TG2 is a calcium-dependent enzyme that catalyzes the formation of covalent bonds between free amine groups in one protein and protein-bound glutamines of another,creating highly cross-linked protein complexes.TG2 is ubiquitous and most diverse member of the transglutaminase family of enzymes.TG2 are implicated in cell differentiation,receptor-mediated endocytosis,cell adhesion,and induction of apoptosis.Recently,a growing body of literature suggests that it could be closely related to the development of drug resistance.Its role in cancer biology is briefly reviewed in this paper.

Objective To study the effect of recombinant human thrombopoietin(rh TPO)for the treatment of post-chemotherapy thrombocytopenia in adult patients with acute myeloid leukemia(AML). Methods A total of 60 newly diagnesed AML patients ,law-and Intermediate-Rist with post-chemotherapy thrombocytopenia were randomly divided into experimental group (30 cases,rh TPO treatment)and control group(30 cases,no rhTPO treatment). Comparisons were made on the blood platelet recovery degree,the days of the blood platelet counting less than 20 × 109/L,the recovery days of the blood platelet counting more than 50 × 109/L,and incidence of adverse reac-tions of experimental group. Results After treatment,blood platelet recovery level between the experimental group and the control group has no difference(P>0.05). The days of the blood platelet counting less than 20 × 109/L and the recovery days of the blood platelet counting more than 50 × 109/L of experimental group were less than the control group separately(P < 0.05). The experimental group had low incidence of adverse reactions(6.7%). Conclusion rhTPO can not significantly increase the level of platelet recovery of AML patients after chemotherapy ,but can obvi-ously shorten the time of platelet recovery without obvious adverse reactions. The specific time needs further studied.

Acute hypoxic pulmonary vasoconstriction (HPV) plays an important role in physiological regulation. The purpose of this study was to explore the effects of Ilexonin A on hemodynamics and HPV, and their relation to prostaglandins (PG_(?)) and leukotrienes (LT_(?)).The results show that the high pulmonary vascular resistance induced by Ilexonin A in normoxic rats may be partially caused by the increased LTs and have no relation to PGs. Ilexonin A may potentiate HPV at least by two ways: replacing the production of PGs (especially PGI_2) in hypoxic Wistar rats, and increasing LTs production.

Objective: To establish a quantitative analysis of multi-components with a single-marker (QAMS) method for the determination of eight components in Qibai Pingfei Granule (QPG). Methods: Ginsenoside Rb1 was used as the internal reference substance. The relative correlation factors (f) of ginsenosides Rg, Re, Rf, Rc, Rb2, Rc, and astragaloside were calculated and established. The results were compared with those obtained by the external standard method in 10 batches of QPG. The results showed that the f values of ginsenosides Rg, Rf, Rc, Rb2, astragaloside, and ginsenosides Rc to ginsenoside Rb1 were 1.244, 1.075, 1.133, 1.090, 1.071, 0.967 and 1.070. Results: Ginsenosides Rg1, Re, Rf, Rb1, Rc, Rb2, Rd, and astragaloside had good relations within the ranges of 0.416 0-4.992, 0.315 4-3.785 2, 0.259 6-3.115, 0.385 2-4.622, 0.222 8-2.674, 0.193 2-2.318, 0.183 5-2.202, and 0.574 6-6.895 μg, respectively. The two methods did not show the significant difference in assay results. Conclusion: So the QAMS method is feasible and credible, and could be used to determine the multiple components in QPG.

The morbidity and mortality of gastrointestinal malignancies are the highest in the world. For patients with poor response to conventional chemotherapy, new treatment methods are urgently needed. In recent years, under the background of precision medicine, antibody-drug conjugates (ADCs) with high tumor specificity and potent toxicity have become a hot research spot in the field of biomedicine. However, due to the complex structure and mechanism of ADCs, its pharmacokinetic research is facing great challenges which are the biggest resistance to the development of ADCs at present. In this case, it is of great significance to understand the pharmacokinetic properties of ADCs and make use of it to improve the efficacy of ADCs in the treatment of gastrointestinal malignancies. Based on the basic composition and mechanism of ADCs, this review summarizes the pharmacokinetic properties of ADCs, discusses its recent advances in the treatment of gastrointestinal malignancies, in order to provide more references for follow-up research on ADCs.

