Objective Constructing the dendritic cell (DC) vaccine loading HPV16L1 antigen,and evaluating the specific humoral and cellular immune responses induced by DC-L1.Methods The mature dendritic cells (DCs) were induced in vitro from bone marrow precursors by co-culturing with cytokines cocktail,and then transduced with a recombinant adenovirus harboring the HPV16L1 gene to obtain the DC-L1 vaccine.C57BL/6 mouse were immunized intramuscularly by single dose of DC-L1 and recombinant adenovirus vaccine Adv-HPV16L1 respectively.The specific humoral and cellular immune responses were determined by quantitative ELISA and ELISPOT at different time points.Results DC-L1 cells exhibited typical morphological features of dendritic cells,and the expression of HPV16L1 can be detected by western blot.Both DC-L1 and Adv-HPV16L1 can induce specific cellular immune response,while with distinct kinetics.Single dose of Adv-HPV16L1 induced responses immediately,and attained the peak level at the third week after immunization,then diminished gradually.While responses induced by DC-L1 elevated slowly,and eventually exceeded that of recombinant adenovirus group at the fifth week of postimmunization.As to humoral immunity,both DC-L1 and Adv-HPV16L1 vaccines can induce specific humoral immune reponses,and with similar kinetics.Conclusion The DC-L1 vaccine was constructed successfully.The DC-L1 vaccine can induce high levels of HPV16L1 specific humoral and cellular immune response.