Objective To investigate the serological and molecular biology characteristics of ambiguous blood types a-mong voluntary blood donors in Putian,and to file records for relevant donors for future reference in case of their own trans-fusion needs or emergency donations to others.Methods A total of 68 593 blood samples from voluntary blood donors in Putian Central Blood Station between January 1,2019 and August 31,2023 were collected and tested for blood types using serological methods.ABO gene(including promoters,enhancers and seven exons as well as their flanking sequences and in-tron 6)and FUT1 gene testing were performed on ambiguous blood types.3D models were constructed using Chimira and Py-MOL software to predict the impact of gene mutations on enzyme structure.Results A total of 16 ABO subtypes were identi-fied by serological methods,with the highest detection rate as the para-Bombay phenotype(0.73 per 10 000),followed by the cisAB phenotype(0.44 per 10 000).Gene analysis revealed 12 cases with known mutations(4 cases of FUT1.01N.06/FUT1.01N.06,1 case of FUT1 01W.08/FUT1.01N.06,2 cases of A2.08/B.01,1 case of BA.02/O.01.01,1 case of A3.07/O.01.01,3 cases of cisAB.01/B.01),and key mutations were not found in 4 cases(2 cases of A1.02/B.01,2 cases of A1.02/O.01.02).3D molecular model analysis revealed that both A3.07 and FUT1.01W.08 allele can lead to a decrease in ac-tivity of the corresponding glycosyltransferases,resulting in the emergence of subtypes.Conclusion The most common phe-notype causing discrepancies in ABO blood type testing among voluntary blood donors from Putian is the para-Bombay phe-notype,with the most common allele being FUT1.01N.06.

Objective To explore the association of sedentary life style with risk of metabolic syndrome (MS) and diabetes mellitus type 2 (T2DM).Methods A total of 6 016 local residents aged 18 years or older in Fujian province were recruited by multi-stage stratified cluster sampling method in 2010-2011.Data,including demographic information,physical activity and sedentary time were collected.Indices related to height,weight,waist circumference,blood pressure and blood lipid were determined while MS and T2DM were diagnosed by IDF (2005) and WHO (1999) criteria.Logistic regression was used to estimate the correlations between sedentary behavior and MS or T2DM.Results The prevalence rates of MS and T2DM were 19.0％ and 8.0％ respectively,in local residents aged 18 years or older,in Fujian province.The overall rate of sedentary behavior was 18.1％,with the mean sedentary time as 4.3 hours.Both data showed significantly differences (P＜0.001) among control group,MS without T2DM group,MS with T2DM group and T2DM without MS group.Compared with the group of sedentary time ＜2.0 h/d,1) the group with 2.0-3.5 h/d was significantly correlated with MT group (OR=1.44,95％ CI:1.03-2.03,P＜0.05),2) groups of 3.5-6.0 h/d and ≥6.0 h/d were significantly correlated with M,T,MT group,respectively (OR:1.49-1.76 and 1.28-1.58 respectively,95％ CI:1.19-2.45 and 1.02-2.23 respectively,P＜ 0.05),and 3) sedentary behavior was independently associated with an increased risk of MT group (OR=1.82,95％ CI:1.33-2.48,P＜0.01) and M group (OR=1.43,95％CI:1.14-1.78,P＜0.01),after the adjustment for factors as age,sex,cigarette smoking,alcohol drinking,BMI,education,occupation,sedentary behavior/sedentary time.Conclusion MS and T2DM were associated with sedentary lifestyle,but these findings should be confirmed through further longitudinal studies.

Objective:To investigate the blood pressure control and its influencing factors in hypertension patients with MS.Methods:Between January 2017 and December 2018, more than 78 000 residents aged 35-75 years selected through convenient sampling were invited to participant in China Patient-Centered Evaluative Assessment of Cardiac Event Million Persons Project in Fujian province, physical and laboratory tests were conducted for them, and their basic information were recorded. A total of 5 281 hypertension patients with MS were included in the study.Results:The treatment rate of hypertension patients with MS was 55.5%, and the control rate was 7.2%. The control rate was higher in patients who were older, women, had advanced education level, had history or family history of cardiovascular disease. The results of multivariate analysis indicated that living area (urban or rural), cardiovascular history, diabetes, urine protein, BMI had impacts on both treatment and control of hypertension. Family history of cardiovascular disease, age, self-management of hypertension, dyslipidemia, waist circumference and drinking had impacts on the treatments, and gender had effects on the control.Conclusions:The treatment rate of hypertension patients with MS was unsatisfactory and the control rate was low. Intervention should be strengthened in rural area, males and young age groups, and activity of self-management group of hypertension should be conducted regularly.