1.Staging syndrome differentiation and treatment of radiation pneumonitis
China Journal of Traditional Chinese Medicine and Pharmacy 2005;0(08):-
By staging radiation pneumonitis’ syndrome and discussing the pathogenesis, the course of radiation pneumonitis is divided into acute phase, lag phase and anaphase. Acute phase is treated with clearing heat and resolving phlegm, cooling ying and nourishing yin; lag phase is treated with supplementing qi and nourishing yin, promoting blood circulation and resolving phlegm; anaphase is treated with nourishing lung and strengthening kidneys, resolving stasis and dredging collaterals.
2.Detection of human parvovirus B19 nonstrutural protein DNA by nested-polymerase chain reaction in gravida serum and pregnant tissues.
Ting, SHEN ; Yongmei, HUANG ; Fuyuan, QIAO ; Zengqing, LI ; Haiyi, LIU
Journal of Huazhong University of Science and Technology (Medical Sciences) 2006;26(1):123-6
A new nested-polymerase chain reaction (nested-PCR) assay was developed to detect human parvovirus B19 DNA corresponding to the nonstructural protein in clinical specimens in a routine diagnostic laboratory. The sensitivity of this highly specific assay was up to 0.005 fg of B19 DNA. Parvovirus B19 was identified in sera of 20 pregnant women with abnormal pregnant outcome. Among these 20 cases, intrauterine parvovirus infection did exist in 7 pregnant women because parvovirus B19 DNA was detected in the pregnant tissues of them such as placenta tissues, chorionic villi, amniotic fluid, fetal spleen, liver and abdominal fluids.
DNA, Viral/*analysis
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Parvoviridae Infections/*virology
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Parvovirus B19, Human/genetics
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Parvovirus B19, Human/*isolation & purification
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Placenta/virology
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Pregnancy Complications, Infectious/diagnosis
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Pregnancy Complications, Infectious/*virology
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Viral Nonstructural Proteins/*analysis
3.Development of Computer Real Time Monitoring System for Rational Clinical Use of Drugs
Ting WANG ; Hua QIAO ; Li LIANG ; Hong ZHANG ; Ping LI
China Pharmacy 1991;0(01):-
OBJECTIVE:To promote rational clinical use of drugs by the aid of computer.METHODS:The databases for basic data of drugs,irrational use of commonly-used drugs,compatibility of agents for injection and consultation for drug use in periods of prequancy and lactation were established.Modules for inquiry,addition,revision,deletion and maintaince were designed.The computer real time monitoring system for rational clinical use of drugs was thus developed.RESULTS&CONCLUSION:This system is simple,flexible and quick,and can check the prescription and provide consultation for compatibility between drugs.It is suitable for extensive use in clinical practice.
4.Expression of novel apoptosis-related protein PDCD5 in granulosa cells of polysystic ovary syndrome
Chunling SUN ; Jie QIAO ; Zhenxing HU ; Ting ZHANG ; Yingyu CHEN
Journal of Peking University(Health Sciences) 2003;0(05):-
Objective: To detect the novel apoptosis-related protein PDCD5 expression in granulosa cells of polysystic ovary syndrome(PCOS) and normal ovary, and explore the pathogenesis of PCOS. Methods:The granulosa cells were collected from 30 cases of PCOS and normal ovary in IVF-ET. Expression of PDCD5 was detected by flow cytometry; immunofluorescence and immunohistochemistry. Cell apoptosis was detected by Propidium Iodide (PI) staining. Results: The number of hypodiploidy cells associated with apoptosis in granulosa cells of PCOS was greater than that of the normal control. PDCD5 protein expression in PCOS granulosa cells was significantly higher than that in normal ovary(P
5.Research progress of phytoestrogens-like chemical constituents in natural medicines.
