BACKGROUND:At present,there are differences in reporting culture condition of cochleal spiral ganglion cells(SGCs)in circle of primary cell culture.Individual method has poor repeatability,and is not beneficial for practical application.OBJECTIVE:To primary culture and evaluate SGCs of neonatal C57 mice.METHODS:Tissues from cochlear modiolus were acquired from neonatal C57 mice by microanatomy.SGCs from cochlear modiolus were cultured by trypsin digestion,differential velocity adherent technique combined with chemicals.Cell growth was observed by inverted phase contrast microscope and hematoxylin and eosin staining.Immunocytochemistry was employed to classify cell types.RESULTS AND CONCLUSION:After purification,the call body of SGCs from cochlear modiolus was ellipse or triangle,with slender processes in cytoplasm.Nuclei were positive for Nuen(stained brown).Cytoplasm and axon were positive for β3-Tubulin stained brown).These indicated that mouse SGCs were successfully cultured.

Objective To explore the effect of facial nerve decompression via mastoid-epitypanum approach on the treatment of early peripheral traumatic facial paralysis caused by temporal bone fracture.Methods The data of 21 patients with early peripheral traumatic facial paralysis caused by temporal bone fracture in our hospital from October 2011 to June 2016.The facial nerve electrogram and the blink reflex of the injured facial nerve of 21 patients who treated facial nerve decompression via mastoid-epitypanum approach were compared before and after operation.The degree of facial nerve function recovery was evluated by H-B grading method.Results The facial nerve function of all patients had improved in different degrees,85.7% patients recovered to Ⅰ~Ⅱ level.Compared with those before operation,the latency,amplitude and latent period of blink reflex of the ipsilateral facial electroneurography were significantly improved(P<0.05).Conclusion The facial nerve decompression has good effect in the treatment of early peripheral traumatic facial paralysis.

OBJECTIVE: To validate the expression of Cu/ZnSOD and PLUNC protein in chronic sinusitis and nasal polyp tissue by immunohistochemistry. METHOD: Thirty-four nasal polyps, 30 chronic sinusitis and 18 control cases were chosen. All samples were stained with SP immunohistochemical method to examine Cu/ZnSOD and PLUNC protein respectively. RESULT: (1) Interestingly, we found the positive intensity of Cu/ZnSOD and PLUNC protein were the same in one sample, and they were both expressed in nasal epithelium mucosae, glandular epithelium and goblet cells; (2) We calculated that the constituent ratio among nasal polyps, chronic sinusitis and normal nasal mucosa was significant difference (chi2 = 30.689, P<0.01). The positive intensity of nasal polyps was mainly "+", and the positive intensity of chronic sinusitis was mainly "+++", and the positive intensity of normal nasal mucosa was mainly "++". CONCLUSION Both Cu/ZnSOD and PLUNC protein are important part of the innate defense mechanism in nasal mucosa. Once the nasal mucosa is irritated by infectious agents or chemical factors, nasal epithelium mucosae, glandular epithelium and goblet cells will proliferate reactively, and then produce antiinflammatory agents such as Cu/ZnSOD and PLUNC protein to defend inflammation.
Adolescent ; Adult ; Aged ; Case-Control Studies ; Chronic Disease ; Female ; Glycoproteins ; metabolism ; Humans ; Inflammation ; Male ; Middle Aged ; Nasal Mucosa ; metabolism ; pathology ; Nasal Polyps ; metabolism ; pathology ; Phosphoproteins ; metabolism ; Sinusitis ; metabolism ; pathology ; Superoxide Dismutase ; metabolism ; Young Adult