Objective To investigate the potential role of FoxP3-expressing CD4+CD25+ regulatory T cells in children with asthma.Met-hods Intracellular 3-colour flow cytometry and real time PCR were used to assess the expression of FoxP3 in peripheral blood mononuclear cell(PBMC)of asthma,repectively.Results There were similar frequencies of CD4+ CD25+ T cells in peripheral blood of asthma patients and healthy subjects.However,the percentage of CD4+CD25high and CD4+CD25+FoxP3+ in asthma group were lower than those in healthy subjects(Pa

Objective To study the clinical feature and treatment of lung benign metastasizing leiomyoma. Methods A retrospective analysis was performed on 2 cases of lung benign metastasizing leiomyoma. Results Two cases were asymptomatic who also had previously undergone hysterectomy, but imageology revealed multiple pulmonary nodules bilaterally of various sizes. And one of them had a pelvic mass. Conclusion Lung benign metastasizing leiomyoma is a rare disease. Hysterectomy, bilateral salpingo-oophorectomy and hormonal therapy may be helpful to improve the prognosis.

Objective To study the CT characteristics of rare renal malignant tumors.Methods 7 cases with rare renal malignant tumors confirmed by operation and pathology were reviewed and their CT manifestations were analyzed retrospectively.In clinical, all cases had abdomen pain,6 cases had macroscopic haematuria,and 1 case had asymptomatic by chance.Results Of 7 cases,3 ca-ses were sarcomatoid carcinoma,1 case was carcinosarcoma,1 case was malignant mesenchymal tumor,1 case was lymphoma,and 1 case was neuroendocrine carcinoma.The diameter was about 4.0-12.3 cm.1 case had uniform density,6 cases uneven,and the boundary was not clear.Conclusion The rare renal malignant tumors are misdiagnosed easily.Combined clinical data with imaging performance are helpful to make comprehensive analysis,and to improve accuracy rate for diagnosis,definite diagnosis still depends on pathological examination.

Objective:To investigate and analyze the situation of professional identity of new undergraduates in medical image profession, and guide and arouse the learning initiative, provide education guidance with more pertinence for the professional plan of undergraduate, and enhance employment competence of graduates, and improve the social status of this profession.Methods:The questionnaire survey, comparison of results and trend analysis of relative question for new undergraduates of image specialty were collected and analyzed, and then the general situation of professional identity was obtained.Results: The professional identity of new undergraduates were below the average level, and their cognition for employment situation were not clear, and their evaluations for this profession were lower. Besides, the situations of male were lower than that of female.Conclusion: Face to the trend of fast development of image specialty and shortage of image professionals, the analysis of professional identity for new undergraduates is worthy because it can improve teaching quality, enhance quality for image talent and contribute to establish thorough employment guidance plan for colleges and universities.

Objective To characterize the CT features of peripheral small lung cancers of fast growthing and to correlate such features with pathological findings.Methods 12 peripheral small lung cancers(

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Adjuvants, Immunologic ; chemistry ; pharmacology ; Anti-Inflammatory Agents, Non-Steroidal ; chemistry ; pharmacology ; Clerodendrum ; chemistry ; Drugs, Chinese Herbal ; chemistry ; pharmacology ; Humans ; Phytotherapy ; Urinary Tract Infections ; drug therapy

Objective:To improve the quality standard for Kangshiming mixtures. Methods:The identification of Angelicae Sinen-sis Radix, Astragali Radix and Phellodendri Chinensis Cortex was carried out by TLC. The contents of puerarin and paeoniflorin in the preparations were determined by HPLC. Results:The spots displayed in TLC were clear without interference from the negative control. The linear range for puerarin was 1. 64-49. 20μg·ml-1(r=0. 999 9) and 4. 22-63. 30μg·ml-1(r=0. 999 6) for paeoniflorin. The average recovery was 99. 63%(RSD=2. 14%,n=9) and 99. 05%(RSD=2. 70%,n=9) for puerarin and paeoniflorin, respectively. Conclusion:The method is accurate, reliable and specific, and can be used in the quality control of Kangshiming mixtures.

