Objective:To analyse the actual position of denture base and its relationship with the anatomic marks of alveolar ridge. Methods:According to the standardized process,10 sets of complete dentures were fabricated for edentulous patients by taking func-tional impression.Before and after completion of denture fabrication,trial wearing and full adjustment one week after application,the margin extension,thickness and form of the denture bases were measured.The relationship of denture base outline with anatomic marks of alveolar ridges was observed.Results:The most adjusted regions of upper and lower complete denture bases were hamular notch area,buccal shed area,far middle buccal corner region and retromylohyoid ridge area(P <0.05).Buccal outline of mandibular base was consistent with the external oblique ridge and the base edge corresponding to masseter muscle attachment in far middle buccal corner region was excluded from the base.Lingual outline was relieved in area of lingual frenum and was consistent with mandibular torus.Conclusion:Denture base is easily over extended in hamular notch area,buccal shed area,far middle buccal corner region and retromylohyoid ridge area.

Objective To investigate the effects of sodium arsenite (NaAsO2) on cell survival circumstance,reactive oxygen species (ROS) and cell apoptosis in human normal hepatic cells (L-02).Methods L-02 cells were exposed to different doses of NaAsO2 (0,50,100,150 μmol/L) for 24 h.MTT assay was used to detect the survival of L-02 cells,and flow cytometry (FCM) was used to detect the ROS levels and the early (Q4),late (Q2) apoptosis of L-02 cells.Results Cell survival rate:cell survival rate was compared between groups,the difference was statistically significant (F =350.51,P ＜ 0.05),the cell survival rates of 50,100 and 150 μmol/L NaAsO2 groups [(87.30 ± 3.74)％,(49.03 ± 4.72)％,(13.44 ± 4.01)％] were significantly lower than that of the control group [(100.00 ± 0.00)％,all P ＜ 0.05];compared with 50 μmol/L NaAsO2 group,the cell survival rates of 100 and 150 μmol/L NaAsO2 groups were significantly decreased (all P ＜ 0.05);compared with 100 μmol/L NaAsO2 group,the cell survival rate of 150 μmol/L NaAsO2 group was significantly decreased (P ＜ 0.05).The ROS levels:ROS levels were compared between groups,the difference was statistically significant (F =407.78,P ＜ 0.05),the ROS levels of 100 and 150 μ mol/L NaAsO2 groups (3 212.00 ± 221.93,5 521.33 ± 179.63) were significantly higher than that of the control group (1 691.67 ± 73.98,all P＜ 0.05);compared with 50 μmol/L NaAsO2 group (1 927.67 ± 62.45),the ROS levels of 100 and 150 μmol/L NaAsO2 groups were significantly increased (all P ＜ 0.05);compared with 100 μmol/L NaAsO2 group,the ROS level of 150 μ mol/L NaAsO2 group was significantly increased (P ＜ 0.05).Cell apoptosis:cell apoptosis rates of Q2,Q4 and Q2 + Q4 were compared between groups,the differences were statistically significant (F =256.84,26.53,63.89,all P ＜ 0.05);excecpt the cell apoptosis rate of Q4 in 50 μ mol/L NaAsO2 group [(5.43 ± 0.57) ％],the cell apoptosis rates of Q2 [(5.67 ± 0.21)％] and Q2 + Q4 [(11.10 ± 0.40) ％] in 50 μ mol/L NaAsO2 group,the cell apoptosis rates of Q2 [(13.60 ± 0.79) ％],Q4 [(7.37 ± 2.01) ％] and Q2 + Q4 [(20.97 ± 2.38) ％] in 100 μmol/L NaAsO2 group,the cell apoptosis rate of Q2 [(13.47 ± 0.78) ％],Q4 [(16.97 ± 3.45) ％] and Q2 + Q4 [(30.43 ± 3.84) ％] in 150 μmol/L NaAsO2 group were significantly higher than those of the control group [Q2:(3.47 ± 0.12) ％,Q4:(2.90 ± 0.90) ％,Q2 + Q4:(6.37 ± 1.00) ％,all P ＜ 0.05];compared with 50 μmol/L NaAsO2 group,the cell apoptosis rates of Q2,Q4 and Q2 + Q4 in 100 and 150 μmol/L NaAsO2 groups were increased,except the cell apoptosis rate of Q4 in 100 μ mol/L NaAsO2 group,the differences were statistically significant (all P＜0.05);the cell apoptosis rates of Q4 and Q2 + Q4 in 150 μmol/L NaAsO2 group compared with 100 μmol/L NaAsO2 group were significantly increased (all P ＜ 0.05).Conclusions NaAsO2 can induce L-02 cells to increase ROS levels,and inhibit L-02 cell proliferation.In addition,NaAsO2 can induce early apoptosis and late apoptosis in L-02 cells.