Ting-Ting YUAN ; Nai-Dan ZHANG ; Yong-Jing HE ; Mei LI ; Hong-Tao XU ; Qiao-Yan ZHANG
China Journal of Chinese Materia Medica 2014;39(23):4526-4531
Phytoestrogens, which can bind with estrogen receptor and produce estrogen-like effects, are a kind of nonsteroidal compound in plant. Phytoestrogens chemically include isoflavones, coumarins, lignans and other compounds. Phytoestrogens are selective estrogen receptor modulator, and have therapeutical effects on breast cancer, prostate cancer, cardiovascular disease, menopausal symptoms, osteoporosis and other disease, however, do not produce stimulatory hyperplasia effects on uterus, mammary glands and other tissues and organs with positive estrogen receptor. Long-term exposure or excessive use of phytoestrogens maybe affects male reproductive system and hematopoietic function of fetus. Some questions need to be further studied, such as evaluation criteria on biological activity, adverse effects, and action mechanism of phytoestrogen. This review covers plant sources, chemical structure, pharmacological activity and safety of phytoestrogens. It will provide a useful reference for intensive research and rational utilization the phytoestrogens.
Animals
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Humans
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Phytoestrogens
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chemistry
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pharmacology
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Phytotherapy
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Plant Extracts
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chemistry
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pharmacology
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Plants, Medicinal
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chemistry
6.Activation of hippocampal D1 dopamine receptor inhibits glutamate-mediated depression induced by chronic unpredictable mild stress in rats.
Ting-Ting YUAN ; Hui QIAO ; Su-Ping DONG ; Shu-Cheng AN
Acta Physiologica Sinica 2011;63(4):333-341
The present study was to investigate the role of dopamine D1 receptors and its relationship with glutamate, N-methyl-D-aspartic acid (NMDA) receptor and α-amino-3-hydroxy-5-methylisoxazole-4-propionic acid (AMPA) receptor in depression induced by chronic unpredictable mild stress (CUMS). CUMS-induced depression model was established in Sprague-Dawley rats, and intrahippocampal microinjections of D1 dopamine receptor agonist SKF38393, non-competitive NMDA receptor antagonist MK-801 and AMPA receptor antagonist NBQX were respectively adopted by rat brain stereotaxic coordinates. The behavioral observations were conducted by measurement of weight changes, sucrose preference test, open-field test and tail suspension test. The concentration of glutamic acid and the expression of its receptors' subunits were detected by HPLC and Western blot, respectively. The results showed that, compared with control group, CUMS rats showed depression-like behavioral changes, higher concentration of glutamic acid, lower expressions of NMDA receptor (NR1) and AMPA receptor (GluR2/3) in hippocampus. Pretreatment with injection of SKF38393 could rescue such depression effect of CUMS, decrease the concentration of glutamic acid, and increase the expressions of NMDA receptor (NR1), AMPA receptor (GluR2/3) in hippocampus. Pretreatment with MK-801 could enhance the antidepressant effect of SKF38393, while NBQX weakened. These results suggest that agonists of D1 dopamine receptor could reduce the concentration of glutamic acid in hippocampus, and its antidepressant effect may be mediated by AMPA receptor partially.
2,3,4,5-Tetrahydro-7,8-dihydroxy-1-phenyl-1H-3-benzazepine
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pharmacology
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Animals
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Depression
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etiology
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physiopathology
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Dizocilpine Maleate
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pharmacology
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Excitatory Amino Acid Antagonists
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Glutamates
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metabolism
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Hippocampus
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metabolism
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Male
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Rats
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Rats, Sprague-Dawley
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Receptors, AMPA
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metabolism
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Receptors, Dopamine D1
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agonists
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physiology
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Stress, Physiological
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physiology
7.Effect of blue light on proliferation of human retinal pigment epithelial cells
Hong-Na, ZHU ; Ying, QIAO ; An-Le, SU ; Ting, ZHANG ; Zhong-Yang, SUN ; Hou-Cheng, LIANG
International Eye Science 2017;17(8):1419-1422
AIM: To investigate the influence and mechanism of blue light on the proliferation of human retinal pigment epithelial cells.METHODS: Cells were divided into two groups,including blue light group and control group.The 35W white light lamp with blue filter was used to establish damaged RPE cell model in vitro.Blue ray wavelength ranged between 470nm and 520nm.And the light intensity was about 2000Lx.After exposure to blue light,we tested the proliferation of human retinal pigment epithelial cells by CCK-8 kit.And then expression of miR-103 was measured by the real-time PCR.RESULTS: Exposure to blue light inhibited the proliferation of human retinal pigment epithelial cells and increased the expression of miR-103.Moreover,up-regulation of miR-103 inhibited the proliferation of human retinal pigment epithelial cells,and down-regulates miR-103 promoted the proliferation of human retinal pigment epithelial cells.CONCLUSION: Blue light inhibits the proliferation of human retinal pigment epithelial cells by the up-regulation of miR-103.
