1.First Record of Paramphistomes Fischoederius cobboldi and Paramphistomum epiclitum Detected in Bovine Rumen from a Local Market of Savannakhet Province, Lao PDR.
Surapol SANGUANKIAT ; Marcello Otake SATO ; Megumi SATO ; Wanna MAIPANICH ; Tippayarat YOONUAN ; Tiengkham PONGVONGSA ; Boungnong BOUPHA ; Yuichi CHIGUSA ; Kazuhiko MOJI ; Jitra WAIKAGUL
The Korean Journal of Parasitology 2016;54(4):543-547
In the present study, we report on the occurrence of paramphistomes, Fischoederius cobboldi and Paramphistomum epiclitum, in Lao PDR with the basis of molecular data. Parasite materials were collected from bovines bred in Ban Lahanam area, Savannakhet Province, Lao PDR at Lahanam public market. Morphological observations indicated 2 different species of paramphistomes. The mitochondrial gene cox1 of the specimens was successfully amplified by PCR and DNA sequencing was carried out for diagnosis of 11 specimens. Pairwise alignment of cox1 sequences were performed and confirmed F. cobboldi and P. epiclitum infecting bovines in Laos. Although there were many limiting points, as the small number of worm samples, and the restricted access of the animal host materials, we confirmed for the first time that 2 species of paramphistomes, F. cobboldi and P. epiclitum, are distributed in Lao PDR. More studies are needed to confirm the paramphistome species present in Savannakhet and its hosts to clear the natural history of these parasites of ruminants in the region and measure the impact of this parasite infection in the life and health of the local people.
Animals
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Cattle
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Diagnosis
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Genes, Mitochondrial
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Helminths
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Laos
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Natural History
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Paramphistomatidae*
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Parasites
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Polymerase Chain Reaction
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Rumen*
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Ruminants
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Sequence Analysis, DNA
2.Genetic Diversity of Taenia asiatica from Thailand and Other Geographical Locations as Revealed by Cytochrome c Oxidase Subunit 1 Sequences.
Malinee Thairungroj ANANTAPHRUTI ; Urusa THAENKHAM ; Dorn WATTHANAKULPANICH ; Orawan PHUPHISUT ; Wanna MAIPANICH ; Tippayarat YOONUAN ; Supaporn NUAMTANONG ; Somjit PUBAMPEN ; Surapol SANGUANKIAT
The Korean Journal of Parasitology 2013;51(1):55-59
Twelve 924 bp cytochrome c oxidase subunit 1 (cox1) mitochondrial DNA sequences from Taenia asiatica isolates from Thailand were aligned and compared with multiple sequence isolates from Thailand and 6 other countries from the GenBank database. The genetic divergence of T. asiatica was also compared with Taenia saginata database sequences from 6 different countries in Asia, including Thailand, and 3 countries from other continents. The results showed that there were minor genetic variations within T. asiatica species, while high intraspecies variation was found in T. saginata. There were only 2 haplotypes and 1 polymorphic site found in T. asiatica, but 8 haplotypes and 9 polymorphic sites in T. saginata. Haplotype diversity was very low, 0.067, in T. asiatica and high, 0.700, in T. saginata. The very low genetic diversity suggested that T. asiatica may be at a risk due to the loss of potential adaptive alleles, resulting in reduced viability and decreased responses to environmental changes, which may endanger the species.
Animals
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Asia/epidemiology
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Cluster Analysis
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Electron Transport Complex IV/*genetics
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*Genetic Variation
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Genotype
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Humans
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Molecular Sequence Data
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*Phylogeography
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Protein Subunits/genetics
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Sequence Analysis, DNA
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Taenia/*classification/*genetics/isolation & purification
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Taeniasis/*epidemiology/*parasitology
3.Asymptomatic Human Paragonimiasis among the Karen People in Tak Province, Thailand: A Case Report
Teera KUSOLSUK ; Orawan PHUPHISUT ; Wanna MAIPANICH ; Somchit PUBAMPEN ; Surapol SA-NGUANKIAT ; Akkarin POODEEPIYASAWAT ; Nirundorn HOMSUWAN ; Srisuchart MONGKOLMOO ; Tippayarat YOONUAN ; Poom ADISAKWATTANA ; Udomsak SILACHAMROON ; Yukifumi NAWA
The Korean Journal of Parasitology 2020;58(1):57-60
During the mobile clinic activities in Tak Province, Thailand, Paragonimus sp. eggs were found in a fecal sample of a 72-year-old Karen resident. Paragonimus DNA was amplified from the stool sample and identified to P. heterotremus. The patient did not have any symptoms. Apparent pulmonary lesion was not found on the chest X-ray. The patient admitted habitual consumption of semi-cooked or roasted waterfall crabs for several years. The waterfall crabs collected from stream near the village were found negative for Paragonimus metacercariae. In northern Thailand, paragonimiasis remains as one of the public health concerns and should be ruled out for asymptomatic pulmonary patients.