1.Changes of lymphocyte subsets in acute leukemia patients after HLA-mismatched nonmyeloablative hematopoietic stem cell transplantation.
Kai-Xun HU ; Mei GUO ; Chang-Lin YU ; Dan-Hong WANG ; Qi-Yun SUN ; Jian-Hui QIAO ; Guang-Xian LIU ; Tie-Qiang LIU ; Hui-Sheng AI
Journal of Experimental Hematology 2009;17(6):1527-1531
This study was purposed to investigate the reconstitution of immune system in patients with acute lymphocyte leukemia (ALL) or acute myeloid leukemia (AML) after HLA-mismatched nonmyeloablative hematopoietic stem cell transplantation (NHSCT) and its relation with infection and GVHD. 6 ALL and 4 AML patients having HLA-mismatched related donors received the nonmyeloablative precondition regimen composed of fludarabine (Fln), ATG, Ara-C, CTX and total body irradiation (TBI) in dose 2 Gy. The GVHD was prevented and treated by CsA, anti-CD25 antibody and mycophenolic mofetil (MMF) before and after transplantation. The flow cytometry was used to detect the changes of total T cells, help/inducer T cells, suppressor/killer T cells, gamma/delta T cells, B cells, NK cells, NKT cells, regulatory T cells, activated T cells, naive T cells, memory T cells and ratio of CD4/CD8 in patients with remission resulting from chemotherapy before transplantation, and analyse the relation of immunofunctional cells to infection and GVHD after transplantation, compare the difference in recovery of immune system of ALL and AML patients. The results showed that the recovery of total lymphocytes and lymphocyte subsets displayed one's own regular pattern. As compared with patients without GVHD, the counts of lymphocyte subsets in patients with GVHD was higher, while the counts of gamma/delta T cells, regulatory T cells, NK cells, the counts of B cells, NK cells, naive cells and CD4/CD8 ratio as well as the counts of B cells, naive T cells and NK cells were lower at 1 month, 2 - 3 months and 6 - 8 months after transplantation respectively. The total T cells and subsets recovered slowly, but NK cells and NKT cells recovered rapidly in patients with infection at early period after transplantation, the B cells and naive B cells recovered rapidly at 3 months after transplantation. There was no difference in lymphocyte recovery between ALL and AML patients. It is concluded that the analysis of each lymphocyte subsets may indirectly show the recovery of thymus function in patients, the changes of NK cells, B cells and naive T cells have an important significance for identifying and forecasting the GVHD and infection.
Adolescent
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Adult
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Child
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Female
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Graft vs Host Disease
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etiology
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HLA Antigens
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immunology
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Hematopoietic Stem Cell Transplantation
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Humans
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Leukemia, Myeloid, Acute
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immunology
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Lymphocyte Subsets
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immunology
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Male
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Middle Aged
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Postoperative Period
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Precursor Cell Lymphoblastic Leukemia-Lymphoma
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immunology
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Young Adult
4.The molecular mechanisms of As_2O_3- induced un-terminal differentiation of HL-60 cells
hou-qiao, BAI ; tie-jun, GUO ; pei-e, WEN ; hai-quan, REN ; yu-kun, ZHANG ; tian-hua, TANG ; hao, ZHANG ; gao-feng, BI ; jia-ning, LIU ; guo-sheng, JIANG
Journal of International Oncology 2006;0(05):-
To approach the effect of CCAAT/enhancer binding proteins (C/EBPs) on un-terminal differentiation of HL-60 cells after treatment with Arsenic Trioxide ( As_2O_3) . Methods The changes of cell morphology were observed by Wright staining, the alteration in the cell proliferation was determined by WST1 experiment and the NBT reduction assay was used to detect the differentiation condition of cells, determination and analysis cell cycle. The expressions of C/EBP? and C/EBP? mRNA in HL-60 cells exposed to ATRA and As_2O_3 were assayed by semi-quantitative RT-PCR. Results It was found that ATRA could up- regulate the mRNA expression of C/EBP? obviously, but down-regulate the mRNA expression of C/EBP?. As_2O_3 could up-regulate the mRNA expression of C/EBP? lightly, down-regulate the expression of C/EBP?. Conclusion Both of ATRA and As_2O_3 can down-regulate the mRNA expression of C/EBP?,but there is no significant difference between these two groups,ATRA and As2O3 can up- regulate the mRNA expression of C/EBP?, with significant differences (P
5.Application of HLA-A*0201/WT1 pentamer combined with intracellular IFNgamma+ staining in detecting circulating WT1 specific T cells in leukemia.