8.P-VEP games aided combined treatment of amblyopia in children
Zheng, YIN ; Xiao-Ying, LI ; Ying-Qiao, KUANG ; Ting, LI ; Mu-Zhen, HUANG ; Xu-Guang, XIA
International Eye Science 2014;(8):1488-1490
AIM: To evaluate the efficacy of combined therapy for amblyopia in children by making use of pattern visual evoked potential ( P-VEP) game.
METHODS: This was a prospective case control study. These asthenopic children were divided into two groups. The control group ( 66 eyes of 49 patients ): occlusive therapy with glasses, cover, precision work, red light treatment and so on, later the stereo vision training was added. The experimental group (72 eyes of 52 patients):conventional methods mentioned above with P - VEP games.
RESULTS: The total effective rate and cure rate of experimental group in 6mo were higher than those of control group. The overall effective rate was 94. 4% in the experimental group and 83. 3% in the control group. There was a statistically significant difference between them (P<0. 05).
CONCLUSION: The comprehensive therapy by making use of P-VEP game is an individualized effective new way in treating amblyopia.
9.Matrix metallo proteinase-2 and focal adhesion kinase expression in herpes simplex virus-1 infected human corneal epithelial cell
Ting, CAO ; Yi-qiao, XING ; Yan-ning, YANG ; Hai-feng, MEI
Chinese Journal of Experimental Ophthalmology 2013;32(11):1050-1054
Background Previous studies showed that after herpes simplex virus-1 (HSV-1) infection of the cornea,matrix metallo proteinase-2 (MMP-2) (produced by corneal cells and corneal epithelial cells) plays an important role in the development of HSK.Focal adhesion kinase (FAK)plays an important role on the expression,release and activation of MMPs.This study explored the expressions of MMP-2 and FAK,which induced by HSV-1 infected human corneal epithelial cells.Objective To investigate MMP-2 and FAK expression in HSV-1 infected human corneal epithelial cells.Methods Human corneal epithelial cells were infected with HSV-1 in vitro to establish cell model of viral infection.The expression of MMP-2 and FAK were detected by reverse transcription PCR (RT-PCR),Western blot,immunohistochemical method and immunofluorescence method at 2 hours,20 hours and 40 hours after infection.Results At 2 hours,20 hours and 40 hours after infection,the expressionis of MMP-2 mRNA and FAK mRNA were significantly increased in comparison with uninfected cells (MMP-2 mRNA:Ftime =0.968,P=0.436 ; Fgroup =47.649,P =0.000 ; Fi ion =0.757,P =0.536.FAK mRNA:Ftime =0.658,P =0.631 ; Fgroup =35.182,P=0.000;Finteraction =1.386,P=0.137).Western blot assays showed that there were no significant differences in p-FAK,FAK or MMP-2 expressions between infected cells and control cells after 2 hours (P>0.05),but the expression levels of infected cells were significantly increased at 20 hours and 40 hours (both at P < 0.05).Immunohistochemistry results showed that longer infection time was associated with an increased number of cells staining for MMP-2,FAK and p-FAK.Conclusions At the initial stage of HSV-1 infected,p-FAK plays an important role in the process of virus invading and MMP-2 activation.
10.Practice and thought for quality control of drug clinical trial institution for clinical trials.
Ting ZHAO ; Ping WU ; Yong LI ; Feng-mei LIAN ; Ping LIU ; Jie QIAO
China Journal of Chinese Materia Medica 2014;39(24):4880-4882
The quality control of new drug cilnical trial is the effective guaranty for the pharmaceutical safety and effective after available on market. Enhancing the inspection and quality control of new drug clinical trials provide the crucial importance to achieve a persistent profitable standard. This paper mainly discussed the problems of current clinical trials based on annual check of drug clinical trial institution.
Clinical Trials as Topic
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Drug Evaluation
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Health Facilities
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Humans
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Quality Assurance, Health Care
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Quality Control