Objective To explore the environmental risk factors,periconceptional folate intake and methylenetetrahydrofolate reduetase(MTHFR) gene C667T polymorphism of pregnant women on congenital heart diseases(CHD) in offspring.Methods Retrospective case-control study was carried out to investigate periconceptional folate supplementation and environmental factors in 98 parents with CHD offsprings and 101 parents with normal offsprings.The mothers'MTHFR gene C667T mutation was also identified.The possible risk factors were analyzed by simple and multiple factors Logistic regression methods.Results Six factors were related to the occurrence of CHD in the offspring:education of gestation mother,no prenatal examination,under depressed or nervous condition during pregnancy and maternal exposures to harmful substance;periconceptional folate and compound vitamin supplementation were protection factors.There were significant difference between case and control group in folate supplement(P

Ophthalmology is a predominantly morphology of clinical discipline. The eye has the characteristics of small scale, close structure, complex and diverse diseases, different clinical manifestations and so on. As an effective tool, the mind map can promote thinking visualization, help learners establish the logic relation of things and cultivate their innovative ability. To achieve better teaching effect, we introduce the mind map into theory course teaching of ophthalmology. On the one hand, teachers can optimize the teaching design, clarify the teaching ideas, and on the other hand, students can draw the mind map on their own or with others which will improve their subjective initiative of learning and study efficiency, and at the same time cultivate their ability of mutual collaboration.

Objective To explore the related factors on sore throat and pharyngeal xeransis during thyroid surgery.Methods Twenty-nine female patients, aged 24-67 years, BMI 18-30 kg/m2, falling into ASA physical status Ⅰ or Ⅱ, were scheduled for thyroid surgery.After anesthesia induction and tracheal intubation, the endotracheal intracuff was inflated to 20 mm Hg.Intracuff pressure was monitored every 5 minutes by a pressure transducer.At the time of 24 hours after tracheal intubation, the patients were asked about their throat complaints such as sore throat and pharyngeal xeransis.Results Endotracheal intracuff pressure during thyroid surgery was in a discrete distribution.Multiple linear regression model analysis found that age, BMI, anesthesia time and intracuff pressure had no obvious effects on sore throat.Decision tree model analysis found that patients undergoing thyroid surgery had higher probability of pharyngeal xeransis grade Ⅳ, when the average intracuff pressure was higher than 29 mm Hg.Conclusion Excessive endotracheal intracuff pressure during thyroid surgery due to operation causes pharyngeal xeransis.

Objective To explore the effect of early screening and psychotherapy on post stroke depression. Methods 80 patients with stroke at first onset were assessed with Hamilton Rating Scale for Depression (HAMD-17). Patients with depression were divided into control group (n=21) and intervention group (n=21). Both groups received rehabilitation therapy and the intervention group received psychotherapy in addition. They were assessed with HAMD again 2 months after treatment. All of them were evaluated with modified Barthel index (MBI). Results 52.5% patients had post stroke depression. The score of HAMD-17 was lower in the intervention group than in the control group (P<0.01). There was no significant difference in MBI between 2 groups after treatment (P>0.05). Conclusion Early screening and psychotherapy can facilitate the recovery of patients with depression after stroke.

