Humans ; Infant ; Job Syndrome ; diagnosis ; immunology ; therapy ; Male

Objective:To observe the therapeutic effect of ‘Three Steps to Regulate Qi Method’ tuina for infantile cough.
Methods:Fifty-one children with cough were enrolled and received ‘Three Steps to Regulate Qi Method’ tuina, once a day for 5 d. The effects were observed after 5 treatments.
Results:There was no dropout case during the process. Among the 51 infantile patients, 37 cases were cured, 14 cases were improved, and the total effective rate was 100%.
Conclusion:'Three Steps to Regulate Qi Method’ tuina is an effective, simple and convenient method for infatile cough and worth spreading in clinical application.

Aim To investigate the role and mechanism of NLRP3 on hypolipidemic effect and anti-inflammative effect of apigenin. Methods Triton-WR 1339-induced hyperlipidemia was applied to wide type C57BL/6 and NLRP3"'" mice, which was treated with apigenin of 6.25 mg • kg"1 • day"1 for five days. Blood and liver tissueswere collected for detecting TC, TG, HDL, LDL, IL-1B, IL-6, MCP-1 and the liver underwent HE staining. The expressions of NLRP3, I L 4, ASC, CD36, CYP7A1 and FGF21 were tested using RT-qPCR. Results Compared with NLRP3 "'" model group, serum contents of TC, TG, HDL, LDL, IL-1B, IL-6, MCP-1 were reduced in NLPR3"'" treated with apigenin of 6. 25 mg • kg"1 (P < 0. 05). The percentage of hepatic steatosis wasdown-regulated by apigenin in pathogenesis observation. However, all these phenotype changes were not observed in WT mice treated with apigenin. Moreover, up-regulation of CD36 and vLDLR and down-regualtion of ASC and IL-4 were founded in both WT and NLRP3"'" model group (P < 0. 05), while down-regulation of FGF21 and up-regulation of CYP7A1 were only seen in NLRP3"/ _ model group but not in WT group. Conclusions Knockout of NLRP3 enhances hypolipidemic effect and anti-inflammative effect of apigenin in triton-1339 IP-induced hyperlipidemia mice, which may be associated with apigenin-regulated FGF21/CYP7A1 pathway without NLRP3 inflammasome interruption.

Objective To prepare and characterize nanoparticles in-loaded one-way release-controlled chitosan membrane,and to explore the release-controlled rule of the film in vitro.Methods The chitosan nanoparticles were prepared by inverse crosslinking-emulsion method.The one-way release-controlled membrane was prepared by a casting method.Transmission electron microscope (TEM) was used to evaluate the morphological properties and particle size analyzer was used to analyze particle size distribution.The morphology of the membrane was inspected through scanning electron microscope (SEM).MTT assay was applied to determine the biological safety of chitosan nanoparticles.The distribution of the nanoparticles was observed by fluorescence microscope.The in vitro release studies were adopted to evaluate the release-controlled rule.Results The four kinds of nanoparticles had spherical shapes and uniform particle size.The size of the hyaluronic acid-coated chitosan nanoparticle was (255.40±39.10) nm.Hyaluronic acid-coated chitosan nanoparticles showed the best property of sustained release and biocompatibility.The membrane had a loose inner layer and a dense outer layer,and the distribution of the nanoparticles was uniform in the inner layer of the membrane.The release of protein from membrane was unidirectional and the membrane displayed good controlled release property.Conclusions The nanoparticles in-loaded one-way release-controlled chitosan membrane presents good one-way sustained release performance.It is potentially useful in delivery system of growth factors.

Animals ; Astragalus Plant ; Cardiomyopathies ; Myocardial Ischemia ; Myocardium ; Rats

AIM: To establish the quality standard for Qidan Granules(Radix et Rhizoma Salviae Miltiorrhizae,Radix Astragali,Myrrha,Rhizoma Curcumae;Faeces Togopteri). METHODS: Myrrh,Rhizoma Curcumae and Faeces Trogopteri in Qidan Granules were identified by TLC.The content of tanshinone Ⅱ_A was determined by HPLC.The astragaloside content was determined by HPLC-ELSD. RESULTS: Myrrh,Rhizoma Curcumae and Faeces Trogopteri could be identified by TLC.There was a good linear relationship between the peak area and quantity of tanshinone Ⅱ_A at the range of 2.98-953.6 ng,r=0.999 9,and the recovery was 100.6%,RSD was(0.5%.) Also,there was a good natural logarithm linear relationship between the peak area and quantity of astragaloside at the range of 2.05-40.92 ?g,r=0.999 8,and the recovery was 102.0%,RSD was 1.8%.(CONCLUSION:) The method can be used to set up the quality standard for Qidan Granules.

