Objective To investigate the effect of leptin on the secretion of chemokine in THP1 cells and explore its related mechanism, and to provide basis for research on the role of leptin in immune response.Methods The expressions of Ob-Rb and Ob-Rt in THP1 cells were detected by RT-PCR and flow cytometry (FCM).The THP1 cells at logarithm growth phase were selected and randomly divided into blank control group and different concentrations(10,50,100,200μg· L-1 )of leptin groups.Transwell chamber assay was performed to detect the number of invated THP1 cells.The THP1 cells were divided into blank control group and 100μg·L-1 leptin group.Western blotting method was carried out to detect the expressions of p-AKT,p-ERK 1/2,and p-STAT3 in THP1 cells.The THP1 cells were divided into blank control group and 100μg·L-1 leptin group,100μg·L-1 leptin+ DMSO group,100μg·L-1 leptin+50μmol·L-1 AG490 group,100μg·L-1 leptin+10μmol·L-1 LY294002 group and 100μg·L-1 leptin+ 10 mol·L-1 PD980590 group.RT-PCR and Western blotting methods were performed to detect the expression of IL-8.Results Ob-Rb and Ob-Rt were highly expressed in THP1 cells. Compared with blank control group,the number of invated THP1 cells was significantly increased in 50,100,and 200μg·L-1 leptin groups (P<0.05).Compared with blank control group,the expressions of p-STAT3,p-ERK 1/2 and p-AKT in THP1 cells were up-regulated in 100 ug·L-1 leptin group(P<0.05).Compared with blank control group,the mRNA and protein expressions of IL8 in THP1 cells in 50,100,and 200μg·L-1 leptin groups were remarkably increased(P<0.05);compared with 100μg·L-1 leptin group,the expressions of IL-8 in THP1 cells in 100μg·L-1 leptin+10 mol·L-1 PD980590 group and 100μg·L-1 leptin+10μmol·L-1 LY2 94002 group were decreased(P<0.05),while the expression of IL-8 in 100μg·L-1 leptin+ 50μmol·L-1 AG490 group had no change(P>0.05).Conclusion leptin can up-regulate the expression of chemokine in THP1 cells,which might be associated with PI3K-AKT and MAPK/ERK 1/2 signaling pathways.

Objective To study the clinical application effectiveness of clinical nursing path-way management in laparoscopic surgery patients.Methods A total of 120 patients with laparo-scopic surgery were randomly divided into observation group and control group according to random number table method.The control group was used conventional nursing interventions while the ob-servation group received clinical nursing pathway management model based on routine care interven-tions on.After surgery,the preoperative waiting time,length of hospital stay,time to ambulation, anal exhaust time and feeding time were assessed,postoperative complications,satisfaction and mas-tery of health education of the two groups were anlyzed.Results The waiting time before surgery and hospital stay time in the observation group were significantly shorter than that in the control group,hospitalization costs in the observation group was significantly less than the control group, which showed a statistically significant difference(P <0.05).Ambulation time,anal exhaust time and feeding time were significantly shorter than that in the control group,the difference was statis-tically significant (P <0.05).The excellent rate of health education knowledge mastery of patients in the observation group was significantly higher,there was statistically significant difference (P <0.05).Conclusion The clinical application of clinical nursing pathway management is effective in gynecological patients with laparoscopy,so it is worthy of widely application.

Objective To study the clinical application effectiveness of clinical nursing path-way management in laparoscopic surgery patients.Methods A total of 120 patients with laparo-scopic surgery were randomly divided into observation group and control group according to random number table method.The control group was used conventional nursing interventions while the ob-servation group received clinical nursing pathway management model based on routine care interven-tions on.After surgery,the preoperative waiting time,length of hospital stay,time to ambulation, anal exhaust time and feeding time were assessed,postoperative complications,satisfaction and mas-tery of health education of the two groups were anlyzed.Results The waiting time before surgery and hospital stay time in the observation group were significantly shorter than that in the control group,hospitalization costs in the observation group was significantly less than the control group, which showed a statistically significant difference(P <0.05).Ambulation time,anal exhaust time and feeding time were significantly shorter than that in the control group,the difference was statis-tically significant (P <0.05).The excellent rate of health education knowledge mastery of patients in the observation group was significantly higher,there was statistically significant difference (P <0.05).Conclusion The clinical application of clinical nursing pathway management is effective in gynecological patients with laparoscopy,so it is worthy of widely application.