Cardiomyopathy, Dilated ; pathology ; Cardiomyopathy, Hypertrophic ; pathology ; Diagnosis, Differential ; Fetal Diseases ; pathology ; Fetus ; Heart Neoplasms ; pathology ; Humans ; Myocardium ; pathology ; Ventricular Dysfunction, Left ; pathology

Objective To investigate the change of emotion and striatum dopamine transporter(DAT) expression in adult male rats experiencing maternal deprivation, and to explore whether DNA methylation is involved in the regulatory mechanism of DAT expression. Methods Newborn rats were randomly divided into 2 groups: a maternal separation group (n=16) and a control group (n=14). The maternal deprivation group were separated from their mother for 6 hours (09∶00-15∶00) per day from postnatal day 1 to 14, while the controls (n=14) without the deprivation. When the rats in the 2 groups were 12 week, their spontaneous anxiety levels and exploratory ability in novel environments were assessed by an elevated plus maze and an open field test. DAT mRNA expression in the striatum was detected by reverse transcription-PCR, and its DNA methylation level was measured by bisulfated DNA sequencing. Results Maternally-deprived rats showed lower ability of exploring in a new environment and lower levels of anxiety than the controls. The expression of DAT mRNA in the striatum of the maternal separation group (0.236±0.043) was significantly lower than that in the control group (0.480±0.107) (P＜0.05). However the DNA methylation level in the promoter region of DAT was not significantly different between the 2 groups. Conclusion Maternal deprivation influenced the emotion and expression of dopamine transporter in adult rats and DNA methylation may not be involved.

ObjectiveTo investigate the effects of isoflurane anesthesia on the expression of brain-derived neurotrophic factor (BDNF) and phosphorylated extracellular signal-regulated kinase (p-ERK) in neonatal rat hippocampus.MethodsForty-eight SD rats of both sexes,aged 7 days,weighing 12-17 g,were randomly divided into 2 groups ( n =24 each):control group (group C) and isoflurane anesthesia group (group Ⅰ).In group Ⅰ,the rats were exposed to2.5％ isotlurane for 3 min and then 1.5％ isoflurane was inhaled for 4 h,while in group C the rats were exposed to air for4 h.Arterial blood samples were collected immediately after anesthesia for blood gas analysis and for determination of the blood glucose concentration.Five rats in each group were sacrificed at 0,6,24 and 48 h after anesthesia (T1-4) and hippocanpi were removed for determination of the expression of potassiumchloride cotransporter 2 (KCC2),potassium-chloride cotrmsporter 1 (NKCC1),BDNF and p-ERK by Western blot.NKCC1/KCC2 ratio was calculated.ResultsAcid-base imbalance,hypoxemia and glycopenia were not found immediately after anesthesia in both groups.Compared with group C,KCC2 expression was significantly down-regulated and NKCC1/KCC2 ratio was increased at T3 and T4,and the expression of BDNF and p-ERK was dewn-regnlated at T1 and T2 in group Ⅰ (P＜0.05).There was no significant difference in NKCCI expression at each time point between groups Ⅰ and C ( P ＞ 0.05 )、ConclusionIsoflurane anesthesia delays the neuronal development in neonatal rat hippocampus through down-regulating the expression of BDNF and p-ERK.

Objective To study the impact of thalidomide on the immunological liver injury animal model in mice .Methods The immunological liver injury animal model was established in mice by injection of Bacillus Calmette-Guerin(BCG) and lipopolysaccha-ride (LPS) .These mice were randomly divided into five groups :normal control group ,model group ,positive medicine group (cyclo-phosphamide 100 mg/kg ,ip ,1 ,3 and 5 days after inoculation) ,Salle Lenalidomide (low ,medium and high dose group) ,and all of the mice were continuously administrated for 15 days ,the index of liver and spleen were detected ;Spectrophotometric was used to detect the levels of serum ALT ,AST and the content of MDA ,SOD ,GSH-Px in liver homogenates ;HE stain was used to exanimate the liver histopathology for the pathological grading and scoring of liver injury ;ELISA was used to measure the expression of TGF-β1 , IL-6 in liver homogenates ;RT-PCR was used to detect the mRNA expression of TNF-αin liver tissue .Results Thalidomide can re-duce the ALT ,AST ,MDA contents in these mice model ,increase the SOD ,GSH-Px activities and significantly reduce the index of liver and spleen of the mice with immunological liver injury (P<0 .05 or P<0 .01);also it can reduce the TGF-β1 ,IL 6 expression , inhibit TNF-αmRNA expression ,and reduce the damage degree of liver pathology .Conclusion Thalidomide can relieve the liver fi-brosis after immunological liver injury ,playing a protective role in the immunological liver injury mice model ,and its mechanism may be related to the inhibition of TGF-β1 and TNF-αexpression and the balance of cytokines .