Objective? To understand the career maturity and professional identity among secondary and higher vocational run-through education nursing interns and to analyze the correlation between them. Methods In November 2018, we selected 84 nursing students from two "4+2" secondary and higher vocational run-through education classes in a higher vocational nursing college in Beijing as subjects by purposive sampling. All of the students were investigated with the Career Maturity Inventory(CMI)and Professional Identity Questionnaire for Nurse Students(PIQNS). A total of 84 questionnaire were sent out and 82 valid of them were collected. Results? Among 82 nursing interns, the total scores of CMI and PIQNS were (118.24±16.29) and (74.99±6.74). Career maturity had a positive correlation with professional identity (r=0.416,P＜ 0.01). The influencing factors of career maturity included independence of career choice and professional self-image among nursing interns (P＜0.01). Conclusions Nursing students have high levels of career maturity and professional identity. Educators should pay attention to the development of professional identity of them so as to improve their career maturity.