1.P gene RT region mutations of hepatitis B virus in 74 patients with lamivudine-resistance
Zhongdong ZHANG ; Huimin LIU ; Tiefeng Lü
Chinese Journal of Clinical Infectious Diseases 2008;1(4):204-206
Objective To investigate mutations at HBV P gene RT region in lamivudine-resistant patients. Methods Serum samples were collected from 74 hepatitis B patients resistant to lamivudine. HBV P gene RT region was amplified by PCR, and the PCR products were directly sequenced and the viral loads were detected. Results There were 7 forms of mutations in 74 patients harboring YMDD mutations detected in this study. The common mutation forms were rtM204V/rtL180M, rtM204I and rtM204I/rtL180M. The most frequent mutation positions were rtM204I (38.7%), rtM204V (21.8%), rtL180M (38.7%) and rtV173L (0.8%). Conclusions The mutation forms and positions of lamivudine-resistant viral strains are complex. Common mutation forms and positions are not directly correlated with the viral load, but some novel forms may be associated with it.
2.Exploration in constructing centers for clinical medicine in Shanghai
Kan ZHANG ; Qingshi XIA ; Tiefeng XU ;
Chinese Journal of Hospital Administration 1998;0(11):-
In order to maintain and develop the overall advantages and distinguishing features of medicine in Shanghai, the citys health system implemented the program of constructing centers for clinical medicine. The program involved not only the optimization and integration of health resources in the whole city but also the structural readjustment and resources regrouping within the citys health system and each individual hospital. After more than two years construction, 33 centers for clinical medicine have been set up in the city, resulting in primary achievements in expertise improvement, effect of scale, and quality benefits. By analyzing the hardware and software construction process, it can be seen that the centers for clinical medicine have made primary achievements in such aspects as ideological construction, work efficiency, interdisciplinary cooperation, academic status enhancement, medical quality improvement, and reform in the system of personnel distribution.
3.Tissue culture and rapid regeneration of Hypericum perforatum
Ruxian DING ; Tiefeng XU ; Hanming ZHANG ;
Academic Journal of Second Military Medical University 2000;0(10):-
Objective: To study the proper medium for tissue culture of the explants from Hypericum perforatum . Methods: The root, stem with node and leaf of the Hypericum perforatum seedling were used as explants, and cultured in basic Murashige and Skoog (MS) medium supplemented with different kinds and different concentrations of hormones. The callus, buds and roots were induced. The cultivation of the tube seedling were carried out. Results: Different hormones and explants had different effects on the induction of callus and the formation of buds. 2,4 D could promote the formation of the callus of Hypericum perforatum . Among cytokinins, 6 BA had great effect on the formation of buds, but the effects of KT and ZT were insignificant. The combination of NAA 1 mg/L and 6 BA 0.2 mg/L was found most effective in promoting the rooting of Hypericum perforatum . Conclusion: The results indicate that the callus is easy to be induced when MS medium supplemented with 2,4 D. The root of explants is most suitable for the rosette bud rapid induction.
4.Establishing better lines and regenerating system of hairy roots of Isatis indigotica with Ri T-DNA
Tiefeng XU ; Hanming ZHANG ; Wei ZHANG ; Ruxian DING ; Bohua LI ;
Academic Journal of Second Military Medical University 2000;0(10):-
Objective: To establish the culture system of hairy root of Isatis indigotica and to induce the regeneration plant of hairy root. Methods: Hairy root of Isatis indigotica was obtained from infected cotyledon explants after infection with Agrobacterium rhizogenes strains A4, R1601 and ATCC15834. The better lines were selected. The growth curve was surveyed and extrinsic factors affecting the growth of hairy roots were investigated. The regeneration plant was induced on MS media with different hormones. The transformation of Ri T DNA was examined through high voltage paper electrophoresis. Results: The hairy root was originally obtained from Isatis indigotica . The regeneration plant was induced on MS media with BA. The result of high voltage paper electrophoresis confirmed the transformation of T DNA from Ri plasmid to the hairy root and regeneration plant. Conclusion: The acquisition of hairy root of Isatis indigotica and regeneration plant of it lay a foundation for the production of active component and introduction of foreign gene. [
5.Genetic transformation of Eclipta prostrata by Ri T-DNA
Hanming ZHANG ; Tiefeng XU ; Ruxian DING ; Bohua LI ;
Chinese Traditional and Herbal Drugs 1994;0(10):-
Object To establish the culture system of hairy root of Eclipta prostrata (L.) L.. Methods Hairy root of E. prostrata was obtained from infected cotyledon explants after infection with Agrobacterium rhizogenes strains A4, R1601 and ATCC15834, elite strains were screened and growth curves determined. The transformation of Ri T DNA was examined through high voltage paper electrophoresis. Results The hairy root was originally obtained from E.prostrata. The result of high voltage paper elctrophoresis confirmed the transformation of T DNA from Ri plasmid to the hairy root. Conclusion The acquisition of hairy root of E. prostrata provided further a foundation for the industrial production of active drug component.
