays. Corneas from 18 adult rabbits were perforated and subsequently occluded with N-butyl cyanoacrylate synthetic adhesive (right cornea) or by separated single points using the 910-polyglactine thread (left cornea). The rabbits were divided into groups containing three animals per group. Examination after 7, 15, and 30 days post-operative showed that both the synthetic adhesive and the suture were efficient in the occlusion of the surgical wounds, thus stabilizing the intra-ocular content. The N-butyl cyanoacrylate adhesive was shown to be superior to the 910-polyglactine suture thread with regards to the evolution and the organization of the healing process.
Animals ; Cornea/*injuries/*surgery ; Enbucrilate/*therapeutic use ; Female ; Male ; Polyglactin 910/therapeutic use ; Rabbits/*surgery ; *Sutures ; Tissue Adhesives/*therapeutic use ; *Wound Healing

OBJECTIVETo investigate the efficacy of tissue adhesive on intractable bleeding during endoscopic submucosal dissection(ESD) and delayed bleeding.

METHODSA total of 9874 patients with gastrointestinal mucosal or submucosal tumors underwent ESD in our center from September 2006 to August 2013 and intractable bleeding occurred during ESD in 5 cases. Under the condition of no effective hemostasis methods, the tissue adhesive injection or spray were used to stop the bleeding. The efficacy and safety were evaluated.

RESULTSAll the 5 cases were successfully managed by the hemostasis method with tissue adhesive without any adverse event. In follow-up of two months after operation, wound healing and scar formation were observed under endoscopy.

CONCLUSIONTissue adhesive is safe, effective and fast for intractable bleeding during ESD and delayed bleeding.

Dissection ; Endoscopy, Gastrointestinal ; Gastric Mucosa ; surgery ; Hemorrhage ; etiology ; therapy ; Humans ; Intestinal Mucosa ; surgery ; Tissue Adhesives ; therapeutic use ; Treatment Outcome

Adult ; Colloids ; therapeutic use ; Epistaxis ; etiology ; therapy ; Female ; Humans ; Liver Transplantation ; adverse effects ; Male ; Middle Aged ; Perioperative Period ; Tissue Adhesives ; therapeutic use ; Treatment Outcome

OBJECTIVETo investigate the feasibility and effectivity of using compound material of fibrin glue and DBM as scaffolds for cartilage tissue engineering.

METHODSChondrocytes isolated from articular cartilage were seeded into prepared scaffolds, after incubation for 4 weeks in vitro. Chondrocytes and fibrin glue and DBM constructs were implanted in the joint cave of rabbit. The specimens were excised at the 4th, 8th, 12th week, examined grossly analyzed by haematoxylin cosine, toluidine blues staining and type II collagen immunohistochemistry reaction. Wakitani score was counted to evaluate the repairing effect.

RESULTSGrossly analysis showed some ivory tissue filled the caves after 4 weeks and the caves were full filled with smooth surface after 12 weeks. The microscope showed a good deal of chondrocytes appeared after 8 weeks and more type II collagen than 4 weeks. Twelve weeks later, cartilage lacuna could be observed. The cells arrangement and the amount of type II collagen both showed the same as the natural one.

CONCLUSIONComplicated material of fibrin glue and DBM as scaffolds can be used as scaffolds for cartilage tissue engineering.

Animals ; Bone Matrix ; physiology ; transplantation ; Cartilage, Articular ; physiopathology ; surgery ; Cell Transplantation ; Cells, Cultured ; Chondrocytes ; physiology ; transplantation ; Female ; Fibrin Tissue Adhesive ; therapeutic use ; Male ; Rabbits ; Random Allocation ; Regeneration ; Tissue Adhesives ; therapeutic use ; Tissue Engineering ; Tissue Scaffolds

During clinical operation, the repeated bonding of octyl-a-cyanoacrylate adhesive is necessary for satisfactory reduction and fixation. This is because the repeated bonding will change the morphologic characteristics of the bone surface in biomechanical study. The influence of repeated bonding of octyl-a-cyanoacrylate on fresh human tibia cortical bone was assessed in this experiment. First, we made the transverse fracture model on the anterior part of cortical bone of fresh human tibia shaft. After 24 hours of bonding, the tensile strength, shear strength and angular strength were tested; then we made the bonding for the second time and third time, tested the tensile strength, shear strength and angular strength respectively, and observed the change of tensile strength, shear strength and angular strength. We found the shear strength of the primary bonding being greater than that of the second bonding and the third bonding (P<0.05). The shear strength of the second bonding and the third bonding showed no statistically significant difference. The tensile strength, elastic modulus, rigidity coefficient and moment of area inertia exhibited no statistically difference between the first, second and third bonding. The repeated bonding of octyl-a-cyanoacrylate adhesive will change the morphologic characteristics of the bone surface, this will decrease the shear strength during the experiment. When the shear strength test is to be repeated, the residue of the adhesive on the surface of the bone bonded area should be removed. During clinical application, bonding should be performed only once in the area for resisting the shear strength.
Biomechanical Phenomena ; Bone Cements ; chemistry ; therapeutic use ; Cyanoacrylates ; chemistry ; therapeutic use ; Elastic Modulus ; Fracture Healing ; Humans ; Shear Strength ; Tensile Strength ; Tibia ; Tibial Fractures ; therapy ; Tissue Adhesives ; chemistry ; therapeutic use

