Objective To investigate protective effects of polysaccharides from Platycarya strobilacea Sieb.et Zucc on cerebral ischemia-reperfusion injury in rats.MethodsForty Wistar rats were randomly divided into four groups:sham group,model group,Rehmannia glutinosa polysaecharide low,and high dose groups.Each group was given oral administration for 7 days.After 1 h of last administration, the CIRI (cerebral ischemia-reperfusion injury) group was produced inserting 5-0 line into the internal carotid artery.Neurological functional score was evaluated according to the method of Zea longa's score, meanwhile,infarct size was detected by TTC staining.Levels of superoxide dismutase (SOD) activity,malondialdehyde (MDA), IL-10, IL-1β and TNF-α contents in cerebral tissues were measured.TUNEL staining was used to assess the number of TUNEL-positive cells of the ischemic cortex.Western blot was used to analyze the expressions of Nrf2 and HO-1 in cerebral tissues.ResultsCompared to model group, Platycarya strobilacea a polysaccharides could significantly improve neurogical functions,greatly decrease the contents of MDA, IL-10 and IL-1β in cerebral tissues,improve SOD activity and IL-10 content in cerebral tissues,and effectively reduce cerebral infarct range.Meanwhile Platycarya strobilacea polysaccharides could enhance the expressions of Nrf2 and HO-1 in cerebral tissues.ConclusionPlatycarya strobilacea polysaccharides have beneficial effects on rats with cerebral ischemia-reperfusion injury, which may be mediated at least in part by the Nrf2/HO-1 pathway.

Metallothionein is a sulfhydryl-rich,and chelating a large number of metal ions,which is widely found in biological world.It is very highlighted that MT had the same physical and chemical features from different origins.Plenty of research data indicated that MTs contain 20 free sulfhydryl genes,as well as their sulfhydryl clusters,It is a multifunctional protein in health.Many experiments demonstrate that MTs may be a novel target of original new drugs because they play a role in regulating and controlling the organ fibrosis and tumor.

Objective To investigate the protective effects of luteolin on cerebral ischemia-reperfusion injury in rats.Methods 40 SD rats were randomly divided into four groups:sham group,model group,luteolin low and high dose groups.Each group was given oral administration for 7 days.After 1 h of last administration,the CIRI ( cerebral ischemia-reperfusion injury) group was produced inserting 5-0 line into the internal carotid artery.Then levels of IL-10, IL-1βand TNF-αcontents in myocardial tissues were measured.Meanwhile,infarct size was detected by TTC staining and number of BrdU +and Nestin + were measured by immunohistochemical methods.Results Compared with model group, luteolin could significantly decrease the levels of TNF-αand IL-1βin cerebral tissues(P<0.05),improve IL-10 content in cerebral tissues(P<0.05),and effectively reduce myocardial infarct range(P<0.05).Meanwhile luteolin could improve the expressions of BrdU + and Nestin + in cerebral tissues(P <0.05).Conclusion Luteolin has beneficial effects on rats with cerebral ischemia-reperfusion injury and improves endogenous neural stem cells proliferation.

