Acute Disease ; Adult ; Female ; Humans ; Male ; Organometallic Compounds ; poisoning ; Poisoning ; therapy

As a subset of Inhibitory T cells,CD4+ CD25+ Foxp3+ regulatory T (Treg) cells could strongly inhibit the activation,proliferation and function of effector T lymphocytes cells.Treg cells play an important role in downregulate autoimmune and mediating transplantation tolerance.Treg cells concerntrations could be markedly increased expression in tumor issues and peripheral blood of lung cancer.The clinical staging of lung cancer and lung cancer pathological types.Treg cells are important for lung cancer diagnosis and treatment.

OBJECTIVE:To provide reference for the development of online drugstores in China based on mobile port. METH-ODS:The development of online drugstores in China based on mobile port was analyzed by SWOT(strengths,weaknesses,oppor-tunities,threats)analysis to put forward suggestions. RESULTS & CONCLUSIONS:Online drugstores based mobile port included vertical management in which pharmaceutical E-commerce manager directly participate,non-vertical management which mainly pro-vide information inquiry and price comparison. It has advantage in breaking through time and space obstacle,optimizing informa-tion display,integrating consumer’s information accurately,improving human efficiency,etc. They are weak in timeliness,busi-ness scope and sale function,the quality of pharmaceutical care,user activity,etc. They have the opportunities as annual increase of mobile E-commerce market scale and huge market development potential;they also face various threats,such as the inconformi-ty between main users of mobile port and main purchasers,consumer low-trust,health-care polity barrier breakthrough failure,the lack of legal regulations for medical and pharmaceutical software,etc. It is suggested that companies operate further accurately and the government speed up the formulation and implementation of laws and regulations.

Traditional Chinese medicineZhizi is the dry ripe fruit of Gardenia jasminoides Ellis, which belongs to the family of Rubiaceae. The main effective constituents of gardenia include iridoid, gardenia yellow pigment and organic acid. Modern pharmaco-logical studies showed that the main pharmacological effects of gardenia included anti-inflammatory, antipyretic and analgesic effects, liver protection, cholagogue, antilipidemic effect, antithrombus, nerve protection and anti-Alzheimer' s disease. In this paper, the ef-fective constituents and pharmacological effects of Gardenia jasminoides Ellis were reviewed to provide available information for further research.

AIM:To discuss the causes and therapeutic measures of Descemet's membrane detachment during phacoemulsification ( Phaco) .
METHODS:A retrospective analysis of 997 patients who had operations of Phaco and intraocular lens ( IOL ) implantation at the eastern part of Yangzhou No. 1 People's Hospital during 2007-2012. The treatment and prognosis of 21 patients who had the complication of Descemet's membrane detachment during the surgery were observed.
RESULTS: The vision acuity of all the 21 patients had improved to different extent after therapy, no one had the decompensation of corneal endothelium.
CONCLUSION:Early detection and treatment is decisive for the vision acuity of the patients who had the Descemet's membrane detachment.

Child ; Clinical Laboratory Techniques ; Humans ; Microbiological Techniques ; Viral Load ; Virus Cultivation ; Virus Diseases ; diagnosis

BACKGROUND:The studies about biomechanics of total knee arthroplasty were more, but the research on finite element analysis of the effect of total knee arthroplasty on proximal tibial reconstruction was not much.
OBJECTIVE:To study the effect of total knee arthroplasty on proximal tibial reconstruction using finite element analysis.
METHODS:We designed knee models of two materials:one was functional y graded material models of col agen and titanium composite materials;another was titanium al oy material model. We created two prosthesis material tibia-knee prosthesis model, and analyzed bone density distribution of proximal tibia, the maximum stress value of the medial tibia and the lateral tibia, and the maximum stress value of medial tibia and prosthesis junction, as wel as lateral tibia and prosthesis junction after knee arthroplasty.
RESULTS AND CONCLUSION:(1) Bone density of proximal tibia after total knee arthroplasty:The bone density of the knee prosthesis handle with medial and lateral tibia at the junction of tibial plateau was decreased than that of the initial state. Stress shielding effect was lower in col agen and titanium composite materials of functional y graded material models than that of titanium al oy material model. The bone density of shank end of the tibial prosthesis was obviously increased compared with the medial and lateral tibia, showing stress concentration. (2) The maximum stress value of the medial and the lateral tibia:The maximum stress value of the medial and the lateral tibia in col agen and titanium composite models was lower than that of titanium al oy material model. The maximum stress values of the lateral tibia of col agen and titanium composite models and titanium al oy material model were lower than that of medial tibia. (3) The maximum stress of medial tibia, lateral tibia and prosthesis junction:The stress distribution of col agen and titanium composite models was relatively uniform. The stress distribution of titanium al oy material model showed fluctuation. Stress concentration appeared in pal ets and prosthesis junction and the shank end of the prosthesis. The maximum stress values of lateral tibia and prosthesis junction of col agen and titanium composite models and titanium al oy material model were lower than that of medial tibia and prosthesis junction. (4) These findings showed that knee prosthesis has a certain stress shielding effect on tibia after total knee arthroplasty. The stress shielding effect of col agen and titanium composite material prosthesis was lower than titanium al oy material prosthesis, and could improve tibial incurred stress.

