High-intensive phototherapy is a therapy recommended American Academy of Pediatrics for neonatal hyperbilirubinemia.The new bule light emitting diode (LED) so far has become an ideal phototherapy equipment for its proper Light intense.As the increasing appliance of the high-intensive phototherapy,its clinical value and safety are attracting more and more attention.This review is aimed at summarizing the clinical efficacy and safety of the new bule light emitting diode(LED) in the treatment of neonatal hyperbilirubinem.

Child, Preschool ; Foreign Bodies ; surgery ; Humans ; Intestines ; Laparoscopy ; methods

Objective To establish a HPLC method for the determination of baicalin and berberine hydrochloride in Com-pound Qinbo Granules. Methods The gradient elution was performed on a Diamonsil C18 column using method-water (0. 05 % phosphate acid and 0. 1% trithylamine) as mobile phase, and the detection wavelength was 276 nm. Results The linear calibration curve of baicalin was obtained in the range of 10. 08～201.6 mg·L-1 with an average recovery of 98. 1% (RSD=1.11%). The linear calibration curve of berberine hydrochloride was in the range of 4. 08～81.6 mg·L-1 with an average re-covery of 97.9 % (RSD=1.24 %). Conclusion The method is simple and reproducible. It can be used for the quality con-trol of Compound Qinbo Granules.

OBJECTIVE: To develop a kit for detection of DNA of Fritillaria cirrhosa D. Don and optimize its components as well as process protocols, in order to set up a simple, rapid molecular biology method for identification of Fritillaria cirrhosa D. Don. METHODS: All genomic DNA of Fritillaria cirrhosa D. Don was extracted by kit assay and pharmacopoeia method recorded in the expanded supplement of China Pharmacopoeia 2010, respectively; ultraviolet spectrophotometer was used to measure the quantity of extracted DNA; PCR amplification and restriction fragment length polymorphism analysis (RFLP) were carried out to identify the authentication of Fritillaria cirrhosa D. Don. RESULTS: The maximum value of genomic DNA extracted by pharmacopoeia method was (1.57±0.05) (OD260/OD280) and (1.73±0.10) by kit assay. The PCR amplification showed a single band over 300 bp, while the RFLP showed two distinct bands between 100 and 250 bp in agarose electrophoresis. CONCLUSION: The data demonstrated that the kit assay was better than the pharmacopoeia method, especially in the extraction quantity and DNA purity of Fritillaria cirrhosa D. Don nicleic acid; the PCR and RFLP results showed that the kit assay was consistent with pharmacopoeia method. The detection kit has good specificity, high sensitivity and good stability, so it is suitable for the rapid detection of Fritillaria cirrhosa D. Don.

Animals ; Cricetinae ; DNA Damage ; physiology ; Genes, p53 ; physiology ; Humans ; Mice ; Signal Transduction ; physiology

At present, filtration surgery remains an important treatment of glaucoma, and filtering bleb fibrosis is the main cause for treatment failure. Filtering bleb fibrosis is a common fiber hyperplastic disease, and it relates to the activation and proliferation of fibroblasts and the excessive production of extracellular matrix ( ECM) such as collagen protein. The most frequently-used drugs for filtering bleb fibrosis in clinic are 5-fluoro-2,4 (1h, 3h) pyrimidinedione ( 5 - Fu ) and mitomycin ( MMC ) . Although they are effective in some degree, they also have some serious side effects which restrict their clinical use. Triptolide ( TPL) is a major active component of the medicinal plant, tripterygium wilfordii hook. f. ( TWHF) . TPL has multiple pharmacological activities including immunosuppressive, anti- inflammatory, anti- cancer and anti-fertility activity. Reviewing related literatures published in recent ten years, we confirmed that TPL seemed to possess a pharmacological activity in treating filtering bleb fibrosis. Since it has three major functions:1. inhibit the activation and proliferation of fibroblasts and the excessive production of collagen protein; 2. alleviate the inflammatory reaction after surgical wound to suppress fibrous scar formation; 3. TPL has a protective effect on retinal ganglion cells ( RGCs ) . We further find that TPL's anti-fibrosis activity mainly results from that it inhibits TGF-β/Smad,NF-κB and PI3K/AKT signal transduction pathway. This comprehensive analysis about the feasibility of Triptolide's role in treating filtering bleb fibrosis after the filtration surgery of glaucoma can help us develop new drugs for filtering bleb fibrosis and exploit TPL's clinical value on some level.

Adult ; Butadienes ; adverse effects ; Chemical Industry ; Environmental Monitoring ; Humans ; Middle Aged ; Occupational Exposure ; adverse effects ; Occupational Health ; Physical Examination ; Young Adult

OBJECTIVE: To optimize DNA extraction methods and PCR reaction parameters, and develop an excellent and accurate rapid detection reagent for Fetus Cervi. METHODS: The DNA of Fetus Cervi was extracted by the modified salting method, modified SDS method A and modified SDS method B. Four DNA polymerase were chosen from the market and compared with each other. The annealing temperature and annealing time were optimized by classical control variable method and intersected experiment. A rapid detection reagent of Fetus Cervi was developed and then evaluated. RESULTS: The A260 /A280 ratio of the DNA extracted by modified SDS method B was (1.74 ± 0.05), and the mass concentration was (0.250 ± 0.005) μg•L -1. With high fidelity Taq DNA polymerase, the PCR product concentration could reach (0.185 ± 0.005) μg•L-1. Through these experiments, the annealing temperature was set at 58 ℃ and the annealing time was 30 s. The rapid detection reagent course was established to quickly and accurately identify Fetus Cervi and their artefacts, with one clear and bright band at 563 bp. CONCLUSION: The rapid detection reagent of Fetus Cervi combines the optimal DNA extraction method and the optimal PCR reaction parameters, and can accomplish the identification of Fetus Cervi and its pseudo-products with high accuracy and specificity.

0.05).Conclusion There may be no significant difference in sensory block,motor block and prevalence of adverse effects between injection rates of 0.27 mL/s and 0.04 mL/s in spinal anesthesia with 20 mg isobaric ropivacaine.