6.Comparative study of three dimensional dynamic contrast-enhanced MR perfusion imaging in healthy volunteers and patients with chronic obstructive pulmonary disease
Yi XIA ; Yu GUAN ; Li FAN ; Shiyuan LIU ; Tiefeng ZHANG ; Bing LI ; Hong YU
Chinese Journal of Radiology 2013;(7):629-633
Objective To prospectively compare MR pulmonary perfusion imaging with quantitative HRCT for the detection of mild chronic obstructive pulmonary disease (COPD) and classification of COPD.Methods Sixty-two consecutive patients with COPD and 17 healthy volunteers underwent pulmonary function test (PFT),HRCT and MR perfusion imaging on the same day.According to the Global Initiative for Chronic Obstructive Lung Disease (GOLD),all COPD patients were classified into 4 stages:stage Ⅰ (n =19),stage Ⅱ (n =17),stage Ⅲ (n =14),stage Ⅳ (n =12).The signal intensity of perfusion defects (SIPD),signal intensity of normal lung perfusion (SInormal) on 3D MR perfusion were obtained through postprocessing and the signal intensity ratio (RSI) was calculated.The total lung volume (TLV) was calculated automatically on HRCT and the total emphysema volume (TEV) was obtained by applying -950 HU thresholds.The TEV/TLV was deduced as emphysema index (EI).Several comparisons were made between the volunteers and COPD patients by one-way ANOVA and Kruskal-Wallis test.Results The RSI,SIPD,PEI,MSI,MSD values of MRI perfusion in volunteers (43.9 ± 7.2,48.2 ± 19.7,31.4,55.7,44.1) were significantly different from those in patients with COPD (18.1 ± 8.1,47.4 ± 20.0,8.6,30.2,22.7) (P < 0.01).The RSI showed a significant difference between stage Ⅰ (24.4 ± 9.8) and stage Ⅲ (15.9 ± 5.3) or Ⅳ (9.2 ± 2.7) and between stage Ⅱ (19.9 ± 3.1) and stage Ⅳ (t =4.05-6.64,P <0.01).However,all MRI perfusion parameters between stage Ⅰ and stage Ⅱ,stage Ⅱ and stage Ⅲ,stage Ⅲ and stage Ⅳ were no differences (t =2.00-4.46,P > 0.05).The median of EI in volunteers and stage Ⅰ-Ⅳ COPD patients were 1.2,3.8,8.0,13.7,18.3,and the quartile range were 3.7,7.1,9.2,10.5,7.7,respectively.The EI in volunteers showed significant differences with that of stage Ⅱ-Ⅳ COPD and the EI of stage Ⅳ was different from that of stage Ⅱ or Ⅰ (t =-7.32--1.85,P < 0.01),but there was no significant difference between volunteers and stage Ⅰ COPD (t =-1.99,P > 0.05).Conclusions The RSI of MRI is more sensitive than that of HRCT for assessing mild COPD.The severity of COPD could be reflected by MRI perfusion and HRCT.
7.Role of performance appraisal management in standardized residents training
Chi CHEN ; Dongjie TIAN ; Tiefeng XU ; Zhongxin ZHAO ; Huan ZHANG ; Lei XIAO ; Jun TAN
Chinese Journal of Medical Education Research 2011;10(12):1492-1494
During the course of standardized residents training,the performance appraisal management of the residents was used besides other regular management methods.The allowances of residents were decided monthly by the evaluation and assessment of many kinds of tests or examinations.Through this new measure,the learning enthusiasm of residents could be fully motivated.The authoritative effectiveness of management was enhanced enormously.Meanwhile,the quality of the educational and training has also been improved greatly.This method could be very useful for the training process.
8.Study on cross-cultural adaptation and health status in medical international students
Li MA ; Tiefeng FU ; Ji QI ; Xiaohong GAO ; Wenli ZHANG ; Xiang LI ; Shuang CAO ; Changyu GAO
Chinese Journal of Medical Education Research 2011;10(11):1379-1382
A descriptive study to investigate the situation of cross-cultural adaptation and health status among medical international students was carried out using cluster sampling method and self-designed questionnaire in Dalian Medical University.148 international students come from 24 countries.There are 5 religions belief among these students.Islam,Catholic,Hindu,Buddhism and Sikhism.The main obstacles of cross-cultural adaptation are language,accommodation and diet.Economic status and stress of interpersonal relationship are also the main causes affecting the physical and mental health of medical international students.
9.Clinicopathological significance of pEZRThr567 expression in lung squamous carcinoma
Yuan ZHANG ; Chenglong JIN ; Shentong WANG ; Yang YANG ; Zhenhua LIN ; Tiefeng JIN
Chinese Journal of Pathophysiology 2015;(5):947-949,955
AIM:To investigate the clinicopathological significance of the protein expression of phosphorylated ezrin at threonine 567 ( pEZRThr567 ) in lung squamous cell carcinoma, adjacent tissues and normal tissues.METHODS:pEZRThr567 protein was detected in lung squamous carcinoma, adjacent and normal tissues by the method of immunohisto-chemistry.The correlation of pEZRThr567 expression with clinicopathological parameters of lung squamous carcinomas was al-so analyzed.The localization of pEZRThr567 was detected by immunofluorescence staining in lung squamous cell line EBC-1. RESULTS:The protein expression of pEZRThr567 in lung squamous carcinoma was significantly higher than that in the adja-cent and normal lung tissues (P<0.01).pEZRThr567 mainly localized on the cell membrane, and its over-expression signi-ficantly correlated with the differentiation, clinical stage and lymph node metastasis in lung squamous carcinoma.CON-CLUSION:pEZRThr567 may be an effective biomarker for prediction of malignant potential and poor prognosis of lung cancer.
10.Analysis of medical research institutions in Shanghai
Hanwen MAO ; Qinlong GU ; Weilin WANG ; Tiefeng XU ; Hong WU ; Jianping WANG ; Kan ZHANG
Chinese Journal of Medical Science Research Management 2010;23(6):370-372,390
This study surveyed medical research institutions in Shanghai for the structure set, input and output, development orientation, bottleneck and other aspects. Problems were found with their relationship management, discipline arrangement, research efficacy and else.