Objective: To investigate the inhibition of luteoloside on the proliferation of human esophageal carcinoma cell line Eca109 and its mechanism. Methods: MTT assay was used for detecting the influence of luteoloside at different concentration on the proliferation of Eca109. The morphological changes of cells were observed under inverted microscope. The cell cycle and apoptosis were detected by flow cytometry. RT-PCR was used to detect the mRNA expression of cyclin D1, survivin, and c-myc genes. Results: The results of MTT assay showed different doses (80, 120, 160, 200, and 240 μmol/L) of luteoloside could inhibit the cell proliferation of Eca109 cells in a dose-response manner. Luteoloside could also change the morphological characteristics of cells, reduce the cell size, and separate from peripheral cells. Treated with 240 μmol/L luteoloside, the cells were sprouting and some of them developed multiple pseudopodia-like protrusions. Treated with luteoloside (160 and 240 μmol/L), the cell cycle of Eca109 cells was blocked in G2/M phase and apoptosis was induced (P < 0.05). Compared with the control group, the gene expression of cyclin D1, survivin, and c-myc was decreased after treated with 240 μmol/L luteoloside for 48 h (P < 0.05). Conclusion: Luteoloside could significantly inhibit the proliferation of Eca109 cells by changing the cell cycle and inducing the apoptosis. Moreover, it could decrease the mRNA expression of relative genes.

Objective To evaluate the current knowledge and practice of evidence-based nursing for preventing ventilator-associated pneumonia (VAP) and its influencing factors in ICU nurses. Methods Selfadministered structured questionnaire was used in 101 ICU nurses working in one grade three A hospital of Jiangsu province by convenient sampling method. The results underwent statistical description and variance analysis. Results The mean score of knowledge about evidence-based nursing to prevent VAP was (4.75±1.42) (total score=10),the mean score of practice was (3.32±0.32) (total score=4). The influencing factors of knowledge in ICU nurses were different divisions and ICU working years. Different ICU working years resulted in different behavior of nurses. Most nurses considered that nursing work was too busy due to serious condition of patients, which was the principal interfering factor in practice of evidence-based nursing for preventing VAP. Conclusions Further expertise of ventilator-associated pneumonia training and evidence-based nursing education are urgently required. The best-practice guidelines for preventing VAP are more often consummated. Whilst encouraging the reasonable personnel disposition and the essential facilities are priority. Effective fulfillmere in evidence-based practice to prevent VAP may be achieved by broader adjustment of the medical system and the medical cost structure, so that evidence-based nursing for preventing VAP can be put into effect.

Objective To compare the differences of self-imposed delay of gratification and the characteristics of delay strategy between aggressive children and normal children, in order to provide evidence-based basis for aggressive children's education and behavior modification. Methods Using the classical self-imposed delay of gratification task to compare the ability of delay of gratification between 24 aggressive children and 24 class-, age-, gender-matched normal controls. Recording children' s delay behavior coding to compare their differences of delay strategy by using the time sampling observation method. The differences of purpose of delay of gratification was analyzed with χ2 test. The differences of their delay strategy was analyzed with the independent sample t -test. Results A significant difference was found between two groups on delay of gratification(χ2 = 13. 50, P ＜0.01). The controls showed more purpose during the waiting process than the aggressive children whose waiting was mostly without purpose or they simply gave up. The problem-solving strategy was hightlighted in the use of delay strategy for both the aggressive children(22. 38 ±11. 29) and the controls( 28. 21 ± 3. 05). The control group children used more problem-solving strategy(28.21 ±3.05) and self-control strategy(0. 71 ± 1.08) during the waiting task. Their level of delay strategy was higher than the aggressive groups ( (22. 38 ± 11. 29), (0. 13 ± 0. 45) ) (P ＜ 0. 05). Conclusion The aggressive children are less capable of self-imposed delay of gratification,the purpose of behavior is weaker and the level of delay strategy is lower than normal children.