Objective:To investigate the effect of “Internet+Healthcare” combined with traditional education on the quality of bowel preparation for colonoscopy.Methods:A double-blind randomized parallel controlled trial was conducted. A total of 660 patients who underwent colonoscopy from August 2020 to August 2021 were enrolled. The patients were randomly assigned to receive “Internet+Healthcare” education combined with traditional education (study group, n=330 cases) or the traditional education alone (control group, n=330).The Boston bowel preparation scale, the qualified rate of bowel preparation and the adenoma detection rate of the two groups were compared. The factors affecting bowel preparation were determined by multivariate logistic regression analysis. Results:The Boston bowel preparation scale (6.1±1.1 vs. 5.3±1.2, t=-9.18, P<0.001), the qualified rate of bowel preparation [93.5% (300/321) vs. 75.8% (244/322), &chi;2=38.58, P<0.001], and the adenoma detection rate [39.3%(126/321) vs. 30.4%(98/322), &chi;2=5.51, P=0.019] in the study group were significantly higher than those in the control group. The factors related to qualified bowel preparation were female gender ( OR=2.19, 95 %CI:1.33-3.59, P=0.002), no hypertension ( OR=1.91, 95 %CI:1.17-3.12, P=0.010), diarrhea ( OR=4.84, 95 %CI:1.22-19.24, P=0.025), and “Internet+Healthcare” combined with traditional education ( OR=4.79, 95 %CI:2.81-8.16, P<0.001).The factors related to unqualified bowel preparation were no colonoscopy history ( OR=0.34, 95 %CI:0.20-0.56, P<0.001) and no diabetes ( OR=0.44, 95 %CI:0.24-0.80, P=0.008). Conclusions:Compared with traditional education, patients who received “Internet+Healthcare” education combined with traditional education have high quality of bowel preparation and high adenoma detection rate in colonoscopy. To obtain qualified bowel preparation for colonoscopy attention should be paid to factors affecting bowel preparation quality.

Objective:To apply the maker teaching method in the teaching of gastroenterology to expand students' thinking and stimulate their innovative consciousness, so as to obtain better teaching effects and improve students' achievements, degrees of teaching satisfaction and learning interest.Methods:All the students of two long-schooling classes who were on clinical probation in Xuanwu Hospital of Capital Medical University from January 2020 to September 2021 were selected as the research objects. All the students in one class were classified as the experimental group (SCS maker teaching group), with 55 students in total; the other class students were classified as the control group (traditional teaching group), with 56 students in total. Before teaching, students in the two groups were given a mapping test and scored by the Williams creativity tendency scale, and after teaching, students were given a closed-book test and scored by the Williams creativity tendency scale. Finally, the students' satisfaction score and study interest were investigated by questionnaire. SPSS 23.0 was used to perform t-test and Chi-square test for the data. Results:The experimental group's post-teaching test scores, total theoretical scores (68.11±9.27 vs. 58.66±6.55, t=-6.19, P<0.001), choice questions (21.85±3.94 vs. 18.96±4.59, t=-3.56, P=0.001), short answer questions (13.55±3.67 vs. 11.18±3.23, t=-3.61, P <0.001), simple case analysis (12.67±3.82 vs. 11.14±2.82, t=-2.40, P=0.018), complex case analysis (20.04±5.62 vs. 17.38±5.30, t=-2.57, P=0.012) and film reading tests (36.53±7.59 vs. 33.14±7.82, t=-2.31, P=0.023) were all higher than those of the control group, and the differences were statistically significant. The scores of the Williams creativity tendency scale (98.91±9.81 vs. 91.09±10.60, t=-4.03, P <0.001), satisfaction scores (6.76±1.78 vs. 5.66±1.91, t=-3.14, P=0.002) and learning interest scores (6.49±2.00 vs. 5.41±1.82, t=-2.98, P=0.004) of the experimental group were higher than those of control group, and the differences were statistically significant. Conclusion:The application of maker teaching method in the teaching of gastroenterology can cultivate students' innovative thinking, improve medical students' understanding of digestive endoscopy, induce their desire to learn digestive endoscopy, and improve students' satisfaction with teaching.

AIM:To investigate the role of specific long noncoding RNA SLC25a6(lncSLC25a6)in homocys-teine(Hcy)-induced cuproptosis in cardiomyocytes.METHODS:Rat cardiomyocytes were cultured in vitro and divided into control group and Hcy group.After 48 h of intervention,the expression levels of cuproptosis-related proteins,ferre-doxin 1(FDX1)and heat shock protein 70(HSP70),were detected by Western blot and immunofluorescence staining.The oxidative stress state of cardiomyocytes was assessed using fluorescence staining,and the intracellular Cu2+levels were measured using a copper ion assay kit.Furthermore,the impact of Hcy on the expression of cuproptosis-related proteins in cardiomyocytes was analyzed following overexpression of lncSLC25a6.RESULTS:Compared with the control group,80 μmol/L Hcy significantly accelerated cardiomyocyte damage,with a notable underexpression of lncSLC25a6(P<0.05).Western blot results indicated that,compared with the control group,the expression level of FDX1 in the Hcy intervention group was significantly reduced(P<0.05),while the expression level of HSP70 was significantly elevated(P<0.05),and the expression level of copper ions in cardiomyocytes of the Hcy group was increased(P<0.05).Immunofluorescence staining showed a significant reduction in FDX1 fluorescence intensity and a significant increase in HSP70 fluorescence in-tensity in the Hcy group.Further overexpression of lncSLC25a6 significantly mitigated Hcy-induced cuproptosis in cardio-myocytes,resulting in elevated expression of FDX1 and reduced expression of HSP70(P<0.05).Pearson correlation analysis demonstrated that the expression level of lncSLC25a6 was negatively correlated with FDX1 protein expression(r=-0.676,P=0.046)and positively correlated with HSP70 expression(r=0.680,P=0.044).CONCLUSION:lnc-SLC25a6 significantly mitigates Hcy-induced cuproptosis in cardiomyocytes,positioning it as a potential therapeutic target for managing Hcy-induced cardiac injury.

Objective To study the role of ubiquitin-conjugating enzyme 9(UBC9)in the pyroptosis of homocysteine-induced macrophages mediated by small ubiquitin-like modifier(SUMO)modification.Methods First,the effects of homocysteine at different concentrations(0 μmol/L,50 μ.mol/L,100 μmol/L,150 μmol/L and 200 μmol/L)on the viability and pyrodeath of mouse macrophages(RAW264.7)were detected by CCK-8 and Western blot.Western blot was used to detect the expression levels of UBC9,SUMO-1,and the inflammatory cytokine IL-1β in different groups of cells.qRT-PCR was used to detect the mRNA expression of UBC9 before and after RNA interference and the expression of UBC9,pyrogen-related protein,and SUMO-1 after RNA interference.Results After stimulation with 100 μmol/L homocysteine,the effect of macrophage activity was minimal,and NLRP3 and Caspase-1 were the proteins with the most obvious increase in expression(P＜0.05).Compared with the Control group,the Hcy group's expression of IL-1β and SUMO-1 was increased(P＜0.01).Compared with the Control group,the Hcy group's UBC9 protein and mRNA levels were increased(P＜0.05).The expression of NLRP3,Caspase-1,IL-1β,UBC9,and SUMO-1 was decreased in the si-UBC9+Hcy group compared with the si-NC+Hcy group(P＜0.01).Conclusions Homocysteine induces pyroptosis in macrophages,and its mechanism of action is related to the up-regulation of UBC9 to induce SUMO modification.