OBJECTIVETo observe the effect of Qingre Yangyin Recipe (QRYYR) on sex hormones and insulin resistance (IR) in polycystic ovary syndrome (PCOS) patients.

METHODSTotally 90 PCOS patients were randomly assigned to the Chinese herbs group,the Western medicine group, the combined group, 30 in each group. Patients in the Chinese herbs group took QRYYR, one dose per day in two portions, once in the morning and once in the evening. Patients in the Western medicine group took Metformin 500 mg, twice per day for 3 consecutive months. Patients in the combined group took QRYYR and Metformin (the same as the former said two groups) in the 1st month, and took QRYYR for the following two months. Fasting blood glucose (FPG) and postprandial 2 h blood glucose (2 h GLU) were determined using hexokinase method before and after treatment. Fasting insulin (FINS), postprandial 2 h insulin (2 h INS), luteinizing hormone (LH), follicle stimulating hormone (FSH), estradiol (E2), progesterone (P), prolactin (PRL), and testosterone (T) were detected using chemiluminescent method. Leptin and adiponectin (APN) were determined using ELISA. Homeostasis model assessment of insulin resistance (HOMA-IR) was calculated. Body weight and height were measured once before treatment and once after treatment to calculate body mass index (BMI). The total two-phase basal body temperature (BBT) actually obtained within 3 months was statistically collected to calculate the two-phase BBT rate. Scores for Chinese medical syndromes were compared between the two groups before and after treatment.

RESULTSCompared with before treatment in the same group, BMI, FINS, 2 h INS, HOMA-IR, leptin, LH, PRL, T, and scores for Chinese medical syndromes obviously decreased, and APN levels increased (P < 0.05). FPG and 2 h FPG obviously decreased in the Western medicine group and the combined group (P < 0.05). E2 levels obviously decreased in the combined group with statistical difference (P < 0.05). Compared with the Chinese herbs group, the difference of BMI between pre-treatment and post-treatment was more in the combined group (P < 0.05). The difference of FPG,2 h GLU, 2 h INS, HOMA-IR, and APN between pre-treatment and post-treatment was more in the Chinese herbs group and the combined group (P < 0.05). Compared with the Western medicine group, the difference of PRL, T, and scores for Chinese medical syndromes was more in the Western medicine group and the combined group (P < 0.05); the difference of E2 and LH was even more in the combined group (P < 0.05). Compared with the combined group, the biphasic rate was obviously lowered in the Western medicine group (P < 0.05).

CONCLUSIONSQRYYR could improve IR but with weaker power to that of Metformin. It also could decrease serum levels of LH, T, PRL, and scores for Chinese medical syndromes, with superior effect to that of Metformin. The effect in the combined group was better.

Adiponectin ; Blood Glucose ; Body Mass Index ; Drugs, Chinese Herbal ; pharmacology ; therapeutic use ; Estradiol ; Female ; Follicle Stimulating Hormone ; Gonadal Steroid Hormones ; Humans ; Insulin ; Insulin Resistance ; Leptin ; Luteinizing Hormone ; Metformin ; Polycystic Ovary Syndrome ; drug therapy ; metabolism ; Progesterone ; Prolactin ; Testosterone

Breast Neoplasms ; metabolism ; pathology ; Female ; Humans ; Immunohistochemistry ; standards ; In Situ Hybridization ; standards ; Neoplasm Invasiveness ; Receptor, Epidermal Growth Factor ; analysis ; metabolism

CCL21 is originally reported as a CC chemokine mainly expressed in secondary lymphoid tissues. CCL21 can dictate lymphocytes homing in the flow environment,where T cells use integrins stimulated by CCL21 to arrest on endothelial cells,and can act as a chemoattractant for T cells,B cells,mature dentritic cells and natural killer cells strongly. CCL21 can collect activated effector cells and keep them around the tumor. It is also a pivotal molecule for priming T cell responses. Therefore,CCL21 plays a potential role in anti-tumor therapy, and its high expression is responsible for many immune diseases.

Objective To observe the clinical efficacy and security of anti-tuberculosis combined with highdose methylprednisolone in treatment of advanced tuberculous meningitis.Methods 77 patients with advanced tuberculous meningitis in our department were randomly divided into the observation group(39 cases)and the control group (38 cases).All of the patients were given initiate antiphthisie treatment,the control group were added dexamethasone,20mg/d,iv drip,then was maintained after improved;the observation group were added high-dose methyprednisolone(500mg/d)by intravenous injection for five days,then was given methyprednisolone 80mg/d to maintain.Two groups were treated with hormone no more than three months.Then the clinical efficacy and adveme reaction were compared.Results The observation group:32 cases of healing,6 cases of effective,1 case of inefficient,the total effective rate was 97.4%and the control group were 22,8,8,78.9%;the difference of total effective rates in the two groups was statistically significant(x2=7.57,P＜0.05).The time of consciousness restoration and defevesence,the white blood cell count,protein chloride and glucose content of cerebrospinal fluid of the observation group were(2.7±1.3)d,(13±5)d,(112.37±27.57)×106/L,(3.04±0.82)g/L,(32.7±6.4)mmol/L and(1.67±0.65)mmol/L;and the control group were(5.2±2.4)d,(19±6)d,(93.74±20.18)×106/L,(1.92±0.64)g/L,(25.4 ±5.2)mmol/L and(1.04±0.34)mmol/L,the difference was statistically significant(t=5.70,4.77,3.88,6.67,5.48,5.31,P＜0.01);The difference of adverse reaction rate between the two groups was not statistically significant (P＜0.05).Conclusion The clinical efficacy of anti-tuberculosis combined with high-dose methylprednisolone in treatment of advanced tuberculous meningitis was better and had advantages in improving symptoms and signs without enhancing the rate of adverse reaction and it was worth to promote in clinical.

