Objective To explore whether storage time and temperature influence the measurement of blood clotting functional parameter,in order to ensure accuracy of result.Methods 60 anticoagulated blood samples were selected.The levels of APTT,PT,TT and FIB were detected immediately after separated plasma,at 1 h,2h,and 4h after keeping at room temperature,at 4h,12h,24h after keeping at-4℃.Part of anticoagulated blood samples were separated plasma and detected 2h after keeping at room temperature.Results Compared to measurement for immediately separated plasma,APTT,PT,TT and FIB showed no differences for 1 h,2h after keeping at room temperature and 4h,12h,24h after keeping at-4℃.But the result for 4h after keeping at room temperature showed bad accuracy.Conclusion In the measurement of coagulation indices,after collecting blood sample,separating plasma should be conducted as soon as possible.The separated plasma should be measured within 2h after keeping at room temperature and 24h after keeping at-4℃.

Objective To investigate the myocardial injury induced by bile duct obstruction and the protective effects ulinastatin(UTI). Methods Dynamic observation of of the levels of malondialdyhyde(MDA) and superoxide dismutase(SOD) of myocardial tissues, and the levels of serum TBil, alkaline phosphatase(ALP), MB isoenzyme of creatine kinase(CK MB), endotoxin (ET), and tumor necrosis factor(TNF ?) in bile duct obstruction(BLD) rats and UIT treatment rats. The left ventricles of the rats were obtained for light and electronic microscopic observation . Immunohistochemical staining method of ABC used was to locate the expression and distribution of TNF ? in myocardial tissues. Results After ligation of the common bile duct, serum TBIL, ALP, CK MB, ET, TNF ? levels and myocardium MDA gradully increased, while SOD levels gradually decreased, and the expression of TNF ? in myocardium increased. As compared with BDL group at the same phase, in UIT group, serum TBIL, ALP, CK MB, ET, TNF? levels and myocardium MDA in UTI treated groups decreased, while myocardium SOD increased, and the expression of TNF ? in myocardium decreased.Myocardial injuries of bile duct obstruction were aggravated as time progressed, and there were less myocardial injuries in UTI treated groups than in BDL groups at the same stage as shown with light and electronic microscopic observation.Conclusions UTI can effectively protect the myocardium from ET,TNF ? and free radical injury in bile duct obstucion rats.

Objective:To detect mutations of exons 5 and exons 8 of PTEN gene in oral squamous cell carcinoma(OSCC),and to explore the relationship between gene mutations and development of OSCC.Methods: Mutations of exons 5 and exons 8 of PTEN gene were detected by polymerase chain reaction-single strand conformation polymorphism(PCR-SSCP).Results: The whole sequences of exons 5 and exons 8 of PTEN gene of all cases of OSCC were expanded.There was no mutation in exons 5 and exons 8 of PTEN gene of all cases of OSCC.Conclusion: There is no mutation in exon 5 and exon 8 of PTEN gene in OSCC.This might show that there is no correlation between mutations in exon 5 and exon 8 of PTEN gene and the development of OSCC.

AIM To study toxicity of Safflor Yellew (SY) for vascular endothelic cell (VEC) and whether SY has antagonistic effect on platelet activating factor (PAF). METHODS Use light microscopical observation and crystal violet colorimetric method to study morphological and bioactivity change of HVEC and EVC 304 after incubated with PAF, SY, SY+PAF, Ginkolide,and Ginkolide+PAF. RESULTS Cultured with PAF 30 minutes, HVEC had histopathological changes: from fusiform, polygond squamous cell to ellipsoid, circular cell , not binding tightly, but separated by wide gap. As cultured with SY+PAF, cell's morphological changes just as same as PAF's group. And its bioactivity had tendency of decline. Either HVEC was incubated with SY (0.571 g?L -1 ) for 30 minutes or EVC 304 cultured with SY(1 143 g?L -1 ) for 4 hours, their morphology and bioactivity had changes. CONCLUSION SY do not inhibit PAF's injury effect. And high concentration SY has toxicity to cultured endothelic cell.

