Objective To evaluate the diagnostic and therapeutic status of elderly benign prostatic hyperplasia (BPH).Methods Totally 188 male elderly in outpatient department from January 2010 to March 2012 were enrolled in this study.Medical history was inquired in detail,and prostatic volumes (PV)were measured by abdominal ultrasound,PV≥ 20ml was diagnosed as BPH.Lower urinary tract symptoms (LUTS) was evaluated by international prostate symptom score (IPSS),prostate specific antigen (PSA) was measured.Age≥62 years,PSA≥1.6 μg/L and PV≥31 ml were considered as higher risk of clinical progression of BPH.We investigated the rate of prevalence,awareness and treatment of BPH.PV,IPSS and PSA were compared among different age groups by ANOVA.Logistic regression analysis was made to show the risk factors of BPH.Results Among 188 male elderly,the prevalence rate of BPH was 48.4％,and the total rate of awareness was 41.5％.The rate of awareness resulting from seeing doctor due to LUTS was 10.6％.PV,IPSS and PSA were increased with ageing.Risk factors contributing to BPH were age,hypertension and diabetes.The treatment rate with drugs of 5a-reductase inhibitor was 47.3 ％,however 10 subjects had not been given drugs treatment among 46 patients with higher risk of clinical progression of BPH.Conclusions The prevalence rate of BPH is increased with ageing,the proportion of seeing a doctor with passion is lower because the male elderly have not paid enough attention to LUTS.And doctors have not given drugs intervention to patients with clinical progression BPH in time.We should launch propaganda and education among the elderly patients with hypertension and diabetes and take comprehensive treatment.

Objective To investigate the relationship between benign prostatic hyperplasia (BPH) and urine albumin excretion.Methods A total of 258 male inpatients aged 60 to 70 years were enrolled in this study.According to prostate volume (PV) measured by transrectal ultrasonography,patients were divided into BPH group(PV≥20 ml) and NBPH(non benign prostatic hyperplasia) group(PV＜20 ml).Urine albumin and urine creatinine concentrations were measured and urine albumin/creatinine ratio(UACR) was calculated.Fasting blood glucose(FBG) and fasting insulin(FINS) also were measured,insulin resistance index(HOMA-IR) was calculated based on the formula HOMA-IR=ln(FBG × FINS/22.5).The correlation between PV and other indicators were analyzed by Pearson correlation analysis.The risk factors for BPH were analyzed by logistic regression analysis.Results UACR was significantly higher in BPH group than in NBPH group[(163.6± 69.8) mg/g vs.(73.3 ± 55.6) mg/g,t =4.81,P＜ 0.01],and hypertension duration was longer in BPH group than in NBPH group [(9.2±6.6)years vs.(6.0±3.6)years,t=2.27,P＜0.05].The prevalence rate of BPH and PV were increased along with increasing of urine albumin excretion(x2=4.58,F=4.85,respectively,P＜0.05 or 0.01).Prostate volume was positively correlated with UACR,HOMA-IR,hypertension duration,body mass index(r=0.248,0.229,0.183,0.157,respectively,all P ＜ 0.05).Logistic regression analysis indicated that UACR,HOMA-IR and hypertension duration were the risk factors for BPH(OR=16.348,1.040,0.166,respectively,all P ＜0.05).Conclusions BPH is correlated with UACR,which indicates that there is a close relationship between atherosclerosis and BPH.Insulin resistance probably plays an important role in the progression of arteriosclerosis and BPH.

Objective:To study the phenotypes of T cell in patients with Hashimoto thyrioditis (HT) and to analyze its relationship with the impairment of follicles, so as to provide evidence for clinical diagnosis and treatment. Methods: Totally 136 HT specimens were obtained by fine needle aspiration biopsy (FNAB) and were divided morphologically(L, P, O type). The serum parameters (TPOAb, TGAb, TSH) were tested, and 20 cases with nontoxic goiter were taken as control. Immuno-histochemical assay was performed with 60 specimens and FCM (Flow cytometry ) was used to study 23 specimens. The T cell infiltration and the distribution of CD3+ . CD4+ . and CD8+ T cells were compared between all types of HT tissues. The correlationship between the atrophic degree of follicle with T lymphocytes infiltration and phenotype was analyzed. Results: It was found that L type (n = 58) and P type (n = 28) had more serious follicle atrophy than that of O type (n = 20) (P

