Objective:To investigate the relationship between meaning in life and procrastination of college students, and whether time value plays an mediating role between them.Methods:A total of 605 college students in Guizhou completed general procrastination scale, time value scale and life meaning scale.SPSS 22.0 software and PROCESS maro program were used for descriptive statistics, correlation analysis and mediating effect exploration.Results:(1) The procrastination score of college students was (34.40±7.74), the score of time value was (33.17±4.41), and the score of meaning in life was(46.03±8.81). (2) Procrastination was negatively correlated with meaning in life and time value ( r=-0.38, -0.16 respectively, P<0.01). While meaning in life was positively correlated with time value( r=0.31, P<0.01). (3) The mediating effect of time value perception between meaning seeking and procrastination was 22.6%.The direct effect of meaning in life on procrastination was 77.4%. Conclusion:College students with high meaning seeking is less procrastination, and time value mediates the effect between procrastination and meaning in life.Improving college students' meaning in life is conducive to indirectly reducing procrastination through time value.

Objective　To establish a dual droplet digital PCR (ddPCR) assay for herpes simplex virus type I (HSV-1) and varicella-zoster virus (VZV). Methods The specific primers and probes were derived based on the conserved regions of HSV-1 and VZV genome. The primer-probe combinations were screened, and the annealing temperatures and primer-probe concentration ratios of the dual-droplet digital PCR reaction were optimized to establish a dual-droplet digital PCR reaction system for HSV-1 and VZV, which was tested for other viruses and validated for clinical samples. The sensitivity, specificity, and reproducibility of the established dual microtiter digital PCR method were analyzed. Results The optimal concentrations of primers and probes for the dual ddPCR detection method of HSV-I and VZV were determined to be 800 nmol/L and 250 nmol/L, respectively, with an optimal annealing temperature of 56 ℃. The correlation coefficient (R2) of the standard curve of the dual ddPCR assay was 0.99, showing a clear linear relationship. The method showed high sensitivity, with the lowest detection limit of herpes simplex virus type I being 2.97 copies/μL, and for VZV being 2.73 copies/μL. The repeatability was high with a small coefficient of variation and stable detection results; the specificity was excellent, and no cross-reaction was found with herpes simplex virus type Ⅱ, Epstein-Barr virus, Adenovirus, Coxsackievirus (CA6/CA10/CA16), Cytomegalovirus, Human Cytomegalovirus, Human enterovirus 71, Japanese Encephalitis virus, West Nile virus, Measles virus, Mumps virus, and human nucleic acids. Conclusions The dual droplet digital PCR assay for herpes simplex virus type I and varicella-zoster virus established in this experiment has strong sensitivity, specificity, and high repeatability, and can provide a solution for rapid quantitative detection of the two viruses in different scenarios.