Li WEI ; Xue-Dong SUN ; Hong-Li ZUO ; Tie-Qiang LIU ; Mei GUO ; Guang-Xian LIU ; Qi-Yun SUN ; Jian-Hui QIAO ; Dan-Hong WANG ; Chang-Lin YU ; Kai-Xun HU ; Zheng DONG ; Hui-Sheng AI
Journal of Experimental Hematology 2010;18(2):505-509
This study was purposed to investigate the value of combination of pentamer and intracellular IFNgamma staining in the qualitative and quantitative detection of circulating antigen-specific T cells. WT1 expressions in 14 HLA-A*0201+ patients and their matched donors were detected by RT-PCR, and circulating WT1 specific T cells were assayed by HLA-A*0201/WT1 pentamer combined with intracellular IFNgamma+ staining. The results showed that the low level of WT1 expression was found only in 2 cases out of 14 donors, but different levels of WT1 expression could be observed in all leukemic patients. The WT1+CD8+ CTL and WT1+IFNgamma+ cells did not detected in all 14 donors, but WT1+CD8+ CTL cells in 2 patients and WT1+IFNgamma+ cells in 3 patients could be detected before transplantation respectively, there was no significant difference between them, while the WT1+CD8+ CTL cells and WT1+IFNgamma+ cells both could be detected in all 14 patients after transplantation, the positive detection rate after transplantation was obviously higher than that before transplantation. The WT1+CD8+ and WT1+ IFNgamma+ cells could be detected within 30 days after transplantation, but the positive detection rate of WT1+IFNgamma+ cells was higher than that of WT1+CD8+ CTL cells (p=0.014). The median peak value of WT1+CD8+ CTL cells was 0.18% in 14 patients, and the median peak value of WT1+IFNgamma+ cells was 0.83% in 14 patients, the later was significantly higher than former. The median peak time of WT1+CD8+ CTL cells was 75 days after transplantation, while the WT1+IFNgamma+ cells was 105 days after transplantation, there was no significant difference between them. It is concluded that pentamer and intracellular IFNgamma staining may effectively detect circulating WT1 specific T cells in leukemic patients, and the combination of these two methods profit to the exact qualitation and quantitation of circulating antigen-specific T cells.
Adolescent
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Adult
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Child
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Female
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Flow Cytometry
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HLA-A Antigens
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analysis
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HLA-A2 Antigen
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Humans
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Interferon-gamma
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analysis
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Leukemia
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blood
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genetics
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immunology
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Male
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Middle Aged
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Staining and Labeling
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T-Lymphocytes, Cytotoxic
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immunology
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metabolism
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WT1 Proteins
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genetics
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immunology
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metabolism
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Young Adult
6.Relationship between WT1-specific T-cell subsets and graft-versus-host disease after nonmyeloablative allogeneic transplantation.
Li WEI ; Hong-li ZUO ; Tie-qiang LIU ; Xue-dong SUN ; Mei GUO ; Guang-xian LIU ; Qi-yun SUN ; Jian-hui QIAO ; Dan-hong WANG ; Chang-lin YU ; Kai-xun HU ; Zheng DONG ; Hui-sheng AI
Chinese Journal of Hematology 2011;32(2):89-93
OBJECTIVETo explore the relationship between WT1-induced T-cell subsets and graft-versus-host disease (GVHD) after nonmyeloablative allogeneic hematopoietic stem cell transplantation (NST).
METHODSPeripheral blood mononucleated cells (PBMCs) from 19 patients who expressed WT1 and developed GVHD after NST were simulated by WT1126-134 peptide in vitro, and proportions of WT1-induced-T-cell subsets (Tc1, Tc2, Th1, Th2 cells) before and after transplant were detected by intracellular cytokine staining (ICCS) assay. WT1-specific CD8(+) CTLs of 14 patients with HLA-A*0201 were detected by HLA-A*0201/WT1 pentamer.
