1.Study on chiral recognition for the glimepiride and glimepiride-cis-isomer by applying three amino acid (D-Lysine, L-Glutamine and L-Tyrosine) as chiral probes based on electrospray ionization mass spectrometry
Ying GENG ; Caiyu ZHANG ; Tianxing DAI ; Lan HE
Drug Evaluation Research 2017;40(6):792-796
Objective Chiral recognition for the glimepiride and glimepiride-cis-isomer by applying three amino acid (D-Lysine,L-Glutamine and L-Tyrosine) as chiral probes based on electrospray ionization mass spectrometry (ESI-MS) was achieved.Methods glimepiride/glimepiride-cis-isomer solutions were mixed with three amino acid solutions.The complex was extracted by ESI-MS and then the fragmentation abundance was investigated applying collision induced dissociation (CID) by MS/MS,which is the basis of chiral recognition for glimepiride and glimepiride-cis-isomer.Results Chiral recognition effect was achieved with the recognition rate (R) 1.61,2.92 and 2.17 for D-Lysine,L-Glutamine and L-Tyrosine respectively.Conclusion 3 kinds ofchiral amino acids were used as probes to distinguish between stereoisomers,and rapid identification of glimepiride and glimepiride cis isomer by mass spectrometry come true for the first time.
2.Establishment of a non-venous bypass orthotopic liver transplantation model in Bama miniature pigs
Qiao SU ; Zhenyu YU ; Wenwen LI ; Linsen YE ; Tianxing DAI ; Rongpu LIANG ; Rongqiang LIU ; Guozhen LIN ; Guangyin ZHAO ; Wuguo LI ; Guoying WANG ; Guihua CHEN
Organ Transplantation 2019;10(1):55-
Objective To establish a non-venous bypass orthotopic liver transplantation model in Bama miniature pigs with high repeatability and stability. Methods Twelve Bama miniature pigs were randomly divided into the donor group (
3.Effect of different liver function Child-Pugh classification on clinical prognosis of hepatocellular carcinoma recipients after liver transplantation
Guozhen LIN ; Tianxing DAI ; Rongqiang LIU ; Mingbin DENG ; Guoying WANG ; Shuhong YI ; Hua LI ; Yang YANG ; Guihua CHEN
Organ Transplantation 2019;10(3):308-
Objective To evaluate the effect of the different Child-Pugh classification on the recurrence and survival of hepatocellular carcinoma (HCC) recipients after liver transplantation. Methods Clinical data of 125 HCC recipients undergoing liver transplantation were retrospectively analyzed. The 3-year disease-free survival (DFS) and overall survival (OS) rates were calculated by Kaplan-Meier survival curve. The independent risk factors probably affecting the recurrence and survival of HCC recipients after liver transplantation were identified by using Cox's proportional hazards regression model. Results The median follow-up time was 25.6 months. The 3-year DFS and OS rates were 68.4% and 65.7% for all patients. The 3-year DFS and OS rates in 113 patients with Child-Pugh class A/B HCC were 68.6% and 66.2%, whereas 66.7% and 65.6% for 12 patients with Child-Pugh class C HCC with no statistical significance (all