Adenoma ; metabolism ; pathology ; surgery ; Aged ; Diagnosis, Differential ; Endodermal Sinus Tumor ; pathology ; Female ; Humans ; Immunohistochemistry ; Neprilysin ; metabolism ; Ovarian Neoplasms ; metabolism ; pathology ; surgery ; Sex Cord-Gonadal Stromal Tumors ; pathology ; Stromal Cells ; metabolism ; pathology ; Vimentin ; metabolism

Platelet-rich plasma (PRP) is a concentrated source of autologous platelets after centrifugation, being used widely in plastic surgery and oral-maxillofacial surgery. Recent studies have showed that PRP can improve bone repair, vasculogenesis, and soft tissue repair. Composite of PRP and stem cells is applied in the field of orthopedics and tissue engineering. As a matter of fact, PRP has become a hot research topic. In addition, PRP plays an important role in the healing of chronic wound. This paper briefly introduces the main components of PRP and its clinical application, the mechanism for bone healing and soft tissue repair, as well as the latest research achievement and discussion in the shortcoming of PRP in clinical application.

Aim To prepare the monoclonal antibodies (mAbs) against human CD28 and to study its biological feature. Methods The hybridoma cell lines were obtained by fusing spleen cells of Blab/c mice that had been immunized with murine lymphoma cells transfected with full-length huaman CD28 cDNA to myeloma cells Sp2/0. Ascites were induced to produce the mAbs. The specificity and affinity of the mAb 18G8 was verified by CD28 competitive inhibitory test and FACS. Reactivities of mAb 18G8 to PBTC, U266, 8226, Jurkat and Daudi cell were studied by indirect immunofluorescence staining. mAb 18G8-inducing proliferation of peripheral blood T cells (PBTCs) was determined by [3H]thymidine incorporation test. Results Five hybridoma cell lines were obtained. mAb 18G8 secreted by one of the them, belong to mouse IgG2a. It recognized a epitope different from which recognized by the standard mAb(clone CD28.2). The Reactivitrates of the mAb 18G8 to PBTC, U266, 8266, Jurkat and Daudi cells were 70.2％ , 99.3％ , 98.6％ , 76.4％ and 1.9％ , respectively, similar with CD28.2. It was indicated that different antigen epitopes expressed on all above cells. mAb 18G8 could promote the PBTC proliferation in vitro(SI=7). It was indicated that The substitution of mAb 18G8 for B7-1 molecule could also mediate the costimulatory signals. Conclusion 18G8 is a specific and functional anti-CD28 mAb it may be of significant value in basic studies and clinical application.

Objective To evaluate a new enzyme-linked immunospot assay (TSPOT-TB) for the diagnosis of latent tuberculosis infection in patients with rheumatic diseases.Methods The rapid TSPOT-TB assay was applied to detect ESAT-6 and CFP-10 specific T cells in blood samples from 126 rheumatic disease patients.A PPD skin test was performed on all patients simultaneously.Results The positive rate of TSPOT assay was 23.8% and that of PPD skin test was 34.9%.The overall agreement between the 2 tests was 71.4%.Among PPD (-) patients (n=82),11 were TSPOT (+) ( 13.4% ).Among PPD (+) patients (n=44),25 were TSPOT(-) ( 56.8% ).The patients who got BCG vaccination showed a significantly higher rate of positive results of PPD skin test than those who did not(41% vs 19%,P＜0.05).While in TSPOT assay,the BCG vaccination did not show any influence on TSPOT results (22% vs 27%,P＞0.05).Conclusion BCG vaccina-tion affects the results of PPD test in patients with rheumatic diseases,but has no influence on TSPOT results.The infection rate of latent tuberculosis in patients with rheumatic diseases in our study is 23.8% detected by TSPOT.

Objective To compare the efficacy of the conventional PPD skin test and a new enzymelinked immunospot assay(TSPOT-TB)for diagnosing latent tuberculosis infection(LTBI)in patients with rheumatic diseases.Methods Two hundred and sixty rheumatic patients were enrolled,and all were screened for LTBI based on clinical history,chest X-ray,PPD skin test or TSPOT.Results The positive rate of TSPOT assay was 24.1%and that of PPD skin test was 39.4%.The overall concordance rate between the 2tests was 61.0%.Among PPD negative patients (n=149).29 were TSPOT(+)(19.5%).Among PPD(+)patients(n=98),69 were TSPOT(-)(70.0%).The patients who got BCG vaccination or had history of tuberculosis infection showed a significantly higher rate of positive result of PPD skin test than those who did not (P<0.05 or P<0.01).While in TSPOT assay,the BCG vaccination or history of tuberculosis infection did not show influence on TSPOT results(P>0.05).Of the 127 patients who received biological agents after screening for LTBI,9 patients were pretreated with isoniazide.Twenty-seven patients stopped biological agent treatment because of the positive results of PPD or TSPOT.Twenty three patients who had positive PPD but negative TSPOT results received biological agent treatment without isoniazide,and none of them developed active tubereulosis after 6 to 18 months of follow-up.Conclusion BCG vaccination affects the result of PPD test in rheumatic patients,but has no influence on TSPOT results.The infection rate of latent tuberculosis of rheumatic patients in our research is 23.8%detected by TSPOT.

