Objective To investigate the value of serum apolipoprotein M (APOM)concentration in the diagnosis of bacterial pneumonia and its disease severity evaluation.Methods The serum APOM concentration was examined in 250 cases of pneumonia and 50 healthy controls by using the double antibody sandwich ELISA.The ROC curve was used to evaluate the diagnostic value of APOM on bacterial pneumonia and the Bayes discriminant analysis was adopted to construct the discriminant equation based on APOM concentration for evaluating its discriminant significance to the disease severity.Results The serum APOM concentration in the bacterial pneumonia group was (28.9±10.5)μg/mL,which was significantly higher than (19.4±8.93)μg/mL in viral pneu-monia group,(16.4±5.62)μg/mL in the other pneumonia group and(18.1 ±6.15)μg/mL in the healthy controls,the differences had statistical significance(P <0.01).The area under ROC curve for APOM in diagnosing bacterial pneumonia could reach 0.843, showing the good sensitivity and specificity.Moreover,the serum APOM concentration was positively correlated with the disease severity of bacterial pneumonia.The discriminant equation based on APOM concentration as well as sex,age,systolic pressure,body temperature and heart rate could better conduct the classification on the disease severity of bacterial pneumonia and its accuracy could reach to 86.7%.Conclusion The serum APOM concentration has an important clinical value for the diagnosis of bacterial pneumonia and its disease severity evaluation.

Objective To study the changes of Vasodilator-stimulated Phosphoprotein phosphorylation which was dynamically regulated by cell adhesion to extracellular matrix,and to investigate the roles of VASP in the process of tumor cell adhesion and de-adhesion.Methods HeLa cells were divided into four groups: stably adherent cells,cells held in suspension,cells replated onto dishes coated with collagen I and positive control(cells treated with Forskolin).Changes in cell morphology were observed in inverted phase contrast microscope.Phosphorylation of VASP was detected by Western blot,and PKA activity was measured by PKA assay kit.(Results In) untreated,stably adherent cells,VASP was predominantly in the unphosphorylated form; Detachment of cells stimulated PKA activity and induced PKA-dependent phosphorylation of VASP;VASP was phosphorylated gradually following reattachment but returned to base level of phosphorylation during active cell spreading.Conclusion During the process of HeLa cell adhesion and de-adhesion,dynamical changes of VASP from the form of phosphorylation to dephosphorylation via PKA have been observed.

Acid sphingomyelinase (ASMase),an important enzyme for production of ceramide has been reported to be involved in the regulation of cell and organ functions and has been implicated in the development of different diseases such as diabetes,hearty disease,liver and kidney diseases.Diabetic nephropathy is a common complication of diabetes.It was found that ASMase has been closely associated with the development renal disease.Hence,it is of great importance to study the impact of ASMase on renal diseases.

Neonatal hypoxic-ischemic encephalopathy(HIE)is a common disease in neonatal nervous system injury,which often treated by the traditional measures such as three support′s and three symptomatic treatments.Moreover,there is no specific treatment nowadays.The human umbilical cord mesenchymal stem cells(HUC-MSC)are mixture of multifunctional stem cells from neonatal umbilical cord tissues,which has strong abilities of self-renewal and multilineage differentiation potential.HUC-MSC are easy to harvest and have more sources,low immunogenicity and without ethical problems.It can differentiate into neural cells under certain conditions,secrete neurotrophic factors,repair the damaged brain and promote the recovery of cerebral function.Transplantation of HUC-MSC provides a new way for the treatment of HIE.

Muscle-specific microRNAs (myomiRs) are a class of small endogenous non-coding RNAs that expressed specifically in the muscle tissue.By negatively regulating related gene expression at posttranslational level,they participate in a variety of biological processes and affects the occurrence and development of diseases.The occurrence and development of muscle-related diseases,such as chronic obstructive pneumonia disease,hypertrophic cardiomyopathy and so on,induce the expression changes of myomiRs and downstream target genes.The effects of myomiRs on the muscle contraction will affect the development of the disease.This paper will review the biological effects of common myomiRs,such as miR-1,miR-133,miR-206,miR-208 and miR-499 in muscle contraction and relaxation,including striated and non-striated muscle.Better understanding of the effects of myomiRs on the biological effects of muscle contraction and relaxation will provide a new idea for the treatment of muscle-related diseases.

