2.The research of the foamy fracture fixation splint.
Li-Ming TANG ; Tie-Bing LIU ; Li-Sheng ZHANG ; Sheng-Li ZHENG ; Sheng-Jun LIU ; Zheng WANG
Chinese Journal of Medical Instrumentation 2009;33(5):354-355
A new production method of fracture fixation splint is introduced in the paper. The basic raw materials are multi-isocyanic acid, mixed with surfactants, catalysts, fillers, and so on. The splint, with light weight, high strength, and good injured anastomosis site, could be able to shape easily. It is dry and comfortable, avoiding a gas-tight uncomfortable feeling, easy to remove, remodel, nurse, clean and disinfect after fixed. Patients also could film review directly with fixed splint.
Equipment Design
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External Fixators
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Fracture Fixation
3.Study of three-dimensional model on biomechanical characteristics of mandibular fractures in different site.
Chun-li LIU ; An-ping WU ; Zheng-guo PIAO ; Hong WU ; Tie-nan PENG ; Lei LIU
West China Journal of Stomatology 2007;25(4):320-322
OBJECTIVETo determine the biomechanical characteristics of mandibular fractures in different site.
METHODSNine adult mandibular specimens were measured precisely. The data was used to establish a three-dimensional model. When mandibular was under functional loading, the bending and torsion moment as well as shear force of angle, body and symphyseal fracture was calculated. The data were analyzed by Origin 6.0 software.
RESULTSAngle fracture had relatively high positive bending moment and high shear force. Body fracture had positive as well as negative bending moment and the highest torsion moments. Symphyseal fracture had only negative bending moment and relatively low shear force.
CONCLUSIONAngle, body and symphyseal fractures each have a biomechanics characteristic. These biomechanics characteristic should have an important meaning in the treatment of mandibular fractures and instructing patient how to bite correctly.
Adult ; Biomechanical Phenomena ; Humans ; Mandible ; Mandibular Fractures
4.Detection of MSH2 gene mutation by PCR.
Duo ZHENG ; Xiao-ping LIU ; Tie-gang LI ; Jun LI ; Li-jun TANG ; Wei-xin HU
Journal of Central South University(Medical Sciences) 2006;31(2):200-203
OBJECTIVE:
To establish a genetic diagnosis method for a novel MSH2 mutation.
METHODS:
A specific primer on the mutated site of MSH2 was synthesized and PCR was conducted using the specific primer and another downstream primer. PCR products were electrophoresed and then the carriers with the novel gene mutation of the carriers or non-carriers were identified.
RESULTS:
MSH2 in a hereditary nonpolyposis colorectal cancer family were successfully found.
CONCLUSION
The method is effective and simple for genetic diagnosis of the novel mutation in MSH2.
Base Sequence
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Colorectal Neoplasms, Hereditary Nonpolyposis
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genetics
;
DNA Mutational Analysis
;
methods
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Female
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Humans
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Male
;
Molecular Sequence Data
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MutS Homolog 2 Protein
;
genetics
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Pedigree
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Point Mutation
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Polymerase Chain Reaction
5.Comparison of the status of STAT4 tyrosine phosphorylation in peripheral T-lymphocytes induced by IL-12 in rheumatoid arthritis and osteoarthritis.
Zhen-Jie SUN ; Yu-Jun ZHANG ; Tie-Zheng SUN ; Ao XIONG ; Rui-Bo LIU ; Hou-Shan LÜ
China Journal of Orthopaedics and Traumatology 2011;24(4):295-298
OBJECTIVETo compare the levels of STAT4 tyrosine phosphorylation in peripheral T-lymphocytes induced by IL-12 in rheumatoid arthritis (RA) and osteoarthritis (OA).
METHODSFrom May 2007 to August 2009, peripheral blood mononuclear cells (PBMCs) were isolated from RA patients [RA group, all the cases were female, the age was from 28 to 55 years with an average of (45.0 +/- 13.0) years] and OA patients [OA group, all the cases also were female; the age was from 55 to 75 years with an average of (67.0 +/- 9.6) years]. The purity of T-lymphocytes from PBMCs was accredited by flow cytometry. The IL-12 of 50 ng/ml added in T-lymphocytes, the levels of STAT4 tyrosine phosphorylation were detected by western blot after different time intervals (0, 10, 30, 60 min).
RESULTSThe purity of T-lymphocytes were above 91% through diremption and depuration for peripheral blood monouclear cells. The levels of STAT4 tyrosine phosphorylation in T-lymphocytes from RA induced by IL-12 were higher than that from OA in the different times (10, 30, 60 min); after 30 min, its levels from RA and OA achieved to crest value.
CONCLUSIONSTAT4 in peripheral T-lymphocytes of rheumatoid arthritis was more easily to be activated than osteoarthritis.
Adult ; Aged ; Arthritis, Rheumatoid ; immunology ; Female ; Humans ; Interleukin-12 ; pharmacology ; Middle Aged ; Osteoarthritis ; immunology ; Phosphorylation ; Polymorphism, Single Nucleotide ; STAT4 Transcription Factor ; genetics ; metabolism ; T-Lymphocytes ; drug effects ; metabolism ; Tyrosine ; metabolism
6.Effects of varglaucocalyx on c-fos gene expression during global myocardial ischemia-reperfusion in rat.
Lan-di LIU ; Li-ka YE ; Dong-jun PAN ; Yan-duo JIANG ; Tie-zheng ZHANG ; Hui YANG
China Journal of Chinese Materia Medica 2003;28(4):358-361
OBJECTIVETo determine the effects of Varglaucocalyx on c-fos gene expression during global myocardial ischemia-reperfusion.
METHODForty Wistar rats were divided into 5 groups: group N as control; group CN as ischemia-reperfusion control and group XH, XM and XL treated with Varglaucocalyx 5%, 1%, 0.5% respectively prior to ischemia-reperfusion. The isolated rat hearts were perfused in condition of constant temperature and pressure, and then the left ventricular myocardiums were extracted for use. The expression of c-fos protein was detected by immunochemical method. The expression of c-fos protein were quantified by using computer image analysis system.
RESULTCompared with the values of group N, protein expressions relative area of c-fos gene (PERA) were increased significantly in group CN, XH, XM, XL(P < 0.01), but decreased significantly in group XH, XM, XL, compared with those of group CN (P < 0.05). The PERA of c-fos gene in group XM, XL were significantly lower than in group XH (P < 0.01), and the PERA of c-fos gene in group XM were lower than in group XL(P < 0.05).
CONCLUSIONVarglaucocalyx can effectively depress the expression of c-fos gene in myocardium which may account for its protection against myocardial ischemia-reperfusion injury, and the middle and the low concentrations of Varglaucocalyx are more effective than the high concentrations.
Animals ; Cardiotonic Agents ; pharmacology ; Drugs, Chinese Herbal ; isolation & purification ; pharmacology ; Female ; Gene Expression Regulation ; drug effects ; Genes, fos ; Isodon ; chemistry ; Male ; Myocardial Reperfusion Injury ; etiology ; metabolism ; Myocardium ; metabolism ; Plants, Medicinal ; chemistry ; Proto-Oncogene Proteins c-fos ; biosynthesis ; Random Allocation ; Rats ; Rats, Wistar
7.Expression of acidophilic alpha-amylase from Alicyclobacillus acidocaldarius.
Tie-Zheng YUAN ; Bin YAO ; Hui-Ying LUO ; Ya-Ru WANG ; Ning-Feng WU ; Yun-Liu FAN
Chinese Journal of Biotechnology 2005;21(1):78-83
The alpha-amylase (EC 3.2.1.1) from the Gram-positive Alicyclobacillus acidocaldarius was one kind of thermoacidophilic enzyme, with optimal temperature and pH of 75 degrees C and 3, respectively. The nucleotide sequence of the gene amy was cloned by PCR. The gene amy was 3901bp long, comprising one open reading frame encoding a polypeptide of 1301 amino acids. The calculated molecular weight of the alpha-amylase AMY was about 140kD. The gene amy was expressed in E. coli BL21 (DE3) and Pichia pastoris respectively, and both of the cloned proteins had bioactivity. The activity of amylase expressed in P. pastoris was further testified by amylase activity staining. The alpha-amylase expressed in P. pastoris had been purified and characterized. The apparent molecular weight of that was about 160kD according to SDS-PAGE. The optimum of pH for the enzyme was pH 3.2 as the native enzyme was; but the optimum of temperature was 65 degrees C and a little lower than that of the native enzyme. Above 50% of relative activity remained after incubation for 30 minutes in 70 degrees C. So the enzyme expressed by P. pastoris was also thermoacidophilic. Moreover some sequence was cloned by PCR, which ranged from + 1174 bp to + 3288 bp in the gene amy, encoding 705 amino acids with the calculated molecular weight of 79kD. The truncated gene amy' was expressed in E. coli BL21 (DE3) induced by 1 mmol/L IPTG, and the expressed enzyme also retained alpha-amylase activity.
Bacillus
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enzymology
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genetics
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Bacterial Proteins
;
genetics
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isolation & purification
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metabolism
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Cloning, Molecular
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Escherichia coli
;
genetics
;
metabolism
;
Genetic Vectors
;
genetics
;
Pichia
;
genetics
;
metabolism
;
alpha-Amylases
;
genetics
;
isolation & purification
;
metabolism
8.Toxicity of acrylamide on male reproduction.
Hong-Xiu SONG ; Ran WANG ; Shao-Xian CAO ; Tie-Zheng LIU
National Journal of Andrology 2008;14(2):159-162
Acrylamide is a common chemical material, extensively used in industry and scientific experiments. Recently, it has been reported that starchy food cooked at high temperature can produce acrylamide. Acrylamide monomer has several toxic effects and the extensive concern for its toxicity has arisen with the finding of acrylamide formation in some processed foods. Researches have shown that acrylamide monomer can cause reproductive toxicity, including toxic effects on male reproductive behavior, male reproductive endocrine function and spermatogenesis. The mechanisms may include the effects of acrylamide on Leydig cells, the formation of motor protein/ chromosomal/DNA alkylation and damage by oxidative stress.
Acrylamide
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toxicity
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Animals
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Genitalia, Male
;
drug effects
;
physiology
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Male
;
Sexual Behavior, Animal
;
drug effects
;
physiology
;
Spermatogenesis
;
drug effects
9.Phytochemical and pharmacological research progress in Tussilago farfara.
Ke-yue LIU ; Tie-jun ZHANG ; Wen-yuan GAO ; Hai-xia CHEN ; Yi-nan ZHENG
China Journal of Chinese Materia Medica 2006;31(22):1837-1841
Tussilago farfara contained the chemical constitutents including terpenes, flavonoids, and alkanoids. It has been used for the relief of coughs and as an expectorant, blood pressure raiser, platelet activating factor inhibitor and anti-inflammatory agents. This paper reviewed the phytochemical and pharmacological research progress in T. farfara, including the chemical ingredients, the pharmaceutical activities and the security evaluation aiming at its toxicity. The problems at present and the reseach direction for the future on T. farfara have been put forward.
Alkaloids
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chemistry
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pharmacology
;
toxicity
;
Animals
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Drug Evaluation, Preclinical
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methods
;
trends
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Drugs, Chinese Herbal
;
isolation & purification
;
pharmacology
;
toxicity
;
Flavonoids
;
chemistry
;
pharmacology
;
toxicity
;
Molecular Structure
;
Plants, Medicinal
;
chemistry
;
Terpenes
;
chemistry
;
pharmacology
;
toxicity
;
Tussilago
;
chemistry
10.Surgical repair of left-sided cervical aortic arch aneurysm
Tie ZHENG ; Yongliang ZHONG ; Ruidong QI ; Lijian CHENG ; Yipeng GE ; Lei CHEN ; Wei LIU ; Chengnan LI ; Xiaoyan XING ; Junming ZHU ; Lizhong SUN
Chinese Journal of Thoracic and Cardiovascular Surgery 2016;32(7):391-394
Objective To summarize our experience of surgical repair for cervical aortic arch(CAA) aneurysm and eval-uate early and midterm results of these patients.Methods From January 2010 to December 2014, 22 patients with left-sided CAA aneurysm admitted in our center.There were 6 male and 16 female patients with a mean age of(34.09 ±13.14) years. Comorbidities included pseudocoarctation in 9 patients, hypertension in 4 patients, and aortic valve insufficiency, Stanford type B aortic dissection and middle cerebral artery aneurysm each had 1 patient.All of the patients underwent surgical aortic arch re-construction using artificial graft replacement.Among them, 4(4/22, 18.18%) were performed under moderate hypothermic circulatory arrest(MHCA) combined with selective antegrade cerebral perfusion(SACP) via a median sternotomy, and concom-itant aortic valve replacement(AVR) was implemented in 1 patient.18(18/22, 81.82%) were performed via posterolateral left thoracotomy through the 4th intercostal space, and adjunct methods applied included partial CPB and “simple clamping” in 10 and 8 of these patients respectively.Results The average mechanical ventilation time and ICU stay time was (13.05 ± 4.73)h and(19.14 ±8.08) h respectively.1 patient required repeat thoracotomy for bleeding, 1 patient with delayed wound healing and 1 patient suffered transient liver dysfunction.There were no in-hospital deaths.Mean follow-up time was 34.73 months, and 3 patients were lost during follow-up.There were no late deaths during follow-up.Conclusion Repair of CAA is indicated for the patients with arch aneurysm formation .According to the locations and types of aneurysms and other concomi-tant proximal cardiovascular diseases, performing one-stage surgical aortic arch reconstruction with individualized incisions , ad-junct methods and operative procedures can obtain satisfactory clinical outcomes in patients with CAA aneurysm .