Objective To investigate the clinical value of the peripheral blood monocyte human leukocyte antigen-DR (mHLA-DR) for assessment of degree of severity and the diagnosis of acute pancreatitis (AP). Methods A case-control study was conducted. Eighty-six AP patients admitted to Shandong Liaocheng People's Hospital from June 2014 to May 2015 were enrolled. Patients were classified into four groups [mild (n = 33), moderate (n = 25), severe (n = 16), critical (n = 12)] according to the disease classification. Eighty healthy persons subjected to physical examination center of our hospital at the same time were served as controls. Acute physiology and chronic health evaluation Ⅱ (APACHE Ⅱ) scores in patients were estimated. Flow cytometry was used to measure the expression of the peripheral blood mHLA-DR, and the Pearson method was used to analyze the relationship between the level of mHLA-DR and the APACHE Ⅱ score. The receiver-operating characteristic curve (ROC) was plotted, and then the clinical value of the peripheral blood mHLA-DR was analyzed for the diagnostic value in AP patients. Results The expression of the mHLA-DR in patients with AP was significantly lower than that of healthy control group [(63.7±18.6)% vs. (86.4±8.3)%, t = 5.319, P < 0.001]. The expression levels of the mHLA-DR in mild group, moderate group, severe group, and critical group were (79.6±6.5)%, (66.4±9.4)%, (49.9±8.1)%, (32.5±12.0)%, respectively, and the APACHE Ⅱ score were 4.67±1.99, 5.88±2.05, 9.06±2.62, 12.33±3.96, respectively. Pair wise comparisons were statistically significant (all P < 0.05). The HLA-DR expression level in the peripheral blood of patients with AP was negatively correlated with the APACHE Ⅱ score (r = -0.695, P < 0.001). The area under the ROC curve (AUC) of mHLA-DR expression in peripheral blood for AP was 0.894 [95% confidence interval (95%CI) = 0.847-0.941, P < 0.001], and the cut-off point was 84.40%, with the sensitivity of 75.0%, the specificity of 90.7%, and the accuracy rate of 83.1%. The AUC of mHLA-DR expression for mild AP was 0.938 (95%CI = 0.889-0.987, P < 0.001), and the cut-off point was 72.70%, with the sensitivity of 87.9%, the specificity of 88.7%, and the accuracy rate of 88.4%. The AUC of mHLA-DR expression for severe and critical AP was 0.943 (95%CI = 0.881-1.005, P < 0.001), and the cut-off point was 57.85%, with the sensitivity of 84.0%, the specificity of 96.4%, and the accuracy rate of 90.6%. Conclusions The expression levels of the peripheral blood mHLA-DR in AP patients can reflect the degree of disease, and contribute to the diagnosis of AP. The value of mHLA-DR may be used as a new biological indicator in the diagnosis and assessment for the severity of AP.

Human T-cell leukemia virus type 1 (HTLV-1) is a retrovirus demonstrated to be associated with human disease. Infection by the HTLV-1 can cause T-cell leukemia (ATL) in adults. HTLV-1 bZIP factor (HBZ) is a viral protein encoded by the minus strand of the HTLV-1 provirus. Among the regulatory and accessory genes of HTLV-1, HBZ is the only gene that remains intact and which is expressed consistently in all patients with ATL. Moreover, HBZ has a critical role in the leukemogenesis of ATL. Here, we review the function of HBZ in the oncogenesis of HTLV-1 and its molecular mechanism of action.
Animals ; Basic-Leucine Zipper Transcription Factors ; genetics ; metabolism ; Carcinogenesis ; HTLV-I Infections ; pathology ; virology ; Human T-lymphotropic virus 1 ; genetics ; metabolism ; Humans ; Leukemia, T-Cell ; pathology ; virology ; Retroviridae Proteins ; genetics ; metabolism

Objective: To investigate the inhibitory effect of survivin antisense oligonucleotide (ASODN) on the expression of survivin gene and proliferation of human hepatocellular carcinoma cell line SMMC-7721. Methods: The 20 mer antisense oligonucleotide (ASODN) targeted to the promoter region of survivin mRNA was designed and synthesized. The expression of survivin gene in hepatocellular carcinoma cell line SMMC-7721 was blocked by means of ASODN transfection mediated by DOTAP liposomal reagent. The changes of survivin protein and mRNA expression after transfection were as-sessd by Western blot and in situ hybridization, respectively. The apoptotic rate was detected by flow cytometer. The changes of cell adherent rate, cell growth activity, and the inhibitory rate of cell growth were also studied. Results: The expression of survivin protein and mRNA were decreased markedly after survivin ASODN transfection. Meanwhile, the cell adherent rate also decreased markedly while the apoptotic rate increased markedly. Conclusions: Transfection of ASODN targeted to the promotor region of survivin mRNA by DOTAP liposomal transfection reagent could down-regulated the expression of survivin protein and mRNA significantly in 7721 cell line and inhibit the proliferation of cancer cells. Survivin could be an important target in the therapy of hepatocellular carcinoma.

Objective To establish HPLC fingerprint for Fructus Aurantii Immaturus.Methods The HPLC method was used with Diamonsil-C18 column (250 mm?4.6 mm,5 ?m),and a mixture liquid of acetonitrile-0.01% NaH2PO4 as mobile phase in a gradient elution.The HPLC fingerprint for 36 batches of Fructus Aurantii Immaturus was studied on their similarity,cluster,and principal components analyses.The common HPLC fingerprint of Fructus Aurantii Immaturus was established,which was studied with principal components analyses.Results Under the selected spectrum condition,the 36 batches of Fructus Aurantii Immaturus were classified into two groups based on the result of similarity,cluster,and principal components analyses.Conclusion This method is reasonable and reliable to the quality control of Fructus Aurantii Immaturus.

Objective To investigate the effect and possible mechanism of curcumin on the proliferation and apoptosis of cell strain of bladder carcinoma.Methods In curcumin group,T24 cells were treated with curcumin of 1,10,100 ?mol/L respectively,while no curcumin treatment in control group.The growth,apoptosis,AKT1 protein activity and mRNA level,caspase 3 content of T24 cells were observed by MTT,TUNEL,RT-PCR,immunoblot and image analysis technology 12,24 and 48 h after the commencement of curcumin treatment.Results In the curcumin group,the inhibition rate,apoptosis and the content of caspase 3 of T24 cells increased significantly.The activity of AKT1 protein decreased,but the mRNA level of AKT1 showed no changes.Conclusion Curcumin can inhibit the growth of T24 cells and induce the cell apoptosis,which mechanism is related to the inhibition of AKT1 signal pathway of T24 cells.