A medical hard tissue adhesive, octyl-a-cyanoacrylate, was tested in 6 fresh human tibiae. A 90 degrees butter-fly fracture fragment was made in the middle part of tibia by bandsaw. The compressive stress, torsional stress and angular deflection were assessed before and after osteoectomy respectively. After adhesive bonding, the compressive stress, torsional stress and angular deflection were tested again. The butterfly fracture fagment decreased the bending strength, torsion strength, yielding strength of tibia bone. In torsion test, the torque of tibia before osteoectomy is greater than bonded tibia, the bonded tibia is greater than that of the unbonded tibia. In compression test, before adhesive bonding broken, the compressive curve slope of tibia before osteoectomy is greater than that of bonded tibia, the bonded tibia is greater than that of the unbonded tibia. In angular deflection test before adhesive bonding of broken,the curve slope of tibia before osteoectomy is not different from that of bonded tibia (P>0.05), the slope the bonded tibia is greater than the slope of unbonded tibia(P<0.05). The elastic modulus, rigidity coefficient and moment of area inertia show no statistical difference between the bonded tibia and intact tibia. The used of medical hard tissue adhesive to bond the fracture fragment could improve the bending strength, torsion strength, yielding strength of tibia bone. In operation, it can reduce the soft tissue injury when the fracture fragment is being fixed, and this will benefit bone healing.
Biomechanical Phenomena ; Bone Cements ; therapeutic use ; Cyanoacrylates ; chemistry ; therapeutic use ; Fracture Fixation, Internal ; methods ; Fracture Healing ; Fractures, Comminuted ; surgery ; Humans ; Materials Testing ; Tensile Strength ; Tibia ; surgery ; Tibial Fractures ; surgery ; Tissue Adhesives ; chemistry ; therapeutic use

This randomized prospective study investigated the effect of fibrin glue use on drainage duration and overall drain output after lumpectomy and axillary dissection in breast cancer patients. A total of 100 patients undergoing breast lumpectomy and axillary dissection were randomized to a fibrin glue group (N=50; glue sprayed onto the axillary dissection site) or a control group (N=50). Outcome measures were drainage duration, overall drain output, and incidence of seroma. Overall, the fibrin glue and control groups were similar in terms of drainage duration, overall drain output, and incidence of seroma. However, subgroup analysis showed that fibrin glue use resulted in a shorter drainage duration (3.5 vs. 4.7 days; p=0.0006) and overall drain output (196 vs. 278 mL; p=0.0255) in patients undergoing level II or III axillary dissection. Fibrin glue use reduced drainage duration and overall drain output in breast cancer patients undergoing a lumpectomy and level II or III axillary dissection.
Adult ; Axilla ; Breast Neoplasms/pathology/*surgery ; Drainage ; Female ; Fibrin Tissue Adhesive/*therapeutic use ; Humans ; *Lymph Node Excision ; *Mastectomy, Segmental ; Middle Aged ; Prospective Studies ; Seroma/epidemiology/etiology ; Severity of Illness Index ; Time Factors ; Tissue Adhesives/*therapeutic use

OBJECTIVE: To evaluate the fixation strength and tissue reaction of the glue fixation and self-stabilizing leg fixation methods and to compare the results with those of the conventional tagging suture fixation method. MATER AND METHODS: Twelve healthy rabbits were selected and three different methods of implanting the port chamber were employed on the back of each rabbit. A total of thirty six port chambers were implanted with these three different methods, viz. the glue fixation method using tissue adhesive, the self-stabilizing leg method using a self-expandable stabilizing leg, and the suture fixation method. The fixation strength and the gross and histopathologic changes of each fixation method were evaluated at three days, one week, two weeks and four weeks after port implantation. RESULTS: The glue fixation method showed a good fixation strength, which was similar to that of the tagging suture method (p=0.3486). Five of the six ports (83%) implanted with the glue fixation method which were examined after two weeks showed cracks on the external surface, but this had no adverse effects on their function. A large amount of granulation tissue reaction was found at the bottom of the chamber (p=0.0025). The fixation with the self-stabilizing leg showed relatively lower fixation strength (p=0.0043), but no turning-over of the chamber occurred. The fixation strength improved with time after the first week, and minimal granulation tissue reaction was observed with this method. CONCLUSION: The glue fixation method exhibited equal fixation strength compared to the suture fixation, but showed cracking and a large amount of granulation tissue, whereas the fixation with a self-stabilizing leg showed weaker fixation strength.
Alloys ; Animals ; Capillaries/cytology/metabolism/pathology ; Cell Proliferation ; Device Removal ; Enbucrilate/therapeutic use ; *External Fixators ; Fibroblasts/metabolism/pathology ; Granulation Tissue/blood supply/metabolism/pathology ; *Implants, Experimental ; Models, Animal ; Rabbits ; Sutures/*utilization ; Time Factors ; Tissue Adhesives/*therapeutic use