Objective To investigate the relationship among the expressions of CD44v6,nm23 and MMP-9 and lymph node metastasis,differentiated degree as well as invasion depth in primary gastric carcinoma.Methods Seventy cases of gastric carcinoma,20 cases of chronic superficial gastritis,20 cases of large intesinal metaplasia and 20 cases of atipical hyperplasia were investigated using antibodies to CD44v6,MMP-9 and nm23 by S-P immunohistochemical technique.Results The positivity rates of CD44v6,MMP-9 and nm23 in gastric cancer were 64.3%,60.0%,57.1%,respectively.The positivity rates of CD44v6 and MMP-9 in gastric carcinoma were higher than that in the control groups.The positivity rates of nm23 in gastric carcinoma were lower than that in the control groups.The difference between the expression of CD44v6,MMP-9 and nm23 and lymph node metastasis was statistically significant.There was statistically significant difference between the expression of MMP-9,nm23 and the depth of invasion.CD44v6 expression in gastric cancer was positively correlated with MMP-9 expression(P

In terms of students,faculty and teaching methods,this article is involved in the problem of postgraduates'innovative ability and put up some solutions to that.The aim of the article is to help postgraduates to improve their innovative ability.

The big-data era has arrived, which involves all professions and trades. Its impact on the medical field has gradually revealed. With the characteristics of big data "4V" (volume, velocity, variety, value) and its theory generality with TCM (holistic thinking, correlativity) as well as regularity of disease development, combined with present status of acupuncture clinical research, the influences of big-data era on trial design, data collection, analysis and sharing of acupuncture clinical research are discussed in this paper, aiming to provide references for solving the difficulties of study design and determining the future research direction of acupuncture clinical research.
Acupuncture Therapy ; Biomedical Research ; Clinical Trials as Topic ; Database Management Systems ; Humans ; Medicine, Chinese Traditional

The monosaccharide composition and the structure of polysaccharide from Pichia fermentans were analyzed. The yeast cells were broken with ultrasonic disruption and the crude polysaccharide was precipitated by addition of ethanol. Then, the crude polysaccharide was separated by diethylaminoethyl(DEAE) cellulose column separation and an acquisition of uniform water-soluble polysaccharide yeast polysaccharides 5(YP5) was obtained. The results of gas chromatographic analysis indicated that the glycosyl was made of rhamnose, arabinose, xylose, mannose, glucose and galactose, whose molar ratio was 9.51∶ 1.33∶ 2.31∶ 0.94∶ 84.93∶0.96, respectively. The molecular weight of polysaccharides YP5 was 8.3×10~4 measured by Ubbelohde viscosity method. Combination of IR and ~1H NMR analysis showed that the YP5 owned characteristic infrared absorption peaks of polysaccharide and contained amino groups. The β-D-glucan was main glucosidic linkage of polysaccharides PY5.

This paper mainly studies the driving system of centrifugal blood pump for extracorporeal circulation, with the core being disc magnetic coupling. Structure parameters of disc magnetic coupling are related to the ability of transferring magnetic torque. Therefore, it is necessary to carry out disc magnetic coupling permanent magnet pole number (n), air gap length (L(g)), permanent magnet thickness (L(m)), permanent magnet body inside diameter (R(i)) and outside diameter (R(o)), etc. thoroughly. This paper adopts the three-dimensional static magnetic field edge element method of Ansys for numerical calculation, and analyses the relations of magnetic coupling each parameter to transmission magnetic torque. It provides a good theory basis and calculation method for further optimization of the disc magnetic coupling.
Extracorporeal Circulation ; Magnetic Fields ; Magnetics ; Torque

Objective To investigate the enhanced effect of polysaccharide from B.jiangsiensis(PBJ) on the immune hypofunction mouse models and its dose-effect relationship.Methods The immune hypofunction mouse models were made by hypodermic injection with cyclophosphamide(Cy) in mice.PBJ's effect on hemolysin content in mouse body induced by chicken erythrocytes and the delayed-type hypersensitivity(DTH) induced by dinitrochlorobenzene in mice were observed and contrasted in the different mouse groups treated with Cy in vivo by hypodermic injection together with PBJ at doses of 400mg/kg,200mg/kg and 50mg/kg,respectively.Results The test showed that the index of hemolysin,hemolytic plaque formation and delayed hypersensitivity reaction in model group markedly decreased while the indexes of hemolysin test in the control group,high and middle dose groups increased(P

Objective To study the therapeutic effect of tianluo decoction against hepatitis B virus(HBV) in vivo and vitro.Methods The inhibitive effect were observed in the duplication process of hepatitis B virus by fluorescence quantitative PCR(FQ-PCR) in vitro test and duck's hepatitis B animal model in vivo test.Results FQ-PCR in vitro test demonstrated that tianluo decoction had biological function to inhibit from duplication of hepatitis B virus,and there were significant difference in inhibit hepatitis B virus from duplicating in distilled water and tianluo decoction group(median:6.9?108/ml vs 2.6?104/ml,u=2.76,P

OBJECTIVE:To establish a method for simultaneous determination of 4 psoralen compounds in Buwu tincture. METHODS:HPLC was performed on the column of Dikma Diamonsil C18 with mobile phase of acetonitrile-0.2% Acetic acid by gradient elution at flow rate of 1 ml/min,detection wavelength was 246 nm,column temperature was 30 ℃,and the injection vol-ume was 15 μl. RESULTS:The linear range was 13.00-208 μg/ml for angelicin,26.00-416 μg/ml for bavachin,24.50-392 μg/ml for psoralidin and 37.88-606 μg/ml for isobavachalcone,respectively(r≥0.999 6);RSDs of precision,stability and reproducibility tests were less than 2.00%;recoveries were 95.22%-97.23%(RSD=0.87%,n=6),100.24%-104.64%(RSD=1.62%,n=6), 102.28%-104.39%(RSD=1.47%,n=6)and 97.68%-100.17%(RSD=0.97%,n=6),respectively. CONCLUSIONS:The method is simple and reproducible,and can be used for the quality control of Buwu tincture.

From August 2007 to January 2009 a total of 95 patients with complex renal calculi were treated with CT ( n = 32 ), X-ray ( n = 33 ) or ultrasound ( n = 30 ) guided percutaneous nephrostomy and ureteroscopic holmium laser lithotripsy respectively. The CT guided procedure had lower positioning time,positioning failure rate, complication rate and residual stone rate than those of X-ray and ultrasound localization, particularly had advantages in the stone clearance rate and complications rate ( all P ＜ 0. 05 ).CT guided localization can be chosen in the following conditions: higher kidney position, large staghorn calculi or renal pelvis stones without hydronephrosis, with congenital renal malformations or failure of ultrasound or X-ray localization.

There has always been no lack of discussion on Biaoben and Genjie theory in traditional Chinese medical acupuncture circles, but many questions still exist. This article expounds the original meaning of Biaoben and Genjie, traces the origin of the theory and sorts out the questions in Biaoben and Genjie theory, e.g. the difference in the position between Biaoben and Genjie, no record of the location of Genjie of six hand meridians, regularities in meridional qi circulation, understanding and valuing, and application and development to confirm the importance of Biaoben and Genjie theory and to give reference.