As a subset of Inhibitory T cells,CD4+ CD25+ Foxp3+ regulatory T (Treg) cells could strongly inhibit the activation,proliferation and function of effector T lymphocytes cells.Treg cells play an important role in downregulate autoimmune and mediating transplantation tolerance.Treg cells concerntrations could be markedly increased expression in tumor issues and peripheral blood of lung cancer.The clinical staging of lung cancer and lung cancer pathological types.Treg cells are important for lung cancer diagnosis and treatment.

Acute Disease ; Adult ; Female ; Humans ; Male ; Organometallic Compounds ; poisoning ; Poisoning ; therapy

Traditional Chinese medicineZhizi is the dry ripe fruit of Gardenia jasminoides Ellis, which belongs to the family of Rubiaceae. The main effective constituents of gardenia include iridoid, gardenia yellow pigment and organic acid. Modern pharmaco-logical studies showed that the main pharmacological effects of gardenia included anti-inflammatory, antipyretic and analgesic effects, liver protection, cholagogue, antilipidemic effect, antithrombus, nerve protection and anti-Alzheimer' s disease. In this paper, the ef-fective constituents and pharmacological effects of Gardenia jasminoides Ellis were reviewed to provide available information for further research.

OBJECTIVE:To provide reference for the development of online drugstores in China based on mobile port. METH-ODS:The development of online drugstores in China based on mobile port was analyzed by SWOT(strengths,weaknesses,oppor-tunities,threats)analysis to put forward suggestions. RESULTS & CONCLUSIONS:Online drugstores based mobile port included vertical management in which pharmaceutical E-commerce manager directly participate,non-vertical management which mainly pro-vide information inquiry and price comparison. It has advantage in breaking through time and space obstacle,optimizing informa-tion display,integrating consumer’s information accurately,improving human efficiency,etc. They are weak in timeliness,busi-ness scope and sale function,the quality of pharmaceutical care,user activity,etc. They have the opportunities as annual increase of mobile E-commerce market scale and huge market development potential;they also face various threats,such as the inconformi-ty between main users of mobile port and main purchasers,consumer low-trust,health-care polity barrier breakthrough failure,the lack of legal regulations for medical and pharmaceutical software,etc. It is suggested that companies operate further accurately and the government speed up the formulation and implementation of laws and regulations.

AIM:To discuss the causes and therapeutic measures of Descemet's membrane detachment during phacoemulsification ( Phaco) .
METHODS:A retrospective analysis of 997 patients who had operations of Phaco and intraocular lens ( IOL ) implantation at the eastern part of Yangzhou No. 1 People's Hospital during 2007-2012. The treatment and prognosis of 21 patients who had the complication of Descemet's membrane detachment during the surgery were observed.
RESULTS: The vision acuity of all the 21 patients had improved to different extent after therapy, no one had the decompensation of corneal endothelium.
CONCLUSION:Early detection and treatment is decisive for the vision acuity of the patients who had the Descemet's membrane detachment.

Child ; Clinical Laboratory Techniques ; Humans ; Microbiological Techniques ; Viral Load ; Virus Cultivation ; Virus Diseases ; diagnosis

BACKGROUND:The studies about biomechanics of total knee arthroplasty were more, but the research on finite element analysis of the effect of total knee arthroplasty on proximal tibial reconstruction was not much.
OBJECTIVE:To study the effect of total knee arthroplasty on proximal tibial reconstruction using finite element analysis.
METHODS:We designed knee models of two materials:one was functional y graded material models of col agen and titanium composite materials;another was titanium al oy material model. We created two prosthesis material tibia-knee prosthesis model, and analyzed bone density distribution of proximal tibia, the maximum stress value of the medial tibia and the lateral tibia, and the maximum stress value of medial tibia and prosthesis junction, as wel as lateral tibia and prosthesis junction after knee arthroplasty.
RESULTS AND CONCLUSION:(1) Bone density of proximal tibia after total knee arthroplasty:The bone density of the knee prosthesis handle with medial and lateral tibia at the junction of tibial plateau was decreased than that of the initial state. Stress shielding effect was lower in col agen and titanium composite materials of functional y graded material models than that of titanium al oy material model. The bone density of shank end of the tibial prosthesis was obviously increased compared with the medial and lateral tibia, showing stress concentration. (2) The maximum stress value of the medial and the lateral tibia:The maximum stress value of the medial and the lateral tibia in col agen and titanium composite models was lower than that of titanium al oy material model. The maximum stress values of the lateral tibia of col agen and titanium composite models and titanium al oy material model were lower than that of medial tibia. (3) The maximum stress of medial tibia, lateral tibia and prosthesis junction:The stress distribution of col agen and titanium composite models was relatively uniform. The stress distribution of titanium al oy material model showed fluctuation. Stress concentration appeared in pal ets and prosthesis junction and the shank end of the prosthesis. The maximum stress values of lateral tibia and prosthesis junction of col agen and titanium composite models and titanium al oy material model were lower than that of medial tibia and prosthesis junction. (4) These findings showed that knee prosthesis has a certain stress shielding effect on tibia after total knee arthroplasty. The stress shielding effect of col agen and titanium composite material prosthesis was lower than titanium al oy material prosthesis, and could improve tibial incurred stress.