Rapeseed oil is one of the major raw materials for biodiesel production. The bottleneck of rapeseed oil-based biodiesel production at present is high cost of raw materials, which generally accounts for about 75% of total production cost. Current status and principal problems concerned in the production of biodiesel from rapeseed oil in China and other countries were introduced, based on which it was proposed to grow high-erucic acid rape in existing winter fallow fields and jointly produce biodiesel, erucic acid and its derivative products, and other highly valuable by-products such as glycerin and sterols from high-erucic acid rapeseed oil. The feasibility of this development strategy was analyzed in terms of product and market demand potential, economic benefits of enterprises, techniques and conditions of production, and source of raw materials. It was believed that the implementation of the strategy in China was of great significance in realizing rise and sustainable development of rapeseed oil-based biodiesel production, improving quality of biodiesel, promoting the development of biochemical engineering and many other industries, as well as in building a socialist new countryside.

With development of bio-technique, more and more proteins were applied as clinical approaches. However, the protein homogeneity, especially the N-glycosylation limited the further research and application of these protein drugs. The analysis method for N-glycans is believed to be critical in protein drugs development. To enhance the N-glycans isolation efficiency and accelerate the pretreatment, a new strategy was built on ultrafiltration-devices. New methods increased the isolation efficiency of N-glycans containing N-acetylglucosa mine with 10%-20%. The degrading of N-glycans containing sialic acids was also minimized with this method. 20%-100% more N-glycans with sialic acids were isolated. The pretreatment was finished within 30 min. Coupled with HPLC-HRMS, an effective and reliable strategy designed for protein drugs N-glycans analysis were developed.
Glycoproteins ; chemistry ; Glycosylation ; Polysaccharides ; chemistry ; Ultrafiltration ; instrumentation

Objective To compare the effects of nasogastric tube and spiral nasointestinal tube on patients with severe brain injury. Methods Pa?tients receiving enteral nutrition with spiral nasointestinal tube or nasogastric tube were collected and investigated to evaluate the two schemes of en?teral nutrition from aspects of coma score,nutrition improvement,and catheter complications and so on. Results Detection of levels of total protein and prealbumin were conducted for all patients at 7 and 15 days after intubation. Each index was higher in the spiral nasointestinal tube group than in the nasogastric tube group. The reflux and aspiration rate was lower in the spiral nasointestinal tube group than in the nasogastric tube group. The dif?ferences were significant(P<0.05). Conclusion Using spiral nasointestinal tube to give enteral nutrition in patients with severe brain injury can improve the nutritional status,reduce complications,which is more contributory to the recovery.

To investigate the clinical effect of tonifying the kidney and promoting blood circulation to promote oocyte decoction in the treatment of anovulatory infertility caused by polycystic ovary syndrome. Sixty cases were selected from the out-patient department of Xiyuan hospital of China academy of Chinese medical sciences and the Chinese academy of traditional Chinese medicine, Chinese medicine out-patient department. Sixty patients with PCOS patients were randomly divided into the treatment group and the control group, with 30 cases and 30 cases respectively. The treatment group was given decoction of the reinforcing kidney, activating blood circulation and ovarian stimulation compound recipe. The control group was treated with clomiphene. Through the treatment of 1-2 courses, in the treatment group the pregnancy rate was 56.67%, the ovulation rate 61%; in control group of clomiphene citrate ovulation ratepregnancy rate was 30% , 72.84% of ovulation rate. The difference was significant between two groups (P < 0.05), the pregnancy rate in the treatment group was higher than the control group. The treatment group has regulatory effect on FSH, LH and their ratio, and increase E2 level, decrease T, PRL, INS and other hormone levels, contributing to the mature development of the follicles and endometrium growth, increase the ovulation rate and pregnancy rate. The control group on FSH, E2 increased, LH, T, PRL and INS showed no obvious effect.
Adult ; Drugs, Chinese Herbal ; pharmacology ; Female ; Fertility Agents, Female ; pharmacology ; Humans ; Infertility, Female ; drug therapy ; etiology ; Kidney ; drug effects ; Ovulation ; drug effects ; Ovulation Induction ; Polycystic Ovary Syndrome ; complications

Animals ; Humans ; Occupational Exposure ; Organometallic Compounds ; poisoning ; toxicity ; Rats ; Toxicity Tests