Objective: To establish a laboratory method for detection of Mycobacterium tuberculosis in sputum based on variable number tandem repeat ( VNTR) .Methods: Mycobacterium tuberculosis was tested by VNTR and fluorescent quantitative reverse transcription polymerase chain reaction ( FQ-PCR ) in 130 sputum samples from patients with pulmonary tuberculosis and 200 specimens from patients with other lung diseases.According to the amplification conditions and clinical detection needs, MTUB21, MUTB04, QUB18, QUB26, QUB11b, MIRU31, MIRU10 and MIRU26 were selected as test targets.The results of VNTR and FQ-PCR were compared with Lowenstein-Jensen culture and clinical diagnosis, and analyzed by chi-square test. Results:With the results of L-J culture as the standard, the sensitivity and specificity of VNTR were 93.1%(108/116) and 97.7%(209/214), and those of FQ-PCR were 94 .0% ( 109/116 ) and 96 .7% ( 207/214 ) , respectively; no significant difference was observed between two groups (χ2 =0.352, P =0.569 ).Using the clinical diagnosis as the standard, the sensitivity and specificity of VNTR were 86.9% (113/130) and 100% (200/200), and those of FQ-PCR were 87.7% (114/130) and 99.0%(198/200), respectively;the difference was not statistically significant (χ2 =0.030, P=0.862).In 113 VNTR positive samples, the molecular codes differed from each other in 98.2%samples (111/113);only 2 samples had identical code (5 4 6 8 5 5 3 8 ) .Conclusion:The study suggests that VNTR provides a promising method for diagnosis of clinical tuberculosis.

OBJECTIVETo establish a laboratory method for detection of Mycobacterium tuberculosis in sputum based on variable number tandem repeat (VNTR).

METHODSMycobacterium tuberculosis was tested by VNTR and fluorescent quantitative reverse transcription polymerase chain reaction (FQ-PCR) in 130 sputum samples from patients with pulmonary tuberculosis and 200 specimens from patients with other lung diseases. According to the amplification conditions and clinical detection needs, MTUB21, MUTB04, QUB18, QUB26, QUB11b, MIRU31, MIRU10 and MIRU26 were selected as test targets. The results of VNTR and FQ-PCR were compared with Lowenstein-Jensen culture and clinical diagnosis, and analyzed by chi-square test.

RESULTSWith the results of L-J culture as the standard, the sensitivity and specificity of VNTR were 93.1% (108/116) and 97.7% (209/214), and those of FQ-PCR were 94.0% (109/116) and 96.7% (207/214), respectively; no significant difference was observed between two groups (χ2=0.352, P=0.569). Using the clinical diagnosis as the standard, the sensitivity and specificity of VNTR were 86.9% (113/130) and 100% (200/200), and those of FQ-PCR were 87.7% (114/130) and 99.0% (198/200), respectively; the difference was not statistically significant (χ2=0.030, P=0.862). In 113 VNTR positive samples, the molecular codes differed from each other in 98.2% samples (111/113); only 2 samples had identical code (5-4-6-8-5-5-3-8).

CONCLUSIONThe study suggests that VNTR provides a promising method for diagnosis of clinical tuberculosis.

Humans ; Minisatellite Repeats ; Mycobacterium tuberculosis ; isolation & purification ; Polymerase Chain Reaction ; Sensitivity and Specificity ; Sputum ; microbiology ; Tuberculosis, Pulmonary ; diagnosis