Objective To analyse the femoral head coverage rate of CT in evaluation of Salter osteotomy for developmental dislocation of hip(DDH) in children. Methods Thirty-eight patients with unilateral DDH for Salter osteotomy were enrolled,and X ray photography of pelvis and spiral CT scanning of femoral articulation were performed one week before and 6 months after Salter osteotomy.The femoral head coverage rate of X ray and that of CT were calculated and compared.Another 38 children with normal femoral articulation were served as controls. Results The femoral head coverage rate of X ray was significantly higher than that of CT in patients with DDH(P

The main type of four root canals in maxillary second molar is two-mesiobuccal. Incidence rate of maxillary second molar with two palatal root canals is especially rare. A case of maxillary second molar with two palatal root canals was reported in this article.
Dental Pulp Cavity ; Humans ; Maxilla ; Molar ; Tooth Root

Objective To systematically evaluate the efficacy and safety of aminoglycoside antibiotics in the treatment of ocular infection through subconjunctival injection.Methods PubMed,EmBase,Cochrane library,Wanfang and CNKI databases were searched from inception until March 2017 to investigate clinical trials about subconjunctival injection of aminoglycoside antimicrobials for the treatment of eye infection.Systematic evaluation was used to analyze the main indexes such as the cure rate of eye infection,the drug concentration in aqueous humor,the occurrence of eye pain after injection and the incidence of conjunctival edema.Results A total of 18 randomized controlled trials were enrolled in our study.The results showed that the cure rate of eye infection through subconjunctival injection of aminoglycoside antibiotics was excellent and an adequate aqueous humor concentration could be attained.No systemic toxicity was found,but the incidences of eye pain (39.62％) and conjunctival edema (72.58％) were high.Conclusion Subconjunctival injection of aminoglycoside antibiotics was effective.Considering the low quality of included studies,the safety of subconjunctival injection of aminoglycoside antibiotics needs to be further verified.

Objective To develop a method for concentration determination of voriconazole in human plasma in order to apply to clinics.Methods HPLC-MS/MS method was adopted.Using d3-voriconazole as internal standard,the determination was performed on Agilent Eclipse Plus C18 column with mobile phase consisted of acetonitrile-water (containing 0.01 mol · L-1 ammonium formate) 55∶45 at flow rate of 0.3 mL · min-1 and column temperature of 40 ℃.The electrospray ion source,positive ionizing pattern and multiple reaction monitoring were used;the mass transition ion-pairs of voriconazole and internal standard were m/z 350.2→m/z 281.1 and m/z 353.2→m/z 284.1.Results The linear standard concentration range of voriconazole was 50-1.0 ×104 ng · mL-1(r =0.9997),and the lower limit of quantitation was 50 ng · mL-1.Precision (RSD) of inter-day and intra-day were lower than 5％.Method recovery was higher than 95％,and extraction recovery was higher than 85％.The steady state trough voriconazole plasma concentrations of 17 patients with hematologic malignancy were 0.38-8.10 μg · mL-1 determined by this method,of which 3 patients were outside of the recommended range.Conclusion The established assay method in this study is rapid,accurate and sensitive,and can be applied for the therapeutic drug monitoring of voriconazole.

Objective:To research the clinical effects of ulinastatin in the treatment of sepsis induced acute renal injury and its possible mechanisms.Methods:114 cases of patients with sepsis induced acute kidney injury from 2014.02 ～ 2016.08 were selected and randomly divided into the control group (n=57) and experimental group (n=57) according to the draw method,the control group was given conventional treatment,while the experimental group was treated by ulinastatin based on the control group,the urine urinary injury molecule-1 (KIM-1),atrialnatriuretic peptide (ANP),cyscatin-c (CYS-C),interleukin l,6 (IL-1,IL-6),c-reactive protein (CRP),tumor necrosis factor-α(TNF-α),nitric oxide (NO),endothelin 1 (ET-1),immunoglobulin A,G,M (IgA,IgG,IgM) levels,APACHE-Ⅱ score were compared between two groups before and after the treatment.Results:After treatmented,the urine of KIM-1,ANP,serum of CYS-C,IL-l,IL-6,CRP,TNF-α,ET-1 levels and APACHE-Ⅱ score of experimental group were significantly lower than those of the control group (P＜0.05).The serum NO,IgA,IgG,IgM levels of experimental group were significantly higher than those of the control group (P＜0.05).Conclusion:Ulinastatin could significantly relieve sepsis induced acute renal injury,which might be related to the inhibition of inflammatory response,improvement of the renal blood flow and immune function.