The purpose of this study was to compare corneal wound healing between suture and sutureless lamellar keratoplasty in the rabbit eye. The tissue adhesive (Tisseel, Vienna) a commercially available two component adhesive system based on human fibrinogen, which is activated by thrombin, was used. 8.0mm half-thickness lamellar keratoplasties (autotransplants) were performed on the twenty-four rabbit eyes with tissue adhesive and the other eyes were operated with 10-0 nylon continuous suture as a control, respectively. At varying periods postoperatively, they were killed and the eyes were immediately enucleated and examined by transmission electron microscopy and light microscopy. The procedure was completed by the application of a bandage soft contact lens. Seventy-one percent of the glued lamellar keratoplasty grafts were retained and in six eyes, focal white opacities at the bed and graft interface were noted. There was no change in corneal contour, with the only irregularity being at the graft/host junction in the tissue adhesive group. Histopathologic examination of the glued eyes demonstrated the presence of a layer of eosinophilic material between the corneal bed and the lamellar graft but a minimal inflammatory response was observed in the early postoperative days. At fourteen days the adhesive was no longer visible and at three weeks, the graft was well healed. We believe that this study indicates a potential adjunctive role for tissue adhesive in corneal wound healing.
Animals ; *Corneal Transplantation/methods ; Fibrin Tissue Adhesive/*pharmacology ; Postoperative Complications/pathology ; Rabbits ; Tissue Adhesives/*pharmacology ; Wound Healing/*drug effects

PURPOSE: To evaluate the clinical efficacy and histopathological tolerance of 2-octyl cyanoacrylate versus conventional suture materials for eyelid wound closure in rabbits. METHODS: We performed an experimental study on 16 eyes of eight New Zealand albino rabbits. Eyelid incisions of 15 mm were done 4mm from the upper eyelid margin in both eyes. The eyes of the rabbits were divided into two groups: eyelid incisions of the right eye were closed by a 2-octyl cyanoacrylate adhesive (group A) and eyelid incisions of the left eye were closed by 7-0 nylon sutures (group B). At 1, 2, 4, and 8 weeks after surgery, the rabbits were macroscopically examined and then sacrificed. The specimens of their eyelid tissues were stained by a hematoxylin and eosin stain and Masson-trichrome stain, and were observed under microscope. RESULTS: Both eyelid surgical closure methods were found to be equally efficacious in fixing the eyelids of groups A and B, and their clinical efficacy was similar. Histopathological findings of the hematoxylin and eosin stain of group A showed less inflammatory infiltration than group B at 2 weeks. There were no significant histopathological differences between the two groups at 1, 4, and 8 weeks. The degree of fibrosis of the Masson-trichrome stain was similar between the two groups at 8 weeks. CONCLUSIONS: The 2-octyl cyanoacrylate adhesive proved to be an effective eyelid closure method and was very well tolerated by the skin surface. 2-Octyl cyanoacrylate could be used as an alternative tissue adhesive for eyelid wound closure along with conventional suture materials.
Animals ; Blepharoplasty/*methods ; Cyanoacrylates/*pharmacology ; Disease Models, Animal ; Eyelid Diseases/pathology/*surgery ; Eyelids/*surgery ; Rabbits ; Suture Techniques/*instrumentation ; *Sutures ; Tissue Adhesives/*pharmacology ; Treatment Outcome ; Wound Healing

Mussel adhesive proteins have attracted increasing interests for their potential use as environmentally friendly bioadhesives in medicine and aqueous conditions. In this study, surface coating analysis, quartz crystal microbalance (QCM), cell and bone tissue adhesion and cytotoxicity assay were used to study the properties of the Perna viridis foot proteins (Pvfp) extract as bioadhesive. The results of coating ability on various materials and QCM analysis revealed that Pvfp extract has comparable or superior adsorbtion ability to that of Cell-Tak (the naturally extracted MAP mixture from Mytilus edulis, and has been commercialized), and also, the cell adhesion ability of Pvfp extract was stronger than that of Cell-Tak and poly-L-lysine. No cytotoxicity was detected using human HeLa and 293T cells. Furthermore, broken bones of mouse could be stuck together by use of Pvfp extract. In bulk-scale adhesion tests, Pvfp extract showed much greater tensile strength than did fibrin glue for conglutinating poly (vinl chloride) sticks and for binding together pig's femur segments. These results suggested that Pvfp extract be an efficient cell and tissue adhesive in biotechnological application and it might be a potential bioadhesive in medical practice.
Animals ; Bone Cements ; isolation & purification ; Bone and Bones ; drug effects ; HeLa Cells ; Humans ; Mice ; Perna ; chemistry ; Proteins ; isolation & purification ; pharmacology ; Swine ; Tissue Adhesives ; isolation & purification

PURPOSE: To compare the effect of using fibrin glue or 10-0 nylon sutures on the clinical outcome of patients undergoing pterygium excision and conjunctival autografting. METHODS: We retrospectively reviewed the medical records of 52 eyes from 46 patients who underwent pterygium excision and conjunctival autografting and were followed up for more than 3 months. The operation duration, postoperative inflammation, complications, and recurrence rates were compared between groups of 20 patients (22 eyes) for whom fibrin glue was used (fibrin glue group) and 26 patients (30 eyes) for whom suturing was performed with 10-0 nylon (suture group) in pterygium excision and conjunctival autografting. RESULTS: The operation duration was 27.71 (5.22) minutes in the fibrin glue group and 43.30 (8.18) minutes in the suture group (p = 0.000). Seven days after the operation, the fibrin glue group showed milder conjunctival inflammation than the suture group (p = 0.000). Postoperative complications and corneal recurrence rates were not statistically different between the two groups. CONCLUSIONS: The use of fibrin glue in pterygium excision with conjunctival autografting is likely to be a more effective, safer procedure than suturing.
Adult ; Aged ; Aged, 80 and over ; Conjunctiva/*transplantation ; Female ; Fibrin Tissue Adhesive/*pharmacology ; Follow-Up Studies ; Humans ; Male ; Middle Aged ; Patient Satisfaction ; Pterygium/*surgery ; Retrospective Studies ; Suture Techniques/*instrumentation ; *Sutures ; Time Factors ; Tissue Adhesives/pharmacology ; Transplantation, Autologous