139 subjects of 2 ethnic groups of Kinh (Hanoi) and Muong (HoaBinh) were submitted to study from October to December 2003 in order to assess the sensitivity and the specificity of the rapid test of determination of G6PD. All subjects underwent both two methods (rapid test and quantifying method) in concomittance.Results showed the sensity of 91,2% and the specificity of 97,2% in both male and female genders. For detecting G6PD defiency in male, the rapid test gave higher sensitivity and specificity than in female 97,1 and 100% respectively in male and 85,3% and 94,1% in female subjects
Glucosephosphate Dehydrogenase ; deficiency ; diagnosis ;

To determine the prevalence of G6PD deficiency, the cross sectional surveys were carried out in 5922 subjects (4,043 males and 1,879 females) from 14 different ethnic groups of population in 11 provinces of Vietnam from 1996 to September 2004. Two methods with qualitative virual fluorescent test and rapid test were used. The samples from 559 individuals were independently and comparatively analyzed by two methods for calculating the Kappa index. The Kappa index of 0.081 reflected a high compatibeness of two methods. The prevalence of G6PD deficiency varied from area to area, and D6PD of males was much different from group to group population. There was no relationship between G6PD and malaria
Glucosephosphate Dehydrogenase ; epidemiology ; deficiency

A cross-sectional surveys on 1.206 children aged < 13 years old were carried out in Tra Mai commune of Quang Nam province and Phu Rieng commune of Binh Phuoc province in 2 years of 1999 and 2000. The proportion of anaemia children in Tra Mai was 21.6%, Phu Rieng was 33.5%. The most of them were mild anaemia patients. No severe anaemia case was detected. Malaria, hookworm were determined to be the risk factors for anaemia. There was a correlation between the hemoglobin concentration, the malaria parasitemia and the intensity of hookworm infection. Acid folic supplement treatment with dose of 2mg/kg/day x 30 days, combined with specific drugs in anaemia children had increased clearly hemoglobin concentration compared with single specific drugs
Malaria ; Parasitemia ; child ; epidemiology ; Helminthiasis ; Parasitic Diseases ; Anemia

Background: Paracheck F.test is rapid diagnostic test that use to determine presence of histidine rich protein 2-HPR-2, a component of Plasmodium falciparum in total blood. Paracheck F.test producted by India\r\n', u'Objective: To study the sensitivity, specificity and diagnosed conformity of Rapid Diagnosis Test (Paracheck F test) compared with microscopic method for detecting P.falciparum \r\n', u'Subject and method: The study was conducted in 5 communes of Quang Tri province and 1 commune of Quang Binh province from September to November in 2006. A total of 470 blood samples were collected and examined with 2 methods for detecting P falciparum (Microscopy and Rapid Diagnosis Test- Paracheck). \r\n', u'Results:Among these blood samples, there were 438 samples having complete conformity in both two methods. 29 samples were negative with microscopy but positive with Paracheck. 3 samples were positive with Paracheck but negative with microscopy.\r\n', u'Conclusion: the sensitivity and specificity of Paracheck is 99% and 82%, respectively. Both microscopic and Paracheck methods have a high conformity in detecting P falciparum with the Kappa index of 0.84. \r\n', u'
Sensitivity ; specificity ; diagnosed conformity ; Paracheck F test ; microscopy

Background: WHO recommends that malaria drug should be used with essential elements which are derivatives of artemisinin (ART) for treatment phase and limit the development of parasite (MIC). Objective: To assess in vitro effect of artemisinin powder and 10 alpha- trifluoro methyl hydroartemisinin (TEMHA) in powder and tablet form to P.falciparum. Subject and Method: 48h in vitro test of Phuc Nguyen Dinh was applied to this study. Results and Conclusions: The results showed that: for T996, IC50 values of ART, 10 alpha- TEMHA powder and 10 alpha- TEMHA pill were as follows: 37.8; 16.4 and 17.6 nM/L, respectively. For K1, IC50 values of ART, 10 alpha- TEMHA powder and 10 alpha- TEMHA pill were: 22.8; 11.4 and 12.2 nM/L, respectively. MIC values of artemisinin powder, 10 alpha- TEMHA powder and pill for T996 were as follows: 100; 40 and 40nM/L, respectively. For K1, MIC values of ART, 10 alpha- TEMHA powder and pill are: 76; 24; 32 nM/L, respectively.
10 a- trifluoro methyl hydroartemisinin ; P.falciparum

Background: In 2006, the project of global fund for malaria prevention in Vietnam provided a large number of rapid diagnostic test Paracheck F for Vietnam for the purpose of rapid malaria diagnose. However, there is no study on evaluation the effect of rapid diagnostic test compared with microscopy method. Objective: To evaluate the diagnostic yield and cost of paracheck F test and microscopy in malaria diagnosis and treatment. Subject and Method: The study was carried out in 6 communes belongs to Quang Tri and Quang Binh provinces from September to November - 2006. The study was divided into 3 phases. Phase 1: diagnoses and treatments are based on clinical symptoms, phase 2: diagnoses and treatments are based on the results of paracheck and phase 3: diagnoses and treatments are based on the results of microscopy. All phases, both the common patients and malarial patients and the amount of anti-malarial drugs were treated, the amount of money was spent on transport and days work off of malarial patients and their relatives were calculated. Result: The investigation data on expenditure of malaria patients showed that: the average direct cost of malaria patient in phase 1 is VND 116.100; phase 2: VND 119.400 and phase 3: VND 120.800 per 1 treatment course. There is no significant difference between direct costs in three phases (p > 0.05). Conclusion: The expense efficiency for finding out a case of malaria by paracheck and microscopy is equivalent and lower than the expense of diagnosis based on clinical symptoms.
Malaria diagnosis ; RDT Paracheck F ; microscopy