RESULTS(1) 17 of 19 patients developed GVHD, among whom proportions of Tc1 and Th1 cells, achieved peak value in 16 patients at occurrence of GVHD (P = 0.039); (2) The peak proportions of Tc1 and Th1 cells in patients with aGVHD above grade II were higher than those with grade I, but being no statistical difference (P = 0.900 and P = 0.140, respectively); (3) The peak proportion of Th1 cells (P = 0.004), but not Tc1 cells (P = 0.060) in patients with extensive cGVHD was significantly higher than that in patients with limited one; (4) Proportions of Tc1, Th1 and WT1(+)CD8(+)CTL in patients without GVHD were similar to those in patients with Grade I aGVHD, but lower than those in aGVHD above grade II.
CONCLUSIONGVHD promotes the generation of WT1-induced GVL effect, and the intensity of the latter maybe correlated with the intensity of GVHD, especially cGVHD. Th1 cells play a more important role in the enhancement of WT1-induced GVL effect in extensive cGVHD patient than in limited cGVHD patients.
Adolescent ; Adult ; Female ; Graft vs Host Disease ; etiology ; Graft vs Leukemia Effect ; Hematopoietic Stem Cell Transplantation ; adverse effects ; Humans ; Male ; Middle Aged ; T-Lymphocyte Subsets ; immunology ; Transplantation, Homologous ; WT1 Proteins ; metabolism ; Young Adult
7.Efficacy and safety of Tripterygium wilfordii hook F versus acitretin in moderate to severe psoriasis vulgaris: a randomized clinical trial.
Chao WU ; Hong-Zhong JIN ; Dan SHU ; Feng LI ; Chun-Xia HE ; Ju QIAO ; Xiao-Ling YU ; Ying ZHANG ; Yi-Bo HE ; Tie-Jun LIU
Chinese Medical Journal 2015;128(4):443-449
BACKGROUNDFew clinical trials have evaluated the efficacy and safety of Tripterygium wilfordii Hook F (TwHF) compared with acitretin in psoriasis. We aimed to compare the efficacy and safety of TwHF compared with acitretin in the treatment of moderate to severe psoriasis vulgaris.
METHODSAdults with Psoriasis Area Severity Index (PASI) score ≥ 10 and psoriasis-affected body surface area ≥ 10% were randomized into a TwHF (20 mg, 3 times a day) or acitretin group (30 mg, once a day). The treatment course lasted for 8 weeks. Patients were assessed at baseline and at 2, 4, and 8 weeks. Laboratory tests were performed at baseline, week 4, and week 8. The data were analyzed using paired samples t-test or analysis of variance (ANOVA).
RESULTSA total of 115 patients was enrolled (58 TwHF; 57 acitretin). The median PASI score improved in the TwHF group by 50.4% and in the acitretin group by 42.7%. There was no significant difference in median PASI improvement between two groups at 2, 4, and 8 weeks. There was also no significant difference in PASI 25, PASI 50, PASI 75, and PASI 90 response between the two groups at 2, 4, and 8 weeks. There was a significant increase in the level of aspartate transaminase and triglycerides in the TwHF group (P = 0.026 and P = 0.011, respectively). In the acitretin group, there was a significant increase in the level of alanine transaminase, cholesterol, and high-density lipoprotein (P = 0.030, P < 0.01, and P < 0.01, respectively).
CONCLUSIONSThere was no significant difference in treatment efficacy between the TwHF and acitretin groups within 8 weeks, but there were fewer treatment-related adverse events in the TwHF group.
Acitretin ; therapeutic use ; Adolescent ; Adult ; Aged ; Female ; Humans ; Male ; Middle Aged ; Plant Extracts ; therapeutic use ; Psoriasis ; drug therapy ; Treatment Outcome ; Tripterygium ; chemistry ; Young Adult
8.Identification,biological characteristics and fungicide screening of pathogen of southern blight in Cynanchum stauntonii.
Jin-Xin LI ; Qiao-Huan CHEN ; Yu-Huan MIAO ; Tie-Lin WANG ; Da-Hui LIU
China Journal of Chinese Materia Medica 2021;46(13):3303-3310
During the high-temperature and rainy season from June to October in 2017-2019,serious southern blight broke out in the Cynanchum stauntonii planting area in Tuanfeng county,Hubei province,which had a great impact on the yield and quality of medicinal materials. In this study,the pathogen of C. stauntonii was isolated,purified,and identified,and the pathogenicity was tested according to Koch's postulates. Meanwhile,the biological characteristics of the pathogen were analyzed. On this basis,the effective fungicides were screened in laboratory. Finally,the pathogen( BQ-1) was identified as Athelia rolfsii( Deuteromycotina,Basidiomycota,anamorph: Sclerotium rolfsii). The optimum growth conditions for BQ-1 were 25-30 ℃,p H 5-8,and alternating light and dark.The effective chemical fungicides were lime-sulphur-synthelic-solution( LSSS) and flusilazole,and the effective botanical fungicide was osthole. BQ-1 was highly homologous to the pathogen HS-1 of peanut southern blight,with the similarity of 18 S r DNA and TEF sequences at 99. 09%. The southern blight in C. stauntonii might be resulted from that in peanut. In the production of C. stauntonii,the following measures should be taken: avoiding rotation or neighboring with peanut,draining water from June to October to reduce humidity,and reasonably applying fungicides.
Basidiomycota
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Cynanchum
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Fungicides, Industrial/pharmacology*
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Humidity
9.Fusarium Wilt Changes Microbial Community Structure in Rhizosphere Soil of Chrysanthemum morifolium
Qiao-huan CHEN ; Yu-huan MIAO ; Tie-lin WANG ; Lan-ping GUO ; Da-hui LIU
Chinese Journal of Experimental Traditional Medical Formulae 2021;27(11):180-186
Objective:To explore the differences in rhizosphere microbial community structure between
10.The mid-term results of the staged total aortic replacement in Stanford type A aortic dissection
Jianmao HONG ; Yipeng GE ; Lijian CHENG ; Haiou HU ; Ruidong QI ; Zhiyu QIAO ; Chengnan LI ; Tie ZHENG ; Lei CHEN ; Jun ZHENG ; Yongmin LIU ; Junming ZHU ; Lizhong SUN
Chinese Journal of Thoracic and Cardiovascular Surgery 2018;34(4):196-199
Objective To explore the mid-term results of the staged total aortic replacement in Stanford type A aortic dissection.Methods During March 2009 to September 2016,a total of 49 patients with Stanford type A aortic dissection in Beijing Anzhen Hospital cardiovascular center underwent total aortic replacement with a median age of 36 (27,41 years),male 36 (73.5%) cases.30 (61.2%) cases of them combined with Marfan syndrome.Results The interval between two stage operation was 23 (10,57) months.In the first stage operation,45 (91.8%) cases underwent Sun's procedure,2 (4.1%) underwent total aortic arch replacement,2 (4.1%) cases underwent classic elephant trunk and total aortic arch replacement.All patients underwent thoracoabdominal aortic repair(TAAAR).Deep hypothermic circulatory arrest surgery was 12(24.5%)cases in the second stage.7 (14.3%)cases dead postoperative.Spinal cord related complications happened in 3 (6.1%) cases with stroke in 2(4.1%) cases,acute renal insufficiency in 7(14.3 %) cases,respiratory insufficiency in 7 cases (14.3%),re-operation for hemostasis in 3 (6.1%) cases and gastrointestinal bleeding in 3 cases(6.1%).Univariate analysis showed that the interval between two stage operation,operation time,deep hypothermic circulatory arrest surgery are risk factors for in-hospital mortality;multivariate analysis showed that deep hypothermic circulatory arrest surgery and the interval between two stage operation were independent risk factors for in-hospital mortality.3 years,5 years survival rate were 94.4% and 78.7%.Conclusion For Stanford type A dissection especially the thoracoabdominal aortic expands,staged total aortic replacement shows good mid-term results.Block stentgraft can reduce the use of deep hypothermic circulatory arrests to decrease the mortality.