Objective To observe the effects of tanshinone on malignant arrhythmias which occured in rabbit model of myocardi-al infarction,and explore the relationship between tanshinone induced calmodulin(CaM)signal system changes and the incidence of malignant arrhythmias after myocardial infarction.Methods A total of 90 healthy big ear rabbits were selected and randomly divid-ed into 3 groups:myocardial infarction model rabbits(non-intervention group)and myocardial infarction model rabbits with tanshi-none intervention(intervention group)and sham operated rabbits(control group),30 rats in each group.The incidence of malignant arrhythmias,calmodulin(CaM),calmodulin kinaseⅡ(CaMKⅡ),transmural dispersity of repolarization(TDR)and the concentra-tion of Ca2 + in myocardial cells were statistically analysed and compared among the 3 groups.Results The control group did not undergo malignant myocardial infarction,in non-intervention group and intervention group,malignant arrhythmia incidence was higher than that in control group(P <0.05);in non-intervention group,malignant arrhythmia rate(60.00%)was higher than that in intervention group(10%)(P <0.05 ).The CaM concentration in non-intervention and intervention group rabbits were 1.483 and 1.321 times of control group rabbits respectively,the difference was statistically significant(P <0.05);CaM protein levels of non-intervention group was higher than that of intervention group(P <0.05).No statistical significance of CaMKⅡ activity among the 3 groups were observed(P >0.05).Serum Ca2 + concentration in non-intervention group was higher than that in control group and intervention group(P <0.05);serum Ca2 + concentration in control group was lower than that in intervention group(P <0.05).The TDR of intervention group is lower than that of non-intervention group,the two groups′TDR were both higher than that of control group(P <0.05).At single cell level,the intracellular calcium concentration of intervention group was lower than that of non-inter-vention group (P <0.05).Conclusion The relevant signal factors of CaM signal transduction system have implications on malig-nant arrhythmia after myocardial infarction.Tanshinone can inhibit CaM signaling system and play an important role in the preven-tion of malignant arrhythmia after myocardial infarction.

Objective:To establish an HPLC method for the determination of two ingredients in losartan potassium and hydrochlo-rothiazide tablets. Methods: Losartan potassium and hydrochlorothiazide were separated on a Zorbax Eclipse XDB-C8 ( 200 mm × 4. 6 mm, 5 μm)column, the mobile phase consisted of 0. 01 mol·L-1 monopotassium phosphate solution(adjusting pH to 3. 0 with phosphoric acid)-acetonitrile(70∶30)with the flow rate of 1. 0 ml·min-1. The column temperature was set at 35℃, the detection wavelength was 271nm and the injection volume size was 20 μl. Results:The linear range of losartan potassium and hydrochlorothiaz-ide was 39.63-118.89 μg·ml-1(r=0.999 9) and 9.47-28.41 μg·ml-1(r=0.999 6) with the average recovery of 99.4%(RSD=1. 0%,n=9)and 99. 8%(RSD=0. 9%, n=9), respectively. Conclusion: The method is simple, fast, accurate and reproduci-ble, which is suitable for the determination of losartan potassium and hydrochlorothiazide tablets.

Henoch-Schonlein purpura (HSP) is the most common form of childhood autoimmune vascular inflammatory diseases,the incidence rate showed an increasing trend,its pathogenesis has not yet been fully elucidated.Thl7 cells are recently discovered to be a new kind of different from Th1,Th2 effector of CD4+ T cell subsets,Th17/Treg imbalance is closely related to the pathogenesis of HSP.The paper reviewed the related researches.

Ki-67,as one kind of nucleoantigen expressed in proliferated cells, has positive correlation with clinical stage, nodal status and Her-2,while negative correlation with estrogen receptor(ER). High Ki-67 expression influnced by endocrine therapy and chemotherapy indicates good proliferative activity. Ki-67, also as an independent prognostic factor of early breast cancer, provides prognostic information combined with other biologic markers. Ki-67 gives additional information breast cancer prognosis staging.

Kawasaki disease (KD) is an acute, self-limited vasculitis of children and the principal cause of acquired heart disease in children in developed countries. The recent follow-up studies of KD revealed that cardiac sequelae had been observed for many years in a minority of patients with KD. Cardiac sequelae especially the coronary artery aneurysm is the leading cause of ischemic heart disease or even the sudden death.The long-term management of KD was developed in many countries in order to prevent the cardiac events.This paper reviews the long-term prognosis and follow-up of KD.