In order to understand the porcine epidemic diarrhea virus (PEDV) origin and variant characteristics in Liaoning province,diagnosed by PCR,separated by Vero cell,and identified by cell pathological observation,RT-PCR and S gene sequence analysis,1 PEDV strains (LN-2015-1) was successfully isolated from a pig farm of Liaoning province.Analysis of S gene sequence showed that compared withCV777 strain,there were the longest 9 bp insertion,6 bp deletion and 13 bp continuous mutation in addition to point mutation.There also were the longest 3 AA insert,2 AA deletion,and 3 AA or more continuous mutation.The epitope analysis showed that there were 16AA mutations in the 5 epitope regions.Homology analysis show that it had the highest sequence similarity of 99.2％ with HB-HA2015 strain,higher sequence similarity of 98.5％-98.8％ with the domestic and foreign representative strains isolated since 2010,and lower sequence similarity of 93.2％-95.6％ with the traditional strain isolated before 2010;the phylogenetic analysis showed that LN-2015-1 was clustered into the same group with home and abroad variation strain in recent years,and formed a small subgroup with HB-HA2015 at the same time.The evolutionary distance was far from the traditional strains.

Objective: To investigate the relationship between interleukin-6(IL-6) polymorphism and susceptibility to hepatocellular carcinoma(HCC) and clinical prognosis after hepatitis B. Methods: From September 2016 to September 2017, 100 patients with HCC after hepatitis B admitted to Linfen People's Hospital were selected as the observation group, and 100 healthy people were selected as the control group.Peripheral blood samples of the two groups were collected.IL-6 gene polymorphisms were detected to explore the relationship between IL-6 gene polymorphisms and the risk of HCC after hepatitis B. Logistic regression analysis was used to explore the risk factors of clinical prognosis in patients with HCC after hepatitis B. Results: The genotype C/T, T/T ratio and allele T ratio of IL-6 rs13419896 locus in the observation group were higher than those in the control group(all P<0.05). The C/C genotype of occasional smoking was used as the reference.The risk ratio of HCC was 1.92, the risk ratio of combined family history of HCC was 2.01, the risk ratio of frequent smoking was 2.16.Family history of HCC (P=0.023) and rs13419896(T/T)(P=0.017) were independent risk factors for death, metastasis or recurrence. Conclusion IL-6 gene rs13419896 carrying T gene is a risk factor for HCC, and genotype(T/T) is a risk factor for poor prognosis in HCC patients.