There exist a number of medical psychology teaching gaps in the present Chinese medicine institutions including the course nature,teaching content,course construction and what is in line with the requirement of examination outline for professional doctors. By changing medical psychology into the required course,increasing class time,fractionizing teaching content and carrying out case teaching and practice teaching can enhance Chinese medicine graduates’ability to understand and apply the principle of modern psychology and technology.

The construction of psychology specialty in medical universities is adapted to the needs of social development and to meet people's growing need for mental health. However,there are some problems in the construction of psychology specialty,such as the difficulty to unify teaching mode,the curriculum dilemma,awkwardness of graduates'employment,which greatly affected the psychology specialty in medical universities. To resolve this dilemma,we propose a clear specialty goals,the establishment of medical colleges for psychology professionals as well as the alternative policy in the present state.

Objective To investigate the effect of thiopental on lipopolysaccharide(LPS)-induced activation of nuclear factor kappa B(NF-κB) under different concentration or after different time in rat glioma cells in vitro.Methods The rat C 6 glioma cells were treated with,in the presence or Absence of various concentrations(10-3 mol/L,10-4 mol/L and 10-5 mol/L)of thiopental for 1 h.30 min before harvest,the cells were exposed to LPS(10 ng/ml).The other C 6 cells were treated with 10-5 mol/L thiopental with different time(0.5 h,1 h,2 h,4 h).30 min before harvest,the cells were exposed to LPS(10 ng/ml).Nuclear extracts from C 6 cells were harvested using standard procedures,and the LPS-induced NF-κB expression was determined with electrophoretic mobility shift assays(EMSA) and measured with densitometry(Bio-Rad).Results The width of the bands of NF-κB under thiopental with different concentrations were similar,but they were narrower than that of the control(treated only with LPS),the density was less than that of the control(P<0.05).The width of the bands of NF-κB under thiopental with different time decreased with the time(P<0.05),and they were much narrower and shallower than that of the control(P<0.05),and the density was significantly less than that of the control(P<0.05).Conclusion Thiopental suppressed the activation of transcription factor NF-κB,and the inhibition was concentration-independent but time-dependent.

ObjectiveTo investigate the feasibility,safety and efficacy of tracheal extubation with directly connected negative pressure suction. Methods86 patients with Ⅰ or Ⅱ degree of ASA,aged 18～65 years old,without history of respiratory disease,difficult airway and ventilation problems were involved in this study.All patients undergoing endotracheal general anesthesia were randomly divided into group A and B with 43 cases in each group.Group A used negative pressure directly connected to external side of the endotracheal tube before extubation.Group B used suction tube putting into the endotracheal tube before extubation.MAP,HR,ECG,SpO2,the condition of cough and expectoration and complicatons after extubation were recorded. ResultsIn group A the MAP,HR slightly increased,SpO2 slightly decreased,ECG showed no significant change,but the differences were not significant.In group B,MAP,HR,SpO2 showed significant difference compared with those before extubation and the corresponding period of group A. ConclusionsThe method that connecting negative pressure suction with endotracheal tube can effectively reduce the cardiovascular response during extubation,It also can avoid hypoxia,reduce the tracheobronchial injury and pulmonary complications.It's a practical good method in tracheal extubation.

Objective To compare the abnormal influence of Ureaplasma urealyticum(UU) and human papillomavirus(HPV) infection on early pregnant women,and to investigate the correlation between reproductive tract infection with spontaneous abortion in early pregnant women.Methods A total of 460 pregnant women were included in the observation subjects,including 230 cases of spontaneous abortion (observation group) and other 230 normal pregnant women of delivery as the control group.The cervical swab samples were collected from the research subjects of the two groups and performed the UU and different types of HPV detections by using corresponding PCR reaction reagents.Results The UU total infection rate among 460 pregnant women was 17.83%(82/460),which of the observation group was 24.8%(57/230) and which the control group was only 10.9%(25/230),the difference between the two groups was statistically significant(P<0.01).The HPV total infection rate was 20.2%(93/460),which of the observation group was 29.1%(67/230) and which of the control group was only 11.3%(26/230),the difference was statistically significant.Among them,the single subtype HPV infection proportion was 14.1%(65/460),the multiple subtype HPV mixed infection proportion was 6.1%(28/460),while the high risk type HPV infection rate was 18.3%(84/460) and the low risk type HPV infection rate was 8.91%(41/460).The infection rate of UU combined HPV was 2.6%(12/460),in which the observation had 11 case and control group had 1 case,the difference was statistically significant (P<0.01).Conclusion Infection of UU and HPV in early pregnant women may lead to spontaneous abortion.But its concrete mechanism and the role of different types of HPV need to be further studied.