Objective: To investigate the inhibition of luteoloside on the proliferation of human esophageal carcinoma cell line Eca109 and its mechanism. Methods: MTT assay was used for detecting the influence of luteoloside at different concentration on the proliferation of Eca109. The morphological changes of cells were observed under inverted microscope. The cell cycle and apoptosis were detected by flow cytometry. RT-PCR was used to detect the mRNA expression of cyclin D1, survivin, and c-myc genes. Results: The results of MTT assay showed different doses (80, 120, 160, 200, and 240 μmol/L) of luteoloside could inhibit the cell proliferation of Eca109 cells in a dose-response manner. Luteoloside could also change the morphological characteristics of cells, reduce the cell size, and separate from peripheral cells. Treated with 240 μmol/L luteoloside, the cells were sprouting and some of them developed multiple pseudopodia-like protrusions. Treated with luteoloside (160 and 240 μmol/L), the cell cycle of Eca109 cells was blocked in G2/M phase and apoptosis was induced (P < 0.05). Compared with the control group, the gene expression of cyclin D1, survivin, and c-myc was decreased after treated with 240 μmol/L luteoloside for 48 h (P < 0.05). Conclusion: Luteoloside could significantly inhibit the proliferation of Eca109 cells by changing the cell cycle and inducing the apoptosis. Moreover, it could decrease the mRNA expression of relative genes.

Objective:This study aims to investigate the expression pattern of miR-512-3p in breast cancer and noncancerous paired specimens as well as the effects of miR-512-3p on the proliferation, apoptosis, cell cycle, and cloning of MD-MBA-231 breast cancer cells. The study also aims to identify the miR-512-3p target gene. Methods:Reverse transcription-polymerase chain reaction (RT-PCR) was conducted to quantify the miR-512-3p expression in breast cancer and noncancerous paired specimens. Methylthiazol tetrazolium (MTT) assay, flow cytometry, and clone formation assay were used to characterize the function of miR-512-3p in breast cancer. Target prediction was performed using the TargetScan, PicTar, and miRanda software. The results were validated using RT-PCR and western blot target validation. Results:The relative expression of miR-512-3p in breast cancer specimens was significantly lower than those in normal breast specimens (P<0.05). MTT assay revealed that 48 h after transfection, miR-512-3p significantly repressed the proliferation of MD-MBA-231 cells at a suppression rate of 45.38%and at a concentration of 100 nmol/L. MiR-512-3p increased the percentage of early apoptotic cells in the treatment groups (9.32 ± 0.41)%compared with those in the blank controls (3.1 ± 0.54)%and in the negative controls (2.9 ± 0.39)%(P<0.05). Significant differences were found in the percentages of the G0/G1-and G2/M-phase cells after miR-512-3p transfection compared with those in the controls (P<0.05). In the cloning assay, clone formation was inhibited in the miR-512-3p-transfected groups compared with those in the control groups. RT-PCR and western blot results indicate that miR-512-3p significantly inhibited the c-FLIP mRNA and protein expression. Conclusion:MiR-512-3p expression is relatively decreased in breast cancer specimens compared with those in the normal samples. The negative effect of miR-512-3p on cell proliferation and clone formation and its positive effect on early apoptosis through c-FLIP targeting suggest that miR-512-3p acts as a tumor suppressor gene in breast cancer. Therefore, miR-512-3p may be a new target for the diagnosis and treatment of breast cancer in the future.

Objective To evaluate the effectiveness of transitional care model on life quality of patients with Alzheimer's disease in China.Methods The literatures of randomized controlled trials (RCT) of transitional care of patients with Alzheimer's disease in mainland China published in Chinese or English were enrolled in this review.RevMan5.1 software was used to analyze the data.Results Twelve articles met the inclusion criteria.The scores of mini-mental state examination (MMSE),health state survey questionnaire (SF-36) of the intervention group was higher than the control group,which had a significant difference.While no significant difference was found in the scores of activities of daily living (ADL) between the intervention group and the control group.Conclusions Transitional care can improve cognitive function and quality of life of patients with Alzheimer's disease in China.