Objective To improve the knowledge of paediatric pulmonary arterial hypertension(PAH) and to elevate the level of early diagnosis and treatment.Methods The clinical data of 45 PAH patients admitted in Shanghai Children's Hospital from Jan.2006 to Dec.2012 were reviewed,including clinical manifestation,laboratory examination,diagnosis,treatment and prognosis.Results Of the 45 PAH patients,21 cases (46.7％) were male and 24 cases (53.3 ％) were female,with an average age of 2.5 years old.Among them idiopathic PAH was in 24 cases (53.3 ％) and secondary PAH was in 21 cases [including interstitial lung disease in 4 cases,upper airway obstruction in 3 cases,systemic lupus erythematosus in 3 cases,hepatic disease in 3 cases,including hepatic hemangioma 1 case,liver cirrhosis portal hypertension 1 case and autoimmune hepatic sarcoidosis 1 case,tachycardia induced cardiomyopathy in 2 cases,extensive pulmonary small artery stenosis in 2 cases,human immunodeficiency virus (HIV) infection in 1 case,hypothyroidism in 1 case,and familial PAH in 2 cases].Main clinical manifestations were anhelation after exercise (71.1％),fatigue (68.9％),cough (48.9％),chest tightness (26.7％),chest pain (33.3％),syncope (8.9％),et al.The most common physical signs were splitting of the second sound in pulmonary valve area (93.3％),followed by tricuspid murmur (77.8％),cyanosis (53.3％),hepatomegaly (42.2％),hydropericardium (28.9％),and oedema (11.1％),et al.Twenty-seven cases did cardiac catheterization,compared with idiopathic PAH and secondary PAH,pulmonary artery systolic pressure,mean pulmonary artery pressure,pulmonary capillary wedge pressure,pulmonary arteriolar resistance index had significant difference(P ＜ 0.05).Thirty-one cases' condition improved after treatment,11 cases without any improvement and 3 cases died during follow-up visit.Conclusions PAH is a rare disease with no specific symptom and can be easily misdiagnosed in children.Ultrasonic cardiogram and cardiac catheterization are helpful in diagnosis.Actively looking for the cause can improve the prognosis.

Objective To explore the effect of different diary method on daily affect of college students.Methods 75 numbers of under-graduates were randomly assigned into information recording group,feeling recording group and control group.Beck Depression Inventory (BDI) and Positive And Negative Affect Scale (PANAS) were used before and after two months intervention.Results For positive emotion,ANOVA results showed no significant (P＞0.05) main effect of time,a significant (P＜0.05) main effect of group(2.76±0.41,2.97±0.50,3.24±0.45),and the time × group had a significant interaction (P＜0.05).Simple effect analysis showed that after the intervention,the positive emotions of information recording group (2.47 ± 0.62) and feeling recording group (2.86±0.64) was lower than the positive emotions of control group(3.35±0.46) significantly(P＜0.05).For negative emotions,there was no significant difference (P＞0.05) in main effects of time,group and interaction;for depression,the main effect of the level of time (8.02± 5.65,5.38 ± 5.04,5.20± 5.51) was significant (P＜ 0.01),and main effect of group and the interaction were not significant(P＞0.05).Conclusion Different diary methods have different effects on the subject's daily affect.

Objective To investigate the relationship between interleukin 1β( IL?1β) , interleukin?33 ( IL?33) , neutrophil?to?lymphocyte ratio ( NLR ) and atrial fibrillation. Methods Eighty?two patients with nonvalvular atrial fibrillation treated in the department of cardiology in the Fourth People′s Hospital of Jinan from October 2015 to October 2016 were enrolled in the study,including 43 patients with persistent atrial fibrillation and 39 patients with paroxysmal atrial fibrillation. 50 healthy subjects were selected as the control group. Enzyme linked immunosorbent assay (ELISA) was used to determine the concentration of IL?1βand IL?33,and the left atrial diameter ( LAD) was measured by echocardiography. Results ( 1) The concentrations of IL?1β,NLR and LAD in the paroxysmal atrial fibrillation group were (24. 44±4. 89) ng/L,(2. 51±1. 22) %,(36. 16± 6. 12) mm,the concentrations of IL?1β,NLR and LAD in the persistent atrial fibrillation group were (26. 95±5. 86) ng/L,(5. 7±1. 8) %,(39. 36±4. 78) mm and the values in the control group were (19. 53±4. 51) ng/L,(1. 82 ± 0. 41 ) %, ( 33. 31 ± 2. 89 ) mm, respectively. The differences among the three groups were statistically significant ( F=16. 74,11. 82,14. 85,P<0. 01) . The indexes of paroxysmal atrial fibrillation group and persistent atrial fibrillation group were higher than those of the control group ( P<0. 01 ) . ( 2 ) In the persistent atrial fibrillation group, NLR and LAD were ( 5. 7 ± 1. 8 )% , and ( 39. 36 ± 4. 78 ) mm, higher than those of the paroxysmal atrial fibrillation group ( (2. 51±1. 22)%,(36. 16±6. 12) mm),the differences were statistically significant ( P<0. 01) . However,the level of IL?1βin the persistent atrial fibrillation group was not significantly different from that in the paroxysmal atrial fibrillation group ( (26. 95±5. 86) ng/L vs. (24. 44±4. 89) ng/L,P>0. 05). (3) The concentrations of IL?33 in the paroxysmal atrial fibrillation group,atrial fibrillation group, control group were ( 48. 31 ± 4. 72 ) ng/L, ( 50. 03 ± 2. 18 ) ng/L, ( 56. 87 ± 5. 12 ) ng/L, respectively. The difference among the three groups has no statistical significance ( F=2. 52, P>0. 05 ) . ( 4 ) NLR level was positively correlated with LAD ( r=0. 32,P=0. 002) . There was no significant correlation among IL?1β,IL?33 and LAD ( r=0. 16, P=0. 11, r=0. 02, P=0. 37 ) . Conclusion The levels of IL?1β, NLR and LAD in peripheral blood of patients with atrial fibrillation were significantly higher than those in patients with sinus rhythm,and there was a positive correlation between NLR and LAD.

