0.05) . Operation time and bleeding in group A were significantly less than those in group B( P

Objective To compare the brainstem auditory evoked potential (BAEP) in patients with different kinds of brainstem disorders, so as to offer a significative electrophysiological means for diagnosis and differential diagnosis of different kinds of brainstem disorders. Methods BAEP was recorded from 80 cases of vertebrobasilar arterial insufficiency, 56 cases of acoustic neuroma and 47 cases of chronic cerebellar degeneration and compared with that from 50 healthy people. Results The abnormal rates of the three different case groups were 73.75%, 94.61%, 89.36%, respectively. Each group of patients demonstrated their own characteristics with regard to the BAEP. Conclusion Each of the three brainstem disorders has its own characteristics with BAEP.

The investigator raised the possibility that the authors hadn't conducted the research. Therefore, the entire article has been retracted in accordance with this journal's policy and Editorial decision.

DRG-based prospective payment system ( DRG-PPS ) is one type of mature inpatient care payment mechanisms in most countries and pilot projects would be introduced to China in 2017. This article provides an overview of the previous typical DRG-PPS reform initiatives on provincial, municipal and county level respectively. It also summa-rizes the characteristics of the reform programs, describes the diversities of policy implementation, and discusses the suc-cessful experience and implications of the initiatives. The paper puts forward some suggestions on the upcoming DRG-PPS pilot projects based on the progress and problems of the initiatives, to promote the DRG-PPS reform in China.

PURPOSE: Protein phosphatase 4 regulatory subunit 1 (PP4R1), as an interaction partner of the catalytic serine/threonine-protein phosphatase 4 catalytic subunit has been shown to involve in cellular processes and nuclear factor kappaB signaling. However, the functions of PP4R1 in human breast cancers remain unclear. This study is designed to explore the effect of PP4R1 knockdown on the biological characteristics of breast cancer cells. METHODS: A lentivirus-mediated short hairpin RNA (shRNA) was designed to knockdown the expression of PP4R1 in ZR-75-30 breast cancer cells. The efficiency of lentivirus-mediated shRNA infection was determined using fluorescence microscopy to observe lentivirus-mediated green fluorescent protein expression and confirmed to be over 80%. PP4R1 expression in infected ZR-75-30 cells was detected by quantitative real-time polymerase chain reaction and western blot analysis. Cell proliferation and colony formation ability were measured by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) assay and colony formation assay, respectively. Flow cytometry was used to measure cell cycle progression and cell apoptosis. In addition, apoptosis makers, including poly-ADP-ribose polymerase (PARP) and caspase-3, were investigated in PP4R1-silenced ZR-75-30 cells by western blot assay. RESULTS: We successfully constructed lentivirus-mediated shRNA to target PP4R1 in ZR-75-30 cells. MTT assay and colony formation assay showed the loss of PP4R1 suppressed the proliferation of ZR-75-30 cells. Flow cytometry analysis indicated cell cycle arrest and increased cell apoptosis in PP4R1 knockdown cells. Further, the apoptosis response in cells depleted of PP4R1 was illustrated by downregulation of PARP and upregulation of caspase-3. CONCLUSION: Our results suggest that PP4R1 could promote breast cancer cell proliferation and might play a vital role in breast cancer occurrence.
Apoptosis ; Blotting, Western ; Breast Neoplasms* ; Breast* ; Caspase 3 ; Catalytic Domain ; Cell Cycle ; Cell Cycle Checkpoints ; Cell Proliferation ; Down-Regulation ; Flow Cytometry ; Humans ; Microscopy, Fluorescence ; Population Characteristics ; Real-Time Polymerase Chain Reaction ; RNA* ; RNA, Small Interfering ; Up-Regulation

This article was initially published on the Journal of Breast Cancer with a misspelled name of the second author.
Breast Neoplasms* ; Breast* ; RNA*