Objective:To investigate the current status of cognitive frailty among elderly patients in Urumqi and to identify its influencing factors.Methods:From March to December 2019, the elderly from 3 tertiary hospitals′ geriatrics centers in Urumqi were recruited using the general information questionnaire, FRAIL Scale, Montreal Cognitive Assessment and Clinical Dementia Rating.Results:A total of 1 006 elderly patients were surveyed, among which, 131(13.0%) cases were deemed to have developed cognitive frailty. The results of multivariate logistic regression analysis showed that age, depression, Instrumental Activities of Daily Living Scale score and diabetes were influencing factors of cognitive frailty ( P<0.001). Conclusion:The prevalence rate of cognitive frailty in elderly patients is relatively high. Medical staff should attach great importance to the assessment of cognitive frailty in elderly patients and take targeted intervention in time to prevent, slow down or reverse the onset and development of cognitive frailty.

Abstract: Objective　To understand the pollution characteristics and sources of PM2.5 in the atmosphere during heating and non-heating periods in Urumqi City in 2021, and provide scientific basis for pollution control during different periods. Method A total of 188 air samples were collected from area A and area B of Urumqi City, and 12 metal elements and 5 water-soluble ions were quantitatively analyzed, and the pollution sources were analyzed by enrichment factor method and principal component analysis method. Results In 2021, the mass concentrations of PM2.5 in areas A and B of Urumqi were 45.0 (20.0, 158) µg/m3 and 28.0 (17.5, 66.0) µg/m3, respectively, with statistically significant difference (Z=-2.870, P<0.05). During the heating period, the concentrations were 110 (68.0, 250) µg/m3 and 61.0 (31.0, 88.0) µg/m3, respectively, with no statistically significant difference (Z=-3.822, P<0.01). During the non-heating period, the concentrations were 18.0 (13.0, 22.3) µg/m3 and 18.0 (12.8, 22.0) µg/m3, respectively, with no statistically significant difference (Z=-0.596, P>0.05). The SNA (the sum of SO42-, NO3-and NH4+) accounted for 71.7% and 23.4% of PM2.5 in A area during heating and non-heating periods, respectively, with statistically significant difference (Z=-8.057, P<0.01); the corresponding proportions in B area were 60.7% and 24.9%, with statistically significant difference (Z=-6.672, P<0.01). During the heating and non-heating periods, the ratios of NO3-/SO42-are 0.63 and 0.54 in A area were 0.63 and 0.54, respectively, with statistically significant difference (Z=-2.382, P<0.05); and the corresponding ratios in B area were 0.72 and 0.53, respectively, with statistically significant difference (Z=-3.182, P<0.05). The ratio of NO3- to SO42- was less than 1 in both heating and non-heating periods in the two areas. and the correlation between five water-soluble ions was significant (P<0.05). The correlation coefficient between NH4+ and SO42-, NO3-and Cl- in A and B areas during heating periods were all >0.9, indicating that NH4+and SO42-, NO3- and Cl- bind in (NH4)2SO4, NH4HSO4, NH4NO3, and NH4Cl. During non-heating periods, the correlation between NH4+ and each ion was slightly lower. During heating periods in area A, Sb, As, Cd, Pb, and Tl were severely enriched (EF>100). During non-heating periods in the same area, As, Cd, Pb, Tl, and Hg were severely enriched (EF>100). During heating periods in area B, Sb, As, Cd, Pb, and Hg were severely enriched (EF>100), and during non-heating periods in the same area, Sb, Cd, and Hg were severely enriched (EF>100). Coal emission, photochemical secondary pollution, motor vehicle exhaust, dust and industrial pollution were the main sources of PM2.5 pollution in the two areas, and the contribution rate of fixed sources was higher than that of mobile sources. Conclusion In 2021, the mass concentration of PM2.5, water-soluble ions and metal elements in Urumqi City were higher in area A than area B, the heating period was higher than the non-heating period, the excess rate of area A was higher than that in area B, and the contribution rate of fixed air pollution was greater than that of mobile sources.