Objective To explore the clinical characteristics and treatment of paroxysmal supraventricular tachycardia (PSVT) in children. Methods The clinical data of 67 children with PSVT were analyzed retrospectively, and the therapeutic effects of different treatments were compared. Results The clinical manifestations of infants were paleness, shortness of breath, irritability and sweating, and children showed chest tightness, palpitations, abdominal discomfort and fatigue. The curative effect of electric cardioversion, transesophageal atrial pacing, physical therapy, and drug therapy was statistically different (P<0.05), The different cardioversion rates of them were observed for the treatment of paroxysmal supraventricular tachycardia. The cardioversion rate of transesophageal atrial pacing, was the highest, and the rate of physical therapy was the lowest. There was no significant difference in the cardioversion rate between propafenone, digoxin and amiodarone. Conclusion The clinical manifestations of PSVT in infants are atypical and easily to be ignored. There are many methods for treatment of PSVT. The vagus nerve can be stimulated first, and, if no response, either drugs or electric cardioversion and transesophageal atrial pacing can be used. The cardioversion rate of transesophageal atrial pacing is higher. The drug effectiveness for the treatment of PSVT depends on many factors, and our choice of medication varies from person to person.

Objective To study the effect of telbivudine (TBV) on the ratio of CD4+ CD25+CD127low/-regulatory T cell (Treg) of HBeAg-positive chronic hepatitis B (CHB). Methods The ratios of Treg before and after treatment for 12, 24, 36 and 48 weeks with TBV of thirty-five patients with HBeAg-positive CHB were detected. Results Ratios of Treg were (12.55 ± 1.78)%, (10.42 ± 1.04)%, (9.55 ± 0.74)%, (8.78 ± 0.67)%, (8.74 ± 0.71)%respectively before and after treatment of 12, 24, 36 and 48 weeks and all the values were lower than its former one (P < 0.05), except the 48 weeks′. Conclusions The ratio of Treg of HBeAg (+) CHB decreases after treatment with TBV.

Objectives To explore the clinical features and pathogenesis of Kawasaki disease (KD) combined with sterile pyuria. Methods A total of 420 patients diagnosed of KD were recruited and divided into pyuria group ( 95 patients) and control group ( 325 patients) according to urine routine examination on admission. The clinical data between the two groups were compared. Results There was no difference in gender, age, and the incidence of atypical KD (P all?>?0 . 05 ). The levels of C-reactive protein, D-dimer concentrations, fibrinogen degradation products, alanine aminotransferase, aspartate aminotransferase, and urine retinol binding protein were higher in pyuria group than those in control group (all P>?0 . 05 ). No difference was found in the duration of fever before admission between two groups (P>?0 . 05 ). However, pyuria group had longer duration of fever after treatment with immunoglobulin (P?0.05). Conclusion The morbidity of sterile pyuria in KD patients was 22 . 6%. KD patients with sterile pyuria have more intense inlfammatory response, markedly high coagulation condition, and mild or subclinical renal damage.