Objective:To establish the HPLC fingerprints of Atractylodes lancea in northwest of Hubei province to provide the basis for the quality control and variety identification of Atractylodes lancea. Methods:The fingerprints were detected by HPLC. The separa-tion was achieved on a Diamonsil 2# C18(250 mm ×4.6 mm,5 μm)chromatographic column at 30℃ using acetonitrile-water (gradient elution) as the mobile phase at a flow rate of 1. 0 ml·min-1 . The detection wavelength was 340nm. Results: There were 13 common peaks in the HPLC fingerprints of 14 batches of Atractylodes lancea with promising separation. According to the results of clustering a-nalysis,Atractylodes lancea in northwest of Hubei province was clustered into two categories. Conclusion:A simple, feasible and relia-ble method is provided for the quality control of Atractylodes lancea.

Objective: To optimize the extraction conditions of semi-bionic extraction for Gardenia jasminoides Ellis. Methods:The best extraction conditions of the semi-bionic extraction for Gardenia jasminoides Ellis was screened by uniform design with yield of geniposide, total iridoid glycosides and dry extract as the indices. Results:The optimal conditions were as follows:the pH value of wa-ter for the lst, 2nd and 3rd decoction was 2. 0, 6. 5 and 9. 0, respectively, and the extraction time was 2. 0, 1. 0 and 1. 0h according-ly. Conclusion:The optimized extraction conditions by uniform design is scientific and reasonable.

Animals ; Animals, Newborn ; Cells, Cultured ; Corticotropin-Releasing Hormone ; pharmacology ; Interleukin-6 ; metabolism ; Microglia ; cytology ; drug effects ; metabolism ; Nitric Oxide ; metabolism ; Rats ; Rats, Sprague-Dawley ; Tumor Necrosis Factor-alpha ; metabolism

With the deepening of research in recent years, tumor metabolic reprogramming has gradually become the focus of research, and targeting tumor cell metabolism has also become a new means of tumor therapy. The metabolic process affects almost all the physiological processes of the organism, and lipid metabolism is an important part of the metabolic process. Studies have shown that changes in lipid uptake, storage and fatty acid synthesis and decomposition have occurred in a variety of tumors. Abnormal lipid metabolism will promote the rapid proliferation of tumors. Abnormal expression of a variety of key metabolic enzymes in the process of lipid metabolism is the key to tumor progression. The purpose of this paper is to explain the metabolic regulation of lipid metabolism and related metabolic enzymes in hematological tumors, and to provide ideas for the treatment of hematological tumors.

Signal transducer and activator of transcription 3 (STAT3) is an important regulatory factor of cell proliferation and metastasis, involved in the occurrence and development of a variety of malignant tumors, and it is one of the hot spots in the research of targeted anti-tumor drugs. Our group screened a novel benzobis (imidazole) structure small molecule compound LZJ541 through the screening model of Janus kinase (JAK)/STAT3 pathway inhibitors, which has definite STAT3 inhibitory activity. We examined the effect of LZJ541 on the proliferation of HepG2 and PC-3 cells by MTT assay in vitro, detected the effect of LZJ541 on the expression of STAT3-related proteins in HepG2 cells by Western blot, and measured the effect of LZJ541 on the apoptosis and cell cycle arrest of HepG2 cells via flow cytometry. The results indicated that LZJ541 significantly inhibited the activation of STAT3 signaling pathway and restrained the proliferation of HepG2 cells. Its half maximal inhibitory concentration (IC₅₀) was 13.8 μmol·L^-1, which was much lower than that of PC-3 cells (with low STAT3 expression, IC₅₀: 41.99 μmol·L^-1), LZJ541 can also inhibit the phosphorylation of STAT3 in HepG2 cells, thereby inducing apoptosis and cycle arrest and then exerting anti-tumor effects. In conclusion, LZJ541 has a certain anti-tumor effect in vitro, which provides an experimental basis for the development of new STAT3-targeted anti-tumor drugs around this kind of compounds.