The roles that DNA methylation play in the congenital heart disease have recently begun to come to light.The abonormal DNA meghylation pattern on key regulation gne during heart development,such as T-box,CHD7,LINE1,methylenetrahydrofolate reductase,changed the expression of those gene,and increased the incidence of the congenital heart disease.The DNA methylation could be determined by sequencing,methylationsensitive PCR and combined bisulfate restriction anylysis.To study the DNA methylation will not only reveal the mechanism of congenital heart disease,but also provide a new strategy for clinical diagnosis and treatment.

Humans ; Precursor Cell Lymphoblastic Leukemia-Lymphoma ; therapy

This paper probes into problems about the change of hospital economic interest and moral relation etc. brought by trying out medical insurance system in Zhengjiang, Jiujiang City. Also in points out that economic states have influence on ethical concept. Ethical concepts have a role on economic behaviour, which are two sides of cause and effect chain. For these reasons, medical insurance system is mutual movement results of economic & ethical factors, It can coordinate the relationships between hospital economic interest and morality embody medical service fairness and economic moral principles.

Systemic lupus erythematosus is a typical autoimmune disease commonly seen in childhood, the immunnopathogenesis of which is complicated. Along with the rapid advances of immunology and molecular biology, all the associated research has found that the onset of systemic lupus erythematosus is associated with dysfunction of T cells and B cells. They focus on the break of the normal autoimmune immunity and the form of malgenic autoantibody. It is also connected with primary immunodeficiency. The dysfunction of inherent immunity contributes to the break of autoimmune and the progression of the disease.

Objective:To analyze the relationship between PPD and MF on the basis of the surface antigen CD4,CD8 and receptor ? of T cell,so as to provide if PPD can represent a cutaneous lymphoid dyscrasia.Methods:The expression of CD4 and CD8 was measured by immunohistochemical technique in specimens obrained from lesional skin of 23 cases of PPD,20 cases of MF and skin of 20 cases of normal persons.The TCR-gamma gene rearrangement was detected with PCR.Results:15 cases of PPD have obvious migrations of lymphocytes to the epidermis.Their 5 cases and 20 cases of MF show T cell epidermotopism;18 cases of PPD,20 cases of MF and 6 cases of normal persons show a dominance of CD4+.In the dermis,the numbers of CD4+ between PPD and MF are 38.2?30.6 and 68.8?47.6(P

The theory of multiple intelligences put forward by Gardner has brought us enlightenment in different aspects.The theory maintains that every individual has eight intelligences,among which the advantageous one may be transferred to the others in a certain situation,and this greatly inspires multimedia EFL teaching at college.

To set up a health insurance program to facilitate a rational allocation of health re- sources,a scientific theory of health economics must be founded as a guidance and the percentage of the supply of health resources must be fixed in the GNP Consideration should be given to the ways in which the limited health resources are to be well allocated with the financial performance of the service units guaranteed at the same time.The current allocation of China's health resources indicates that the insuf- ficiency of national input go hand in hand with the false appearance of the ouersupply of such resources, which is called the"False Appearance Demand relationship"or"Nonensuring Line Demand Relationship".The divergence between the ensuring line and the nonensuring line shows the irrational waste-causing allocation of health resources.To help the establishment and improvement of China's health security system and make full and rational use of the health resouces,a social insuring line show- ing the regular total input of health resources muse be plotted;the macro—control function of the gov- ernment given full play;the reimbursement mechanism of medical cost improved;and the application of the law of value to the allocation of health resources instituted under the principle of fairness.

Objective To investigate the correlation between phlegm and humid type and blood lipid and blood glucose, and the countermeasures.Methods From August 2014 to September 2016, 162 patients with physical identification were selected as the subjects.According to the screening criteria, the patients were divided into phlegm constitution group and flat body constitution group.Fasting blood glucose(FPG), total cholesterol(TC), triglyceride(TG), high density lipoprotein cholesterol(HDL-C) and low density lipoprotein(HDL-C) were measured for all patients after fasting for 10-12 hours Cholesterol(LDL-C), and for analysis and comparison.Results The score of spleen deficiency was lower than that of phlegm constitution group, the difference was statistically significant(P<0.05).The level of blood glucose and blood lipid in the group was significantly better than that in phlegm constitution group(P<0.05).Conclusion The blood lipid, blood glucose level and spleen and stomach function of phlegm constitution are relatively poor, and the phlegm constitution can be improved by developing good living habits and combining spleen and dampness medicine.

Objective:To evaluate the literature quality of randomized controlled trials of acupuncture plus rehabilitation for post-stroke limb spasm in the recent ten years in China. Methods:By searching four databases of China National Knowledge Internet (CNKI), Wanfang Academic Journal Full-text Database (Wanfang), Chongqing VIP Database (CQVIP) and Chinese Biomedical Literature Database (CBM), randomized controlled trial (RCT) of acupuncture plus rehabilitation for the treatment of post-stroke limb spasm were screened and selected. In accordance with 2010 edition of the consolidated standards of reporting trials (CONSORT) and standards for reporting interventions in clinical trials of acupuncture (STRICTA), the quality evaluation was processed for RCT documents. Results:Finally, 50 documents were selected. The issues of titles, sample size calculation, application of randomization and blind method, subject’s procedure, conclusion and estimated value, explanation of trial limitation, auxiliary intervention measures and therapeutic plans, and background of therapists exist in most documents. Conclusion:Currently, RCT documents about acupuncture plus rehabilitation for the treatment of post-stroke limb spasm are generally of low quality. In the future, it is necessary to standardize the reports of the clinical trials based upon CONSORT and STRICTA, in order to enhance the document quality.

Objective To investigate the relation between macrophage infiltration and degree of calcific aortic stenosis (CAVS)in elder patients.Methods The aortic valve specimens were collected from 2012 September to 2014 March in Depart‐ment of Cardiac Surgery of Hainan General Hospital ,for operation removal ,from 80 cases[40 males and 40 females ,age:(58 ± 6)years old];normal aortic valve specimens were collected from 7 patients undergoing surgical removal of the normal aortic valve because of aneurysm[4 males ,3 female patients ,aged(41 ± 8)years] ,and 80 healthy adults served as control group.The clinical data of patients were collected ,and CAVS valve and normal valve were observed by HE staining.Expression feature of macrophages was studied by immunohistochemical(IHC)staining.IPP(Image‐Pro Plus)software was used to measure the densi‐ty of macrophages ,and Pearson relation analysis was employed to study the correlation between the density of macrophages and the degree of valve stenosis.Results As compared with healthy adults ,cholesterol and C reactive protein in patients with CAVS were significantly elevated.There were infiltration of inflammatory cells ,new blood capillaries and calcification in the valves of CAVS lesions observed by HE staining.No positive expression of macrophage marker CD68 was found in normal aortic valves , but CD68 positive expression was observed in the valves of CAVS by IHC staining ,and mainly concentrated in the peripheral calcification and osteoid tissues.Simple linear regression analysis showed the density of macrophages was positively correlated with the large pressure gradient(r=0.75 ,P<0.05) ,the average differential pressure(r=0.75 ,P<0.05) ,and the maximum aortic jet velocity(r=0.72 ,P<0.05) ,however ,was negatively correlated(r= -0.71 ,P<0.05)with the valve orifice area .Con‐clusion Compared with healthy adult volunteers ,the concentration of cholesterol and C reactive protein were elevated in blood of the patients with CAVS.Compared with the normal valve ,the macrophages were infiltrated in CAVS valves ;the density of macrophage was positively correlated with the degree of CAVS.