Pneumonia is the primary cause of death in children under five-years old.About 2 million children younger than 5 years die of pneumonia each year worldwide,accounting for 19％ of the total number of deaths.According to the survey in 2008,7％ to 13％ of the children with pneumonia were severe pneumonia.Currently,the early diagnosis and treatment of severe pneumonia is often difficult.In order to improve the early diagnosis an prognosis cognition level of severe pneumonia,here is to make a review on the recent literatures on the biomarkers like brain natriuretic peptide,D-Dimer,C-reactive protein,lactate,zinc,procalcitoin,prealbumin,platelet count,endothelin-1 and high mobility group box 1 in children with severe pneumonia.

A series of changes of maternal thyroid hormones occur during pregnancy.The growth and development of offspring,especially the growth of the thyroid gland,depends a lot on maternal normal thyroid function.If the thyroid dysfunction of pregnant women is not diagnosed and treated properly,the children may suffer with serious consequences,including fetal loss (abortion,stillbirth),premature birth,congenital malformation,hyperthyroidism or hypothyroidism,delayed development of the nervous system,etc.Maternal thyroid dysfunction even influences the long-term growth of offspring.Therefore,we should detect and control thyroid dysfunction of pregnant women and their children as early as possible,in order to improve the survival rate and life quality of the offspring.

Acute respiratory distress syndrome is one of the common respiratory diseases in neonates. It is more common in neonates by elective cesarean section.The pathogenesis is complicated, while delayed lung fluid clearance is considered playing a role in it.Meconium aspiration, delayed establishment of respiratory reflex, gestational age, contractions before onset, male baby,perinatal asphyxia,maternal diabetes or asthma are thought to be risk factors.

The long bones of the fore and hind limbs of 1-day and 5-day new born mice werefixed in Zenker's 10% neutral formalin and Carnoy's fixatives. They were sectionedwithout decalcification and stained in Mallory for general structure, in McLean & Bloom'smodification of Kossa for calcification and in periodic acid-Schiff's reagent for glycogenrespectively. It was found that in the humerus the length of the cartilaginous cell columns, thedegree of calcification in the cartilaginous matrix and the extension of the subperiostealcollar in the cartilaginous level were all more extensive in the proximal end than that of thedistal. The situation was the opposite in the case of femurus. The development in theproximal ends of the radius and ulna were less extensive than the distal. On the otherhand, the proximal ends of the tibia and fibula were more extensive than the distal. Hence,the increase in length of the fore limb would be mainly in the shoulder and wrist jointswhile that of the hind limb in the knee joint. The distribution of glycogen in the chondrocytes was found to be more abundantin the transitional zone between the flattened and hypertrophied cell columns. Thesignificance of this distribution in relation to phophatases, nutrition and calcification wasdiscussed.

Experimental myocardial lesions were produced in 79 adult rats by the application of electrocoagulator.The animals were killed at intervals from 12 hours to 60 days after the injury and the lesions were studied histologically and histochemically. Three stages can be distinguished during the process of wound-healing in the cardiac muscles. The first stage,12 hours to 2 days after injury.The retrogressive changes of in- flammation were apparent.Degeneration and necrosis of heart muscles were present in the area of the injury.Young connective tissue were formed. The second stage,4 to 13 days after injury.The newly formed cardiac muscle fibers retroceded.The scar was formed of ripe connective tissue. After injury,the change in glycogen content in the cardiac muscle fibers was more conspicuous of than that of the desoxyribose nucleic acid.

AIM: To estimate the efficacy of milrnone in treatment of congestive heart failure. METHODS: 122 cases of chronic congestive heart failure with different etiology were divided into milrinone group and dabutamine group randomly. Milrinone group accepted milrinone (10 mg?d -1 ) for 5 days, and dabutamine group accepted dabutamine (160 mg?d -1 ) for 5 days. The efficacy of treatments was evaluated. RESULTS: The effectiveness rates of cardiac function improvement were 93.9 % and 76.7 % in milrinone group and dabutamine group, respectively (P