2.Detection of fungi in liquor workers with tinea corporis and tinea cruris using arbitrarily primed polymerase chain reaction.
Dan YAN ; Liao LI ; De-yu CHEN ; Yu-hua ZHANG ; Chao-hui HU ; Zheng-hua DENG
Chinese Journal of Industrial Hygiene and Occupational Diseases 2007;25(3):133-135
OBJECTIVETo explore the method of rapid detection of skin fungi and the significance of conventional diagnosis liquor worker tinea corporis and tinea cruris using arbitrarily primed polymerase chain reaction AP-PCR.
METHODSAmong liquor workers who were 50 tinea corporis patients, 58 tinea cruris patients and 50 health persons, we amplified the DNAs of the dermatophytes were amplified using AP-PCR and random primers OPD18 5'-GAGAGCCAAC-3' and OPAA11 5'-ACCCGACCTG-3', at the same time, the dermatophytes with microscope were detected and cultured.
RESULTSAP-PCR analysis detected fungal DNA in 45 patients(90.00%) among 50 liquor worker patients with tinea corporis, 31 patients(62.00%) had the positive results of microscope detection, and 41 patients(82.00%) had the positive results of standard culture. Among these workers who suffered from tinea corporis, T.rubrum, T.mentagrophyte, M. canis and E.floccosum were detected by AP-PCR. T.rubrum, T.mentagrophyte and M.canis were detected by standard culture. AP-PCR analysis detected fungal DNA in 53 patients(91.38%) among 58 liquor worker patients with tinea cruris, 37 patients(63.79%) had the positive results of microscope detection, and 48(82.76%) had the positive results of standard culture. Among the 58 workers who had tinea cruris, T.rubrum, E.floccosum and T.mentagrophyte were detected by AP-PCR and standard culture. Among 50 health persons, AP-PCR analysis detected fungal DNA in 3 persons(6.00%). The detection result with AP-PCR indicated that the kinds of fungi were T.rubrum and T.mentagrophyte. No one health person had the positive result in detection of fungi using microscope detection. Only one(2.00%) health person was detected to be infected by fungus with cultural way. The kind of fungus was T.rubrum.
CONCLUSIONAP-PCR is a rapid, sensitive and specific detection method for occupational dermatophyte patients. It can be used to detect and diagnose professional dermatophytosis.
Adolescent ; Adult ; DNA Primers ; Female ; Humans ; Male ; Middle Aged ; Occupational Diseases ; diagnosis ; microbiology ; Polymerase Chain Reaction ; methods ; Sensitivity and Specificity ; Tinea ; diagnosis ; etiology ; microbiology ; Young Adult
3.Tinea Incognito in Korea and Its Risk Factors: Nine-Year Multicenter Survey.
Won Jeong KIM ; Tae Wook KIM ; Je Ho MUN ; Margaret SONG ; Hoon Soo KIM ; Hyun Chang KO ; Byung Soo KIM ; Chun Wook PARK ; Seok Jong LEE ; Mu Hyoung LEE ; Kyu Suk LEE ; Young Chul KYE ; Kee Suck SUH ; Hyun CHUNG ; Ai Young LEE ; Ki Ho KIM ; Sook Kyung LEE ; Kyoung Chan PARK ; Jun Young LEE ; Jee Ho CHOI ; Eun So LEE ; Kwang Hoon LEE ; Eung Ho CHOI ; Jong Keun SEO ; Gwang Seong CHOI ; Hai Jin PARK ; Seok Kweon YUN ; Seong Jun SEO ; Tae Young YOON ; Kwang Ho KIM ; Hee Joon YU ; Young Suck RO ; Moon Bum KIM
Journal of Korean Medical Science 2013;28(1):145-151
Tinea incognito (TI) is a dermatophytic infection which has lost its typical clinical appearance because of improper use of steroids or calcineurin inhibitors. The incidence of TI is increasing nowadays. We conducted retrospective review on 283 patients with TI from 25 dermatology training hospitals in Korea from 2002-2010 to investigate the demographical, clinical, and mycological characteristics of TI, and to determine the associated risk factors. More than half (59.3%) patients were previously treated by non-dermatologists or self-treated. The mean duration of TI was 15.0 +/- 25.3 months. The most common clinical manifestations were eczema-like lesion, psoriasis-like, and lupus erythematosus-like lesion. The trunk and face were frequently involved, and 91 patients (32.2%) also had coexisting fungal infections. Among 67 isolated strains, Trichophyton rubrum was the most frequently detected (73.1%). This is the largest study of TI reported to date and the first investigational report concerning TI in Korea. We suggest that doctors should consider TI when a patient has intractable eczema-like lesions accompanied by tinea pedis/unguium. Furthermore, there should be a policy change, which would make over-the-counter high-potency topical steroids less accessible in some countries, including Korea.
Adolescent
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Adult
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Aged
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Aged, 80 and over
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Child
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Child, Preschool
;
Demography
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Eczema/pathology
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Face/pathology
;
Female
;
Humans
;
Lupus Erythematosus, Cutaneous/pathology
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Male
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Middle Aged
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Psoriasis/pathology
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Republic of Korea
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Retrospective Studies
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Risk Factors
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Tinea/*diagnosis/microbiology
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Trichophyton/isolation & purification
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Young Adult
4.Skin Infection due to Trichophyton tonsurans Still Occurs in People in Korea but not as Outbreaks.
Weon Ju LEE ; Hyun Bo SIM ; Yong Hyun JANG ; Seok Jong LEE ; Do Won KIM ; Jae Bok JUN ; Yong Jun BANG
Journal of Korean Medical Science 2016;31(2):296-300
Since 1995, Trichophyton tonsurans has been one of the causative agents of dermatophytosis in Korea. Herein we evaluate 77 patients infected with T. tonsurans who visited an outpatient clinic between 2004 and 2014. Infections due to T. tonsurans were diagnosed by mycological examination, which included direct microscopic examination using 15% KOH and culture in potato dextrose agar complemented with 0.5% chloramphenicol. The annual prevalence of infection due to T. tonsurans was the highest in 2014 (15 cases) but remained constant in non-gladiators between 2004 and 2014. The ratio of male to female patients was 1:0.3. The spring season presented the highest incidence compared with other seasons, with 27 cases. The incidence of infections due to T. tonsurans among gladiators was highest in spring compared with the other seasons whereas the incidence in non-gladiators was the highest in the winter. The body site most commonly affected was the face. Tinea corporis was the most common subtype of dermatophytosis caused by T. tonsurans. Herein, we demonstrate that the prevalence of infection with T. tonsurans remain constant throughout the study period in Korea.
Adolescent
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Adult
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Aged
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Child
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Child, Preschool
;
Disease Outbreaks
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Female
;
Humans
;
Infant
;
Male
;
Middle Aged
;
Prevalence
;
Republic of Korea/epidemiology
;
Retrospective Studies
;
Seasons
;
Tinea/*diagnosis/epidemiology/microbiology
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Trichophyton/*isolation & purification
;
Young Adult