Objective To investigate the relationship between glucose excursion and cognitive function in aged type 2 diabetes. Methods A total of 248 aged type 2 diabetes were recruited in this study,all of them wore continuous glucose monitoring system (CGMS) for 3 d to evaluate the glucose excursion including mean amplitude of glycemic excursions (MAGE) which was used for assessing intra-day glycemia variability,and mean daily difference (MODD) which represented day-to-day glycemic variability.During the period of CGMS monitoring,all subjects accepted mini mental status examination (MMSE) for evaluating cognitive function.The relationships of MAGE and MODD with performance on cognitive tests were assessed. Results The over intra-day glucose excursion group had lower MMSE score than the narrow intra-day glucose excursion group[(24.25±6.75)vs.(25.97±0.47),P=0.025].The MMSE score was decreased in over day-to-day glucose excursion group compared with the narrow day-to-day glucose excursion group [(24.21 ± 6.47) vs. (26.01 ± 5.49),P =0.019]. A statistically significant association was observed between MAGE and the score of MMSE(r=- 0.308,P＜0.001),and between MODD and MMSE(r=-0.226,P =0.001).Conclusions Glucose excursion may affect cognitive function in aged type 2 diabetes.The over glucose excursion decreases the score of MMSE.

Objective To evaluate the diagnostic value of endoscopic ultrasound guided fine needle aspiration (EUS-FNA) in combination with flow cytometry (FCM) in lymphoma.Methods From January 2011 to December 2011,the cases of suspicious lymphoma with EUS-FNA examination at Shanghai Ruijin Hospital were retrospectively analyzed.The final diagnosis was according to pathological diagnosis of specimen from the surgery and follow up results.The sensitivity,specificity and accuracy of EUS-FNA combined with FCM in lymphoma diagnosis were initially analyzed.Results A total of 14 suspicious lymphoma patients were collected,eight cases were diagnosed as lymphoma by pathological examination of specimen from the surgery or.tissue from aspiration,four cases were non-lymphoma lesions and two cases still had no final diagnosis.The sensitivity and specificity of FCM alone in lymphoma diagnosis were 4/8 and 4/4 respectively.Six cases of lymphoma were detected by EUS-FNA with FCM.The sensitivity,specificity and accuracy of EUS FNA combined with FCM were 6/8,6/6 and 10/12 respectively.Conclusion EUS-FNA combined with FCM has better diagnostic value in lymphoma,especially for gastrointestinal lymphoma and those surrounding deep lesions.

Objective To investigate the clinical and pathological features of Guillain-Barré syndrome with treatment-related fluctuations (GBS-TRF).Methods Clinical data were obtained from medical records of patients with GBS-TRF during the period 1999 to 2014 in our Hospital.Sural nerve specimens were collected and summarized retrospectively (two cases).Results Eight of 868 cases with GBS had at least one TRF including three chronic hepatitis B patients.The onset of disease was ranged in age from six to 63 years, averaging 34 years.It is more common in men than in women in a ratio of seven to one.Triggering infections occurred in three patients.The initial symptom included weakness of the lower limbs (five cases) and upper extremities (three cases).Sensory symptom was presented in six patients.Five patients had associated respiratory paralysis.None of them had cranial nerve palsy or autonomic dysfunction.The examination of cerebrospinal fluid showed protein and cell separation.Five patients had two attacks, one had three attacks and two had six attacks.The interval between attacks ranged between 14 days and 46 days (mean 23 days).The striking pathologic finding was the presence of sectional selective nerve fiber degeneration (SNFD) with evidence of demyelination.Conclusions Patients with GBS-TRF show similar onset age, preceding infection, cerebrospinal fluid findings, and electrophysiologic characteristics comparing to patients with GBS,while there are more male patients than female patients.SNFD found in sural nerve biopsy reveals ischemic neuropathy, which predicts that injury of arterioles might play an important role in the pathogenesis of GBS-TRF.

