Objective To explore the protective effect of hesperidin pretreatment on concanavalin A (Con A)-induced acute liver injury and the effect on expression of TNF-α and IFN-γ. Methods Seventy-two SPF C57BL/ 6 mice were randomly divided into three groups: normal control group, model control group and hesperidin group. Acute liver injury model was established by injected with Con A. The hesperidin group was treated intragastrically with 1 000 mg·kg-1 hesperidin for 10 days. Model control group was treated intragastrically with the same volume of 0. 5% of sodium carboxymethyl cellulose. Serum levels of alanine aminotransferase (ALT) and aspartate aminotransferase ( AST) were measured. Pathological changes in hepatic tissue were observed under microscope. The expression of TNF-α and IFN-γ mRNAs in hepatic tissue was measured by reverse transcription polymerase chain reaction ( RT-PCR). The contents of TNF-α and IFN-γ in serum were detected by ELISA. Results Compared with model control group, the contents of ALT and AST in serum were significantly decreased (P<0. 01) in hesperidin group. Pathological changes in hepatic tissue were markedly improved. The expression of TNF-α and IFN-γ in the hepatic tissues and serum were significantly downregulated (P<0. 01). The concentrations of TNF-α and IFN-γ in hesperidin group were (717. 05±205. 22) and(611. 06±92. 82)pg·mL-1 in 2 h,(811. 56±167. 47)and(786. 19±215. 44)pg·mL-1 in 6 h. Compared with model control group, the expressions of TNF-α and IFN-γ in the hesperidin group were significantly downregulated (P<0. 01). But there was no significant difference between hesperidin group and model control group in 6 h after treated with Con A(P>0. 05). Conclusion Hesperidin pretreatment protects mice from Con A-induced acute liver injury possibly by inhibiting the expression of TNF-α and IFN-γ in the liver of mice.

Objective To investigate the prognosis and its influencing factors for patients with hepatocellular carcinoma (HCC) complicated with portal hypertension (PHT) after radiofrequency ablation (RFA).Methods Clinical data of 223 patients with HCC who were treated with RFA for the first time in the Affiliated Hospital of Qingdao University from January 2011 to December 2015 were retrospectively analyzed. There were 168 males and 55 females, aged from 31 to 80 years old with the median of 57 years old. The patients were divided into PHT group and non PHT group according to whether they were complicated with PHT or not. The informed consents of all patients were obtained and the local ethical committee approval was received. RFA was performed under the guidance of ultrasound or CT, and the patients were followed up after operation. Survival analysis was conducted by Kaplan-Meier and Log-rank tests, and multivariate analysis was conducted by Cox hazard regression model.Results The median follow-up time of the patients was 38(8-79) months. The Results of Kaplan-Meier analysis showed that the 1-, 2-, 5-year tumor free survival rates of PHT group were 85.8％, 67.7％, 43.8％ respectively, and those of non PHT group were 90.0％, 79.9％ and 62.1％ correspondingly, and significant difference was observed (χ2=8.357, P<0.05). The 1-, 2-, 5-year overall survival rates of PHT group were 100.0％, 93.7％ and 68.9％ respectively, and those of non PHT group were 99.1％, 97.2％ and 91.7％ correspondingly, and significant difference was observed (χ2=6.013, P<0.05). AFP>100 μg/L was an independent risk factor for the tumor-free survival of patients with HCC complicated with PHT after RFA treatment (HR=1.824, P<0.05). Preoperative alcoholism was an independent risk factor for the overall survival of patients with HCC complicated with PHT after RFA treatment (HR=2.724, P<0.05). Conclusions The tumor-free survival rate and overall survival rate of patients with PHT after RFA treatment are significantly lower than those without PHT. Preoperative AFP elevation is an independent risk factor for tumor-free survival, and preoperative alcoholism is an independent risk factor for overall survival.