Objective: To investigate the biological function of soybean selenoprotein by the study of anti-oxidation,immunological and anticarcinogenic efficacy. Method: The soybean selenoprotein at different doses of Se were fed intra-gastrically in Kunming mice. A low Se feedstuff was used as basic diet. Result: (1) when dose is below 202.5 ?g/kg bw Se, the number of white cell and red cell was increased remarkably, and the activity of GSH-Px and SOD in liver and serum was increased at dose dependent manner, but LPO in serum and liver was decreased noticeably. At level of 607.5?g/kg, all the indices were reversed; (2) A dose-dependent cancer protective effect was expressed in range of 22.5-202.5?g/kg Se of soybean selemoprotein in the diet. Feeding soybean selenoprotein can postpone the death of mice with carcinoma . Total tumour yield was consistently reduced by 78% with 202.5?g/kg supplementation; (3) Any abnormal response was never noticed during all trial by supplementing 202.5?g/kg selenoprotein. Conclusion: The increased effect of anti-oxidation and immunological modulation might be the mechanism of tumour suppression by soybean selenoprotein, and Kunming mice tolerated the soybean selenoprotein very well without any side effects.

Objective To investigate the effect of Ginsensode Rgl on the expression of Neurogranin (Ng) and behavioral alteration in cortex and hippocampus of rats with chronic stress model.Methods A total of 36 adult male SD rats were randomly divided into control group (CON),model group (CUS) and treatment group (CUS-G).The chronic stress model was established by chronic unpredictable stress.The Morris water maze was used to study the learning and memory ability.The content of Ng in cortex,hippocampus was detected by RT-PCR and Western blot.Results The water maze test showed that after chronic stress,animal learning and memory ability decreased significantly,while the treatment group rats escape latency was significantly reduced (P＜0.05);after 6 weeks of stress,the cortex and hippocampus Ng mRNA levelschronic stress rats were markedly lower than that of model rats respectively (P＜0.05,P＜0.01,P＜0.05).The cerebral cortex and hippocampus Ng mRNA levels in treatment group were significantly increased compared with that of model group respectively (P＜0.01,P＜ 0.05,P＜0.05);The cerebral cortex and hippocampus Ng levels of chronic stress rat were significantly decreased when compared with that of the model rats respectively (P＜0.05,P＜0.01,P＜0.05),The cerebral cortex and hippocampus Ng content were significantly increased in treatment group compared with the model group respectively (P＜0.01,P＜0.05).Conclusions Chronic stress can change the behaviors of nice in recognization and memory The contents of Ng and the supplement of Ginsensode Rg1 have positive adjustment.

Objective To investigate the effect of chronic stress on rat behavior changes,and specific protein neurogranin (Ng) level changes to explore the control efficiency ofginsenoside Rgl in cognitive impairment.Methods Thirty-six adult male SD rats were randomly divided into control group,model group (chronic unpredictable stress animal models,CUS),and CUS-G treatment group.The chronic stress models in the later two groups were established by CUS;rats in the CUS-G treatment group were given 11 00 mg/kg ginsenoside Rgl;Behavior changes of rats were detected by sugar consumption test and body weight measuring.Morris water maze test was used to study the learning and memory abilities.The Ng content in the cortex and hippocampus was detected by Western blotting.The Ng expression in the cortex and hippocampus was measured by immumohistochemical staining.Morphological changes in the target areas of animal models were detected by HE staining.Results As compared with the control group and CUS-G treatment group,the CUS model group had significantly decreased sugar consumption and weight (P＜0.05).The water maze test showed that learning and memory abilities in rats decreased significantly after chronic stress,and the escape latency in the CUS-G treatment group was reduced,which showed significant difference as compared with that in the control group and CUS model group (P＜0.05).As compared with the control group,the CUS model group had significantly decreased Ng content in the cerebral cortex and hippocampus and average absorbance values of Ng (P＜0.05),while the CUS-G treatment group had significantly increased Ng content in the cerebral cortex and hippocampus and average absorbance values of Ng as compared with the CUS model group (P＜0.05).Conclusions Ginsensode Rg1 (100 mg/kg) can increase the level of Ng in the cerebral cortex and hippocampus to restore the damage of cognitive ability.