Objective To investigate the clinical and pathological features of Guillain-Barré syndrome with treatment-related fluctuations ( GBS-TRF ).Methods Clinical data were obtained from medical records of patients with GBS-TRF during the period 1999 to 2014 in our Hospital.Sural nerve specimens were collected and summarized retrospectively ( two cases ).Results Eight of 868 cases with GBS had at least one TRF including three chronic hepatitis B patients.The onset of disease was ranged in age from six to 63 years, averaging 34 years.It is more common in men than in women in a ratio of seven:one.Triggering infections occurred in three patients.The initial symptom included weakness of the lower limbs ( five cases ) and upper extremities ( three cases ).Sensory symptom was presented in six patients.Five patients had associated respiratory paralysis.None of them had cranial nerve palsy or autonomic dysfunction.Five patients had two attacks , one had three attacks and two had six attacks.The interval between attacks ranged between 14 days and 46 days ( mean 23 days ).The striking pathologic finding was the presence of sectional selective nerve fiber degeneration ( SNFD ) with evidence of demyelination.Conclusions Patients with GBS-TRF shows similar onset age , preceding infection , cerebrospinal fluid findings, and electrophysiologic characteristics comparing to patients with GBS ,while there are more male patients than female patients.SNFD found in sural nerve biopsy reveals ischemic neuropathy , which predicts that injury of arterioles might play an important role in the pathogenesis of GBS -TRF.

Objective To assess the theoretical knowledge and practical skills of parasitic diseases among technicians from disease control and prevention institutions. Methods The Assessment on National Parasitic Disease Control and Prevention Techniques was organized in September,2015. Together,124 subjects from disease control and prevention institutions at prov-ince,prefecture or county levels in 31 provinces joined the assessment. A database was built consisting of subjects'basic infor-mation and assessment scores. Statistical analysis was used to analyze the scores by gender,age,professional title,institutions and places of participants. Results The average total score of all the subjects was 123.3,with a passing rate of 57.3%. The av-erage scores of male subjects(48 subjects)and female subjects(76 subjects)were 125.9 and 121.7 respectively;the average scores of the subjects aged under 30 years(57 subjects),between 30 and 40 years(61 subjects)and above 40 years(6 sub-jects)were 119.6,128.1 and 111.2 respectively;the average scores of persons with junior(94 subjects),intermediate(28 sub-jects)and senior(2 subjects)professional titles were 119.2,135.9 and 140.5 respectively. The average theoretical assessment score of all the subjects was 61.9,with a passing rate of 62.9%. The average practical skill assessment score of all the subjects was 61.4,with a passing rate of 58.1%. Conclusions The theoretical assessment results range widely. The theoretical knowl-edge results of technicians from disease control and prevention institutions are low in general. Therefore ,the specific training based on daily work needs to be enhanced.

Objective To investigate the rehabilitation of pelvis disarticulation amputees.Methods A case report.Results and Conclusion Rehabilitation engineering,assisted with physical therapy,can rebuild hemicorporectomy amputees' standing and walking ability,make them look as normal as possible,help them return to the society.

Un-transfected rabbit corneal endothelial cells (RCECs) were cultivated, using chitosan blend membrane 4ha (chitosan-hyaluronic acid), 631ha (chitosan-hyaluronic acid) and 631s (chitosan-chondroitine sulfate) as scaffold carriers. Their biocompatibilities were studied in regard to cell adherence, morphological changes, growth status and monolayer forming abilities. The results indicated that RCECs cultivated on 4ha and 631ha carriers tended to be aggregated and even desquamated to some extent in local areas, and even more severely on 631ha carrier. And the RCECs cultivated on 631ha carrier could form almost a monolayer 48h later, and those on 4ha carrier could not. Contrarily, the RCECs cultivated on 631s carrier were evenly distributed and were in good status of growth with a good adherence and fibroblast-like morphology which could form almost a monolayer 48h later. And a complete monolayer was formed and was tightly attached to the 631s carrier 72h later. From the above results, it can be concluded that 631s carrier is most probably an ideal scaffold carrier for RCEC cultivation. 631s carrier may have the potential for use in the development of tissue-engineered rabbit corneal endothelium.
Animals ; Biocompatible Materials ; chemistry ; Cell Adhesion ; Cell Culture Techniques ; Cell Line ; Cells, Cultured ; Chitosan ; chemistry ; Endothelium, Corneal ; cytology ; Hyaluronic Acid ; chemistry ; Membranes, Artificial ; Rabbits ; Tissue Engineering ; methods ; Tissue Scaffolds ; chemistry