1.Surgical treatment of the intracranial subarachnoid cysts
Journal of Vietnamese Medicine 1999;232(1):179-181
Diagnostic work-up and management of intracranial arachnoid cysts are still controversial. The authors have reported one case of intracranial arachnoid cyst in association with epilepsy. Operative method: craniotomy with penetration.
Subarachnoid Hemorrhage
;
Surgery
;
Epilepsy
2.Results of treatment of hypersympathicotonic hyperhydrosis by percutaneous injection of hot saline into the thoracic sympathetic ganglia
Journal of Vietnamese Medicine 1999;232(1):194-197
164 patients with primary hyperhydrosis underwent bilateral upper dorsal “sympathectomy” by percutaneous injection a hot serum. Pretreatment epidemiological data are described. The immediate and late results, as well as the complications and side effects are detailed: For severe cases of hyperhydroisis that cause social, professional and emotional embarassment “sympathectomy” by percutaneous injection of hot serum is a procedure of choice due to good results and minor complications.
Ganglia, Sympathetic
;
Sympathectomy
3.Clinical feature and surgical treatment of neurinomas and spinal meningiomas
Journal of Vietnamese Medicine 1999;232(1):127-132
The authors reported results obtained in a series of 34 patients operated on for Spinal meningiomas and neurinomas (23 neurinomas and 11 meningiomas). They are critically analyzed. This series was similar to those of other authors with regarding to age, sex, location of the tumor and clinical presentation. Before surgery there were 3/34 patients with radical compression: pain only (local, radicular) pain and slight motor deficit, 11/34 patients (32.55%) with severe motor deficit, sensory deficit or severe motor deficit. 20/34 patients with paraplegia, severe sensory deficit and sphincter deficits. Posoperative results: excellent 8; good 6; fair 4, bad 2 and dead 1.
Neurilemmoma
;
Meningioma
4.Production, purification and characterization of novel protease from Bacillus amyloliquefaciens D19 isolated in Vietnam
Tan Viet Pham ; Hanh Thi Dieu Nguyen ; Thi Luyen Bui ; Ngoc An Nguyen
Malaysian Journal of Microbiology 2024;20(no.1):85-93
Aims:
Microorganisms play a vital role in the breakdown of natural organic compounds and are valuable objects for worldwide enzyme production. The aim of this study was to identify favorable production conditions for Bacillus amyloliquefaciens D19 protease, followed by the purification and chemical characterization of this novel enzyme to assess its potential applications in various fields.
Methodology and results:
In this study, favorable conditions of protease production from B. amyloliquefaciens D19 were determined using a medium containing soluble starch (1.5%), earthworm extract (1.0%), yeast extract (0.5%), NaCl
(1.0%), at pH 7.0-8.0, 37 °C for 36 h with 150 rpm shaking condition. The protease was purified and had a molecular weight of about 23 kDa. The optimum condition for casein hydrolysis was at 40 °C and pH 6.5-7.0 in the presence of 1.0 mM Na+ or 5.0 mM Zn2+. The enzymatic activity was maintained at 75-100% at 30-50 °C and in pH 6.0-10.0. The values of Vmax and KM were also determined as 1547 U/mg and 6.33 mg/mL, respectively.
Conclusion, significance and impact of study
The identified optimal conditions will serve as the foundation for the production of the 23 kDa B. amyloliquefaciens D19 protease, one of the smallest proteases within the Bacillus genus. Moreover, its notable heat resistance, broad pH tolerance, high substrate catalysis and moderate substrate binding affinity make this enzyme a promising candidate for various applications in the food-feed and brewing industries.