TG2 is a calcium-dependent enzyme that catalyzes the formation of covalent bonds between free amine groups in one protein and protein-bound glutamines of another,creating highly cross-linked protein complexes.TG2 is ubiquitous and most diverse member of the transglutaminase family of enzymes.TG2 are implicated in cell differentiation,receptor-mediated endocytosis,cell adhesion,and induction of apoptosis.Recently,a growing body of literature suggests that it could be closely related to the development of drug resistance.Its role in cancer biology is briefly reviewed in this paper.

Objective To study the effect of recombinant human thrombopoietin(rh TPO)for the treatment of post-chemotherapy thrombocytopenia in adult patients with acute myeloid leukemia(AML). Methods A total of 60 newly diagnesed AML patients ,law-and Intermediate-Rist with post-chemotherapy thrombocytopenia were randomly divided into experimental group (30 cases,rh TPO treatment)and control group(30 cases,no rhTPO treatment). Comparisons were made on the blood platelet recovery degree,the days of the blood platelet counting less than 20 × 109/L,the recovery days of the blood platelet counting more than 50 × 109/L,and incidence of adverse reac-tions of experimental group. Results After treatment,blood platelet recovery level between the experimental group and the control group has no difference(P>0.05). The days of the blood platelet counting less than 20 × 109/L and the recovery days of the blood platelet counting more than 50 × 109/L of experimental group were less than the control group separately(P < 0.05). The experimental group had low incidence of adverse reactions(6.7%). Conclusion rhTPO can not significantly increase the level of platelet recovery of AML patients after chemotherapy ,but can obvi-ously shorten the time of platelet recovery without obvious adverse reactions. The specific time needs further studied.

Acute hypoxic pulmonary vasoconstriction (HPV) plays an important role in physiological regulation. The purpose of this study was to explore the effects of Ilexonin A on hemodynamics and HPV, and their relation to prostaglandins (PG_(?)) and leukotrienes (LT_(?)).The results show that the high pulmonary vascular resistance induced by Ilexonin A in normoxic rats may be partially caused by the increased LTs and have no relation to PGs. Ilexonin A may potentiate HPV at least by two ways: replacing the production of PGs (especially PGI_2) in hypoxic Wistar rats, and increasing LTs production.

Animals ; Humans ; Occupational Exposure ; Organometallic Compounds ; poisoning ; toxicity ; Rats ; Toxicity Tests

To investigate the clinical effect of tonifying the kidney and promoting blood circulation to promote oocyte decoction in the treatment of anovulatory infertility caused by polycystic ovary syndrome. Sixty cases were selected from the out-patient department of Xiyuan hospital of China academy of Chinese medical sciences and the Chinese academy of traditional Chinese medicine, Chinese medicine out-patient department. Sixty patients with PCOS patients were randomly divided into the treatment group and the control group, with 30 cases and 30 cases respectively. The treatment group was given decoction of the reinforcing kidney, activating blood circulation and ovarian stimulation compound recipe. The control group was treated with clomiphene. Through the treatment of 1-2 courses, in the treatment group the pregnancy rate was 56.67%, the ovulation rate 61%; in control group of clomiphene citrate ovulation ratepregnancy rate was 30% , 72.84% of ovulation rate. The difference was significant between two groups (P < 0.05), the pregnancy rate in the treatment group was higher than the control group. The treatment group has regulatory effect on FSH, LH and their ratio, and increase E2 level, decrease T, PRL, INS and other hormone levels, contributing to the mature development of the follicles and endometrium growth, increase the ovulation rate and pregnancy rate. The control group on FSH, E2 increased, LH, T, PRL and INS showed no obvious effect.
Adult ; Drugs, Chinese Herbal ; pharmacology ; Female ; Fertility Agents, Female ; pharmacology ; Humans ; Infertility, Female ; drug therapy ; etiology ; Kidney ; drug effects ; Ovulation ; drug effects ; Ovulation Induction ; Polycystic Ovary Syndrome ; complications

With development of bio-technique, more and more proteins were applied as clinical approaches. However, the protein homogeneity, especially the N-glycosylation limited the further research and application of these protein drugs. The analysis method for N-glycans is believed to be critical in protein drugs development. To enhance the N-glycans isolation efficiency and accelerate the pretreatment, a new strategy was built on ultrafiltration-devices. New methods increased the isolation efficiency of N-glycans containing N-acetylglucosa mine with 10%-20%. The degrading of N-glycans containing sialic acids was also minimized with this method. 20%-100% more N-glycans with sialic acids were isolated. The pretreatment was finished within 30 min. Coupled with HPLC-HRMS, an effective and reliable strategy designed for protein drugs N-glycans analysis were developed.
Glycoproteins ; chemistry ; Glycosylation ; Polysaccharides ; chemistry ; Ultrafiltration ; instrumentation

Objective To evaluate cognitive function of chronic alcoholism patients.Methods The Wechsler Adult Intelligence Scale-Revise in China(WAIS-RC) and WMS-RC,including intelligence quotient,logical Memory.visual memory and stroop test,were investigated and P300 component of auditory event-related potential (ERP,P300),especially the subcomponents P3a and P3b were examined in 100 chronic alcoholism patients,and 70 healthv people matched in age,sex and educational level as controls.Results Compared with controls,the score of Verbal intelligence quotient(VIQ),Performance intelligence quotient (PIQ),Full-scale intelligence quotient (FIQ),logical memory,visual memory (immediate and delayed),and stroop test were significant lower (P <0.05.P＜O.01)in chronic alcoholism patients,but the latencies of P3a and P3b wave were significantly prolonged.the amplitude of P3a and P3b wave were significantly decreased in chronic alcoholism patients.Conclusion Chronic alcoholism patients have cognitive function impairment,and P300 is a more sensitive to discover the impairment of the cognitive function.

Rapeseed oil is one of the major raw materials for biodiesel production. The bottleneck of rapeseed oil-based biodiesel production at present is high cost of raw materials, which generally accounts for about 75% of total production cost. Current status and principal problems concerned in the production of biodiesel from rapeseed oil in China and other countries were introduced, based on which it was proposed to grow high-erucic acid rape in existing winter fallow fields and jointly produce biodiesel, erucic acid and its derivative products, and other highly valuable by-products such as glycerin and sterols from high-erucic acid rapeseed oil. The feasibility of this development strategy was analyzed in terms of product and market demand potential, economic benefits of enterprises, techniques and conditions of production, and source of raw materials. It was believed that the implementation of the strategy in China was of great significance in realizing rise and sustainable development of rapeseed oil-based biodiesel production, improving quality of biodiesel, promoting the development of biochemical engineering and many other industries, as well as in building a socialist new countryside.

Objective To explore the current situation of nursing students' leadership,in order to improve the clinical leadership of nursing students and provide reference for nursing teaching.Methods In July 2016,a questionnaire survey of nursing students including undergraduate and junior college students in our hospital was conducted by using the Self Assessment Leadership Instrument.SPSS 17.0 was used to conduct the t test to investigate the nursing leadership differences between the two groups.Results The total score of nursing students was (114.68 ± 18.25),and the score of undergraduate students was higher than junior college students (t=8.13,P=0.000);In the dimensions of critical thinking,decision-making skill,interpersonal relationship,team spirit and working relationship,there were significant differences in the scores of undergraduate and junior college students with different levels of education (P＜0.05).Conclusion The level of leadership of nursing students is low.In the process of nursing students' education,we should carry out the leadership courses in the school and practice leadership in clinical clerkship,so that we can promote the improvement of nurses' leadership and promote the development of nursing science.

Stressors can be classified as controllable and uncontrollable events.Self-choice behavior plays an important role in the "problem-solution" process of coping when individual faces controllable stressors.As to the uncontrollable stressors there are 2 different coping modes: transformation and compensation coping.This paper reviews the research achievements in the relationship between stress and self-choice behavior.