ObjectiveTo investigate the role of heme oxygenase-1 (HO-1 ) in process of sevoflurane preconditioning attenuating hypoxia-reoxygenation (H/R) injury in cultured neonatal rat cardiomyocytes.Methods Primary cultured neonatal rat cardiomyocytes were randomly divided into four groups ( n ＝ 25 each):control group (group C),group H/R,sevoflurane preconditioning group(group S + H/R),and HO-1 inhibitor zinc protoporphyria (ZnPP) and sevoflurane preconditioning group(group ZnPP + S + H/R).In group H/R,the cardiomyocytes were exposed to 2 hours of hypoxia,followed by 1 hour of reoxygenation.Group S+ H/R received 2.5％ sevoflurane preconditioning for 20 minutes followed by 10 minutes of wash-out before H/R.ZnPP was added to the culture medium with final concentrations of 3 μMol/L 1 hour before sevoflurane preconditioning and H/R in group ZnPP +S + H/R.HO- 1 expression,apoptosis rate,concentration of free calcium ( [ Ca2 + ] i),mitochondrial membrane permeability transition pore (PTP),cytoohrome C ( Cyto C) expression and activities of lactate dehydrogenase (LDH) and creatine kinase (CK) in culture supernatant were detected after reoxygenation.ResultsCompared with group C,the expression of HO-1 and cytoplasmic Cyto C of cardiomyocytes were up-regulated,mitochondrial Cyto C was down-regulated,while the [Ca2+ ]i,opening degree of PIP,apoptosis rate and activities of LDH and CK in culture supernatant were increased in group H/R.Compared with group H/R,the expression of HO-1 and mitochondrial Cyto C of cardiomyocytes were up-regulated,cytoplasmic Cyto C was down-regulated,while the [Ca2+ ] i,opening degree of PTP,apoptosis rate and activities of LDH and CK in culture supernatant were decreased in group S + H/R.Compared with group S + H/R,the expression of HO-1 and mitochondrial Cyto C of cardiomyocytes were down-regulated,cytoplasmic Cyto C was up-regulated,while the [Ca2+ ]i,opening degree of PTP,apoptosis rate and activities of LDH and CK in culture supematant were increased in group ZnPP + S + H/R.ConclusionThe up-regulation of HO-1 is involved in the process of sevoflurane preconditioning attenuating hypoxia-reoxygenation injury in cultured neonatal rat cardiomyocytes.

Objective To explore the protective effects of polysaccharide from Panax japonicus on free fatty acid in different parts of hepatic cell injury. Methods Polysaccharide of Panax japonicus was prepared through different concentrations of ethanol precipitation and was named as 30%polysaccharide component (pc), 60% pc and 90% pc. Palmitic acid (PA) was used to induce a cellular model of steatosis in HepG2 cells in order to screen the intervention viability of polysaccharide of Panax japonicus. MTT method was used to detect cell viability, and Oil Red O staining was used to demonstrate steatosis. Total RNA was extracted to detect the expression level of the relevant genes. Results MTT results showed that the 30% pc significantly increased cell viability compared with the model group;Oil Red O staining showed that the number of intracellular lipid droplets was significantly reduced in the 30% pc compared with the model group;RT-PCR results showed that expressions of the endoplasmic reticulum stress-related gene glucoese-regulated protein, CCAAT enhancer binding protein homologous protein and TNF-αwere significantly lower in the 30% pc compared with the model group, and there was no significant difference compared with normal control group. Conclusion The 30%ethanol precipitation fraction of polysaccharide from Panax japonicus significantly reduced PA-induced steatosis in HepG2 cells. Its mechanism was possibly realized through intervention in endoplasmic reticulum stress-related response.