Leukoregulin is an immunologic hormone whose anticancer action includethe inhibition of cellular proliferation and cytolysis of tumor cells eitherdirectly or indirectly by enhancing the tumor cell's susceptibility to dest-ruction mediated by natural killer cells.The optimal condition for the ind-uction of leukoregulin Were studied by using orthogonal experimental design.The optimal concentration is 1x10~7/ml for human spleen cells;3.1?g/mlfor phytohemagglutinin(PHA);12.5ng/ml for 12-0-tetradecanoyl-phorbol-13-acetate;15% for boyin serum.Leukoregulin activity prepared by 70 hincubation is maximum,the experiment also confirmed that the inducingleukoregulin effect of PHA-PⅢis significantly high.

Objective The Da Vinci single－site surgical platform (DVSSP) is an intelligent operation platform widely used worldwide. It possesses 3D vision ,flexible operation and other advantages, so in the field of gastrointestinal surgery, it has been gradually applied to radical gastrectomy, radical gastrectomy, radical resection of colorectal cancer, gastric fundus folding, Heller myotomy, weight loss surgery and small bowel surgery, and the satisfactory clinical effect has been achieved. For gastric cancer surgery, compared with traditional laparoscopy and laparotomy, the robot operation is more accurate, flexible, and has obvious minimally invasive advantages. The intraoperative treatment and postoperative curative effect are better than the traditional laparoscopy. With the support of a large number of clinical cases, DVSSP has been proven to be a new platform for minimally invasive surgery and has considerable value in the field of gastric cancer surgery. However, there is still a long operation time and a high cost of operation. The long－term